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排序方式: 共有153条查询结果,搜索用时 31 毫秒
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Holger Maier Christine Schütt Ralph Steinkamp Anja Hurt Elida Schneltzer Philipp Gormanns Christoph Lengger Mark Griffiths David Melvin Neha Agrawal Rafael Alcantara Arthur Evans David Gannon Simon Holroyd Christian Kipp Navis Pretheeba Raj David Richardson Sophie LeBlanc Laurent Vasseur Hiroshi Masuya Kimio Kobayashi Tomohiro Suzuki Nobuhiko Tanaka Shigeharu Wakana Alison Walling David Clary Juan Gallegos Helmut Fuchs Martin Hrabě de Angelis Valerie Gailus-Durner 《Mammalian genome》2015,26(9-10):467-481
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Humphries LA Dangelmaier C Sommer K Kipp K Kato RM Griffith N Bakman I Turk CW Daniel JL Rawlings DJ 《The Journal of biological chemistry》2004,279(36):37651-37661
Tyrosine phosphorylation of phospholipase Cgamma2 (PLCgamma2) is a crucial activation switch that initiates and maintains intracellular calcium mobilization in response to B cell antigen receptor (BCR) engagement. Although members from three distinct families of non-receptor tyrosine kinases can phosphorylate PLCgamma in vitro, the specific kinase(s) controlling BCR-dependent PLCgamma activation in vivo remains unknown. Bruton's tyrosine kinase (Btk)-deficient human B cells exhibit diminished inositol 1,4,5-trisphosphate production and calcium signaling despite a normal inducible level of total PLCgamma2 tyrosine phosphorylation. This suggested that Btk might modify a critical subset of residues essential for PLCgamma2 activity. To evaluate this hypothesis, we generated site-specific phosphotyrosine antibodies recognizing four putative regulatory residues within PLCgamma2. Whereas all four sites were rapidly modified in response to BCR engagement in normal B cells, Btk-deficient B cells exhibited a marked reduction in phosphorylation of the Src homology 2 (SH2)-SH3 linker region sites, Tyr(753) and Tyr(759). Phosphorylation of both sites was restored by expression of Tec, but not Syk, family kinases. In contrast, phosphorylation of the PLCgamma2 carboxyl-terminal sites, Tyr(1197) and Tyr(1217), was unaffected by the absence of functional Btk. Together, these data support a model whereby Btk/Tec kinases control sustained calcium signaling via site-specific phosphorylation of key residues within the PLCgamma2 SH2-SH3 linker. 相似文献
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Developmental Expression Patterns of Arabidopsis XTH Genes Reported by Transgenes and Genevestigator 总被引:1,自引:0,他引:1
The plant cell wall is the structural basis of cellular form and thus forms a foundation on which morphogenesis builds organs
and tissues. Enzymes capable of modifying major wall components are prominent candidates for regulating wall form and function.
Xyloglucan endotransglucosylases/hydrolases (XTHs) are predicted to participate in xyloglucan integration and/or restructuring.
XTHs are encoded by large gene families in plants; the Arabidopsis genome encodes 33 XTHs. To gain insight into the potential
physiological relevance of the distinct members of this family, GUS reporter fusion genes were constructed, and plants expressing these transgenes were characterized to reveal spatial and temporal
patterns of expression. In addition, Genevestigator sources were mined for comprehensive and comparative XTH expression regulation analysis. These data reveal that the Arabidopsis XTHs are likely expressed in every developmental stage from seed germination through flowering. All organs show XTH::GUS expression and most, if not all, are found to express multiple XTH::GUS genes. These data suggest that XTHs may contribute to morphogenesis at every developmental stage and in every plant organ.
Different XTHs have remarkably diverse and distinct expression patterns indicating that paralogous genes have evolved differential expression
regulation perhaps contributing to the maintenance of the large gene family. Extensive overlap in XTH expression patterns is evident; thus, XTHs may act combinatorially in determining wall properties of specific tissues or
organs. Knowledge of gene-specific expression among family members yields evidence of where and when gene products may function
and provides insights to guide rational approaches to investigate function through reverse genetics.
Electronic supplementary material Electronic supplementary material is available for this article at
and accessible for authorised users. 相似文献
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Zi-Feng Jiang Fangfang Xia Kipp W. Johnson Elizabeth Bartom Jigyasa H. Tuteja Rick Stevens Robert L. Grossman Marina Brumin Kevin P. White Murad Ghanim 《Journal of bacteriology》2012,194(23):6678-6679
“Candidatus Portiera aleyrodidarum” is the obligate primary endosymbiotic bacterium of whiteflies, including the sweet potato whitefly Bemisia tabaci, and provides essential nutrients to its host. Here we report two complete genome sequences of this bacterium from the B and Q biotypes of B. tabaci. 相似文献
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T. WORTHINGTON P. S. KEMP P. E. OSBORNE A. DILLEN J. COECK M. BUNZEL‐DRÜKE M. NAURA J. GREGORY K. EASTON 《Freshwater Biology》2012,57(3):602-611
1. Availability of suitable habitat is a prerequisite for species reintroduction success, and to ensure population persistence, investigations of a species’ habitat utilisation throughout its life history should be conducted as part of a feasibility study. 2. Habitat utilisation models for burbot, Lota lota, developed using data from field studies conducted in France and Germany and information from the literature were used to assess the feasibility of reintroducing burbot into rivers of its former native range in eastern England. 3. Per cent tree roots, aquatic vegetation and flow types were important predictors of adult burbot abundance. Furthermore, the habitat utilisation models were supplemented with information from the literature, which suggested that off‐channel habitat such as wetlands and backwaters is important for spawning and nursery stages. 4. An assessment of the habitat availability in the rivers of the burbot’s former native range using variables related to spawning and nursery and adult life stages showed that although adult habitat was widely distributed, the availability of spawning and nursery habitat was less abundant, potentially limiting successful reestablishment. 5. Potential suitable habitat was concentrated in the central and southern areas of the species’ former English distribution. Overall, rivers of the burbot’s former range potentially afford suitable habitat to sustain a reintroduced population. However, sites should be preferentially selected on the basis of having appropriate spawning and nursery areas. 相似文献
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Phosphatidylcholine 36:1 concentration decreases along with demyelination in the cuprizone animal model and in post‐mortem multiple sclerosis brain tissue 下载免费PDF全文
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