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221.
Activation of human progelatinase A by collagenase and matrilysin: Activation of procollagenase by matrilysin 总被引:2,自引:0,他引:2
Proteolytic and nonproteolytic methods were used to investigate the mechanism(s) by which human fibroblast progelatinase A and fibroblast-type procollagenase can be activated. Both collagenase and matrilysin were able to activate progelatinase A, resulting in an amino terminus in gelatinase A of Tyr.81 The cleavage occurred distal to Cys73 within the sequence of PRCGNPDVAN80-Y81NFFPRKP. While several nonproteolytic reagents were tested, only the heavy metal Hg() andp-chloromercuribenzoate (PCMB) were able to induce activation of progelatinase A and resulted in the conversion of the latent 72-kDa gelatinase A to an active form of about 64.5 kDa. Matrilysin was also able to activate procollagenase and resulted in an amino terminus in collagenase of Phe.81 These results suggest that fibroblast-type collagenase and matrilysin may be physiologically relevant activators of progelatinase A; the maintenance of latency and the process of activation for progelatinase A may occur through the cysteine-switch mechanism, and the proteolytic activation of procollagenase by matrilysin resulted in the same amino terminus as produced by stromelysin-1.Abbreviations APMA
p-aminophenylmercuric acetate
- Bistris
[bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane]
- CAPS
3-(cyclohexylamino)-1-propane sulfonic acid
- DTNB
5,5-dithiobis(2-nitrobenzoic acid)
- DTT
dithiothreitol
- EDTA
ethylenediaminetetraacetic acid
- GSSG
oxidized glutathione
- HFC
human fibroblast-type collagenase, MMP-1
- HFG
human fibroblast gelatinase A/72-kDa type IV collagenase, MMP-2
- HFS
human fibroblast stromelysin-1, MMP-3
- MMP
matrix metalloproteinase
- MT-MMP
membrane-type matrix metalloproteinase, MMP-14
- NEM
N-ethylmaleimide
- PCMB
p-chloromercuribenzoate
- PMA
phenylmercuric acetate
- PMC
phenylmercuric acid
- PMSF
phenylmethanesulfonyl fluoride
- PTH
phenylthiohydantoin
- RTT
rat tail tendon
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
- Tes
[tris(hydroxymethyl)-methyl-2-aminoethane sulfonic acid]
- TIMP
tissue inhibitor of metalloproteinase
- Tris
tris(hydroxymethyl)aminomethane
- Tricine
N-[tris(hydroxymethyl)methyl]glycine 相似文献
222.
Ferric-binding proteins (FbpA) have been implicated in the transferrin receptor-mediated iron acquisition pathways of Haemophilus influenzae and Neisseria spp. These proteins are believed to function by shuttling iron from outer membrane transferrin receptors to a specific inner membrane permease complex. However, the role of these proteins has not been conclusively resolved, as attempts at creating isogenic mutants in the fbpA genes of both species have been unsuccessful, prompting the hypothesis that FbpA may play a critical role in H . influenzae and Neisseria spp. This study describes the construction and characterization of an H . influenzae isogenic fbpA mutant. It is demonstrated that this mutant is deficient in its ability to use human transferrin as a sole iron source, even though the strain is still competent for binding human transferrin. It is also demonstrated that this mutant is impaired in its ability to use ferric citrate as an iron source, and grows at a reduced rate relative to wild type in broth supplemented with protoporphyrin rather than haemin. 相似文献
223.
Interchild variability in breathing patterns may contribute to variability in fine particle lung deposition and morbidity in children associated with those particles. Fractional deposition (DF) of fine particles (2-microm monodisperse, carnauba wax particles) was measured in healthy children, age 6-13 yr (n = 36), while they followed a resting breathing pattern previously determined by respiratory inductance plethysmography. Interchild variation in DF, measured by photometry at the mouth, was most strongly predicted by their tidal volume (Vt) (r =0.79, P < 0.001). Multiple regression analysis further showed that, for any given height and age, Vt increased with increasing body mass index (BMI) (P < 0.001). The overweight children (> or =95th percentile BMI) (n = 8) had twice the DF of those in the lowest BMI quartile (<25th percentile) (n = 9; 0.28 +/- 0.13 vs. 0.15 +/- 0.06, respectively; P < 0.02). In the same groups, resting minute ventilation was also significantly higher in the overweight children (8.5 +/- 2.2 vs. 5.9 +/- 1.1 l/min; P < 0.01). Consequently, the rate of deposition (i.e., particles depositing/time) in the overweight children was 2.8 times that of the leanest children (P < 0.02). Among all children, the rate of deposition was significantly correlated with BMI (r = 0.46, P = 0.004). These results suggest that increased weight in children may be associated with increased risk from inhalation of pollutant particles in ambient air. 相似文献
224.
225.
The time course of glycogen changes in soleus muscle recovering from 3 days of nonweight bearing by hindlimb suspension was investigated. Within 15 min and up to 2 h, muscle glycogen decreased. Coincidentally, muscle glucose 6-phosphate and the fractional activity of glycogen phosphorylase, measured at the fresh muscle concentrations of AMP, increased. Increased fractional activity of glycogen synthase during this time was likely the result of greater glucose 6-phosphate and decreased glycogen. From 2 to 4 h, when the synthase activity remained elevated and the phosphorylase activity declined, glycogen levels increased (glycogen supercompensation). A further increase of glycogen up to 24 h did not correlate with the enzyme activities. Between 24 and 72 h, glycogen decreased to control values, possibly initiated by high phosphorylase activity at 24 h. At 12 and 24 h, the inverse relationship between glycogen concentration and the synthase activity ratio was lost, indicating that reloading transiently uncoupled glycogen control of this enzyme. These data suggest that the activities of glycogen synthase and phosphorylase, when measured at physiological effector levels, likely provide the closest approximation to the actual enzyme activities in vivo. Measurements made in this way effectively explained the majority of the changes in the soleus glycogen content during recovery from nonweight bearing. 相似文献
226.
Coupling of cardiac and locomotor rhythms 总被引:1,自引:0,他引:1
Kirby R. L.; Nugent S. T.; Marlow R. W.; MacLeod D. A.; Marble A. E. 《Journal of applied physiology》1989,66(1):323-329
The pressure within exercising skeletal muscle rises and falls rhythmically during normal human locomotion, the peak pressure reaching levels that intermittently impede blood flow to the exercising muscle. Speculating that a reciprocal relationship between the timing of peak intramuscular and pulsatile arterial pressures should optimize blood flow through muscle and minimize cardiac load, we tested the hypothesis that heart rate becomes entrained with walking and running cadence at some locomotion speeds, by means of electrocardiography and an accelerometer to provide signals reflecting heart rate and cadence, respectively. In 18 of 25 subjects, 1:1 coupling of heart and step rates was present at one or more speeds on a motorized treadmill, generally at moderate to high exercise intensities. To determine how exercise specific this phenomenon is, and to refute the competing hypothesis that coupling is due to vertical accelerations of the heart during locomotion, we had 12 other subjects cycle on an electronically braked bicycle ergometer. Coupling was found between heart rate and pedaling frequency in 10 of them. Cardiac-locomotor coupling appears to be a normal physiological phenomenon, and its identification provides a fresh perspective from which to study endurance. 相似文献
227.
Cyanide detoxification in mammals occurs, in part, by sulfur transfer by rhodanese to form the less toxic thiocyanate. Thiosulfate and nitrite are often used in combination for the treatment of cyanide intoxication. This report shows that nitrite can inhibit the rate of sulfur transfer by rhodanese in vitro. Nitrate, chloride, sulfate, and acetate were also examined as inhibitors. Inhibition by nitrite appeared to be more complex than for the other anions tested. Closer examination showed that nitrite can inactivate the sulfur-free rhodanese. Our observation leads to the suggestion that, in vivo, either rhodanese is maintained in its more stable sulfur-substituted form or cellular compartmentalization prevents inactivation by nitrite. 相似文献
228.
S. Bradbury W. D. Billington D. R. S. Kirby E. A. Williams 《The Histochemical journal》1970,2(4):263-274
Synopsis The surface coating of normal and abnormal trophoblast has been studied histochemically. It has been found to contain a highly sulphated mucosaccharide which is probably present together with an acidic non-sulphated mucosaccharide; there are suggestions that the accompanying protein is highly basic. The only difference noted between the coat on the normal trophoblast and that of the hydatidiform mole and ectopic trophoblast was the presence ofvis-glycol groups in the normal which were not found in the abnormal. It is suggested that the presence of the sulphated mucoprotein on the surface of the trophoblast is involved in the protection of the trophoblast from immunological attack. 相似文献
229.
Summary When 3H-L-fucose is provided to corn roots, a large proportion of the radioactivity is recovered in the polysaccharides extracted from the cell wall. Hydrolysis of this material yields 3H-L-fucose as the sole radioactive product. Two metabolites, identified tentatively as L-fucose-1-phosphate and a nucleoside diphosphate derivative of L-fucose have been isolated from the ethanol soluble fractions of the roots and are possibly precursors of the polysaccharide. Autoradiographs of tissue sections indicate that the synthesis of polysaccharides containing L-fucose is confined largely to the root-cap and epidermis. The outer epidermal wall and root-cap slime are particularly radioactive and, therefore, likely to be relatively rich in fucose. By contrast the cell walls from more deeply lying tissues incorporate negligible amounts of the sugar. 相似文献
230.
Ronald J. Przybylski Vilma Szigeti Albert C. Kirby 《In vitro cellular & developmental biology. Plant》1986,22(7):402-406
Summary Lanthanum has been used effectively in studies of calcium physiology in experiments of short duration. In experiments of longer
duration, we report that solutions, such as cell culture medium, containing lanthanum (La++) undergo a decrease in pH on the time scale of hours. Presumaly, the decrease in pH is a consequece of the hydrolysis of
water by the solution-active La+++ ions. We have devised a defined culture medium without serum and chick embryo extract which is permissive for myotube formation.
This defined medium is also useful for studies of La+++ as a calcium antagonist. with Ca++ to low-Ca++ fusion-blocked cultures.
This study was supported in part by NIH grants NS 10196 and AM 25202 and The Muscular Dystrophy Association. 相似文献