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91.
Javier F Chaparro-Riggers Bernard LW Loo Karen M Polizzi Phillip R Gibbs Xiao-Song Tang Mark J Nelson Andreas S Bommarius 《BMC biotechnology》2007,7(1):77
Background
The recombination of homologous genes is an effective protein engineering tool to evolve proteins. DNA shuffling by gene fragmentation and reassembly has dominated the literature since its first publication, but this fragmentation-based method is labor intensive. Recently, a fragmentation-free PCR based protocol has been published, termed recombination-dependent PCR, which is easy to perform. However, a detailed comparison of both methods is still missing. 相似文献92.