全文获取类型
收费全文 | 374篇 |
免费 | 24篇 |
出版年
2023年 | 2篇 |
2022年 | 4篇 |
2020年 | 2篇 |
2018年 | 2篇 |
2017年 | 4篇 |
2016年 | 12篇 |
2015年 | 14篇 |
2014年 | 18篇 |
2013年 | 17篇 |
2012年 | 23篇 |
2011年 | 25篇 |
2010年 | 15篇 |
2009年 | 9篇 |
2008年 | 17篇 |
2007年 | 15篇 |
2006年 | 9篇 |
2005年 | 17篇 |
2004年 | 12篇 |
2003年 | 20篇 |
2002年 | 8篇 |
2001年 | 10篇 |
2000年 | 6篇 |
1999年 | 8篇 |
1998年 | 6篇 |
1997年 | 9篇 |
1996年 | 7篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 5篇 |
1992年 | 4篇 |
1991年 | 6篇 |
1990年 | 7篇 |
1987年 | 7篇 |
1986年 | 7篇 |
1985年 | 4篇 |
1984年 | 7篇 |
1983年 | 6篇 |
1982年 | 4篇 |
1981年 | 5篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1977年 | 4篇 |
1975年 | 6篇 |
1974年 | 1篇 |
1973年 | 3篇 |
1972年 | 4篇 |
1971年 | 6篇 |
1970年 | 2篇 |
1968年 | 4篇 |
1965年 | 1篇 |
排序方式: 共有398条查询结果,搜索用时 15 毫秒
21.
Pritchard KA Ou J Ou Z Shi Y Franciosi JP Signorino P Kaul S Ackland-Berglund C Witte K Holzhauer S Mohandas N Guice KS Oldham KT Hillery CA 《American journal of physiology. Lung cellular and molecular physiology》2004,286(4):L705-L714
Vaso-occlusive events are the major source of morbidity and mortality in sickle cell disease (SCD); however, the pathogenic mechanisms driving these events remain unclear. Using hypoxia to induce pulmonary injury, we investigated mechanisms by which sickle hemoglobin increases susceptibility to lung injury in a murine model of SCD, where mice either exclusively express the human alpha/sickle beta-globin (halphabetaS) transgene (SCD mice) or are heterozygous for the normal murine beta-globin gene and express the halphabetaS transgene (mbeta+/-, halphabetaS+/-; heterozygote SCD mice). Under normoxia, lungs from the SCD mice contained higher levels of xanthine oxidase (XO), nitrotyrosine, and cGMP than controls (C57BL/6 mice). Hypoxia increased XO and nitrotyrosine and decreased cGMP content in the lungs of all mice. After hypoxia, vascular congestion was increased in lungs with a greater content of XO and nitrotyrosine. Under normoxia, the association of heat shock protein 90 (HSP90) with endothelial nitric oxide synthase (eNOS) in lungs of SCD and heterozygote SCD mice was decreased compared with the levels of association in lungs of controls. Hypoxia further decreased association of HSP90 with eNOS in lungs of SCD and heterozygote SCD mice, but not in the control lungs. Pretreatment of rat pulmonary microvascular endothelial cells in vitro with xanthine/XO decreased A-23187-stimulated nitrite + nitrate production and HSP90 interactions with eNOS. These data support the hypotheses that hypoxia increases XO release from ischemic tissues and that the local increase in XO-induced oxidative stress can then inhibit HSP90 interactions with eNOS, decreasing *NO generation and predisposing the lung to vaso-occlusion. 相似文献
22.
Morton DB Hawkins P Bevan R Heath K Kirkwood J Pearce P Scott L Whelan G Webb A;British Veterinary Association Animal Welfare Foundation;Fund for Replacement of Animals in Medical Experiments;Royal Society for the Prevention of Cruelty to Animals;Universities Federation for Animal Welfare 《Laboratory animals》2003,37(4):261-299
23.
24.
Kirkwood TB Boys RJ Gillespie CS Proctor CJ Shanley DP Wilkinson DJ 《Nature reviews. Molecular cell biology》2003,4(3):243-249
Ageing is a highly complex process; it involves interactions between numerous biochemical and cellular mechanisms that affect many tissues in an organism. Although work on the biology of ageing is now advancing quickly, this inherent complexity means that information remains highly fragmented. We describe how a new web-based modelling initiative is seeking to integrate data and hypotheses from diverse biological sources. 相似文献
25.
Inhibition of heat shock protein 90 (hsp90) in proliferating endothelial cells uncouples endothelial nitric oxide synthase activity 总被引:11,自引:0,他引:11
Dual increases in nitric oxide ((*)NO) and superoxide anion (O(2)(*-)) production are one of the hallmarks of endothelial cell proliferation. Increased expression of endothelial nitric oxide synthase (eNOS) has been shown to play an important role in maintaining high levels of (*)NO generation to offset the increase in O(2)(*-) that occurs during proliferation. Although recent reports indicate that heat shock protein 90 (hsp90) associates with eNOS to increase (*)NO generation, the role of hsp90 association with eNOS during endothelial cell proliferation remains unknown. In this report, we examine the effects of endothelial cell proliferation on eNOS expression, hsp90 association with eNOS, and the mechanisms governing eNOS generation of (*)NO and O(2)(*-). Western analysis revealed that endothelial cells not only increased eNOS expression during proliferation but also hsp90 interactions with the enzyme. Pretreatment of cultures with radicicol (RAD, 20 microM), a specific inhibitor that does not redox cycle, decreased A23187-stimulated (*)NO production and increased L(omega)-nitroargininemethylester (L-NAME)-inhibitable O(2)(*-) generation. In contrast, A23187 stimulation of controls in the presence of L-NAME increased O(2)(*-) generation, confirming that during proliferation eNOS generates (*)NO. Our findings demonstrate that hsp90 plays an important role in maintaining (*)NO generation during proliferation. Inhibition of hsp90 in vascular endothelium provides a convenient mechanism for uncoupling eNOS activity to inhibit (*)NO production. This study provides new understanding of the mechanisms by which ansamycin antibiotics inhibit endothelial cell proliferation. Such information may be useful in the development and design of new antineoplastic agents in the future. 相似文献
26.
Shanley DP Kirkwood TB 《BioEssays : news and reviews in molecular, cellular and developmental biology》2001,23(3):282-287
Menopause is an evolutionary puzzle since an early end to reproduction seems contrary to maximising Darwinian fitness. Several theories have been proposed to explain why menopause might have evolved, all based on unusual aspects of the human life history. One theory is that menopause follows from the extreme altriciality of human babies, coupled with the difficulty in giving birth due to the large neonatal brain size and the growing risk of child-bearing at older ages. There may be little advantage for an older mother in running the increased risk of a further pregnancy when existing offspring depend critically on her survival. An alternative theory is that within kin groups menopause enhances fitness by producing post-reproductive grandmothers who can assist their adult daughters. Such theories need careful quantitative assessment to see whether the fitness benefits are sufficient to outweigh the costs, particularly in circumstances of relatively high background mortality typical of ancestral environments. We show that individual theories fail this test, but that a combined model incorporating both hypotheses can explain why menopause may have evolved. 相似文献
27.
28.
29.
Tom Kirkwood 《Genetics》2014,198(1):423-424
30.
Marco Nooteboom Riem Johnson Robert W. Taylor Nicholas A. Wright Robert N. Lightowlers Thomas B. L. Kirkwood John C. Mathers Doug M. Turnbull Laura C. Greaves 《Aging cell》2010,9(1):96-99
Mitochondrial DNA (mtDNA) mutations are a cause of human disease and are proposed to have a role in human aging. Clonally expanded mtDNA point mutations have been detected in replicating tissues and have been shown to cause respiratory chain (RC) defects. The effect of these mutations on other cellular functions has not been established. Here, we investigate the consequences of RC deficiency on human colonic epithelial stem cells and their progeny in elderly individuals. We show for the first time in aging human tissue that RC deficiency attenuates cell proliferation and increases apoptosis in the progeny of RC deficient stem cells, leading to decreased crypt cell population. 相似文献