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11.
I. V. Gaikalov O. V. Ilyina S. V. Kirilchik L. V. Sukhanova 《Russian Journal of Genetics》2008,44(3):365-368
Three new microsatellite loci found for the Baikal omul Coregonus migratorius (Georgi) are described. 相似文献
12.
E. A. Ivankina A. L. Alekseeva V. F. Semeshin L. V. Omelyanchuk I. G. Palchikova N. G. Sheveleva S. V. Kirilchik I. F. Zhimulev 《Cell and Tissue Biology》2013,7(2):192-199
The genome size of Cyclops in cells at early stages of cleavage (up to the fifth division) and in somatic cells was estimated by static digital Feulgen cytophotometry in order to study quantitative changes in DNA content during chromatin diminution. Described here cytophotometric method was approbated on five different digital-imaging systems in blood cells of four vertebrate species. In all cases, we observed a direct correlation between the data obtained with known from the literature on genome size and high reproducibility, which will allow these systems to be used in future work. We also optimized the conditions for DNA hydrolysis of both blood smears and for two species of Cyclops from the Moscow population as 30 min in 5 N HCl at 24°C. Here, we first revealed chromatin diminution in two endemic Baikal species of Cyclopoida: Acanthocyclops incolotaenia and Diacyclops galbinus. We estimated the extent of chromatin diminution in Diacyclops galbinus as 95.5–96.2%. Cytometric analysis of the third species, Mesocyclops leuckarti, did not reveal obvious chromatin diminution. 相似文献
13.
V. I. Teterina N. V. Maximova T. Ya. Sitnikova S. V. Kirilchik 《Russian Journal of Genetics》2017,53(5):606-613
Baikal endemic Benedictia fragilis gastropods distributed in a wide range of depths (from sublittoral to abyssal) of three lake basins are studied. The analysis of the nucleotide sequence of the COI mitochondrial gene fragment and internal transcribed nuclear DNA spacer (ITS1) demonstrates that the studied gastropods are represented in Lake Baikal by three genetic groups. The results of the studies on genetic diversity, phenotypic traits, and distribution allow us to assume that the detected groups are incipient allopatric (geographical) species. On the basis of the data obtained and geological and climatic history of Baikal, possible pathways of the B. fragilis resettlement in the lake and the emergence of three genetic groups are hypothesized. 相似文献
14.
Koroleva A. G. Evtushenko E. V. Vershinin A. V. Zaytseva E. P. Timoshkin O. A. Kirilchik S. V. 《Molecular Biology》2020,54(4):553-562
Molecular Biology - Age-related changes in telomere length (TL) in somatic tissues are not limited only to shortening. It is known that many organisms show different TL dynamics. Such species... 相似文献