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11.
Y Shinoda A Shouji T Yamane A Suzuki T Ashida H Kihara M Ohno 《Journal of biochemistry》1990,107(1):84-86
Crystallization and a preliminary crystallographic study of Trimeresurus flavoviridis (habu snake) phospholipase A2 (PLA2) were carried out. Although crystals were obtained from various solutions, crystals suitable for X-ray analysis could be obtained from polyethylene glycol solutions only when a repeated seeding technique was applied starting from twinned crystals. The crystal is monoclinic with space group P21, with a = 44.1, b = 55.7, c = 48.8 A, and beta = 92.4 degrees. An asymmetric unit contains a dimer consisting of two identical subunits made of 122 amino acids. The crystal reflects X-rays beyond 2.5 A. A Pt derivative gave a good isomorphous crystal. 相似文献
12.
T. Seimiya M. Ashida T. Muramatsu I. Hara M. Hayashi 《Chemistry and physics of lipids》1978,22(3):221-226
Radiotracer studies of calcium adsorption to dipalmitoylphosphatidyl-alkanolamine monolayers measured at various temperatures showed that the binding constant of calcium increased with temperature up to around 30°C but then decreased on exceeding this critical temperature.The temperature dependent ionic structure of ampholytic phospholipid monolayers are discussed. 相似文献
13.
Localization of anchor loci representing five hundred annotated rice genes to wheat chromosomes using PLUG markers 总被引:1,自引:0,他引:1
Goro Ishikawa Toshiki Nakamura Taizo Ashida Mika Saito Shuhei Nasuda Takashi R. Endo Jianzhong Wu Takashi Matsumoto 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(3):499-514
PCR-based Landmark Unique Gene (PLUG) markers are EST-PCR markers developed based on the orthologous gene conservation between
rice and wheat, and on the intron polymorphisms among the three orthologous genes derived from the A, B and D genomes of wheat.
We designed a total of 960 primer sets from wheat ESTs that showed high similarity with 951 single-copy rice genes. When genomic
DNA of Chinese Spring wheat was used as a template, 872 primer sets amplified one to five distinct products. Out of these
872 PLUG markers, 531 were assigned to one or more chromosomes by nullisomic-tetrasomic analysis. For each wheat chromosome,
the number of loci detected ranged from 32 for chromosome 6A to 73 for chromosome 7D, with an average of 48 loci per chromosome.
Several novel synteny perturbations were identified using deletion bin-mapping of markers. Furthermore, we demonstrated that
PLUG markers can be used as probes to simultaneously identify BAC clones that contain homoeologous regions from all three
genomes.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
14.
Target enzymes on hepatic dysfunction caused by dietary products of lipid peroxidation 总被引:3,自引:0,他引:3
Dietary products of lipid peroxidation cause hepatic dysfunction due to decreases in the activities of some hepatic enzymes and to depletion of CoA. An idea about the decreases and depletion is that the enzymes and CoA could be injured directly by the incorporated products in the liver. Their inactivations in vitro were then examined using a reasonable amount of peroxidation products. The hepatic cytosol, microsomes, and mitochondria were incubated with 10, 15, and 20 micrograms/mg protein of peroxidation products, respectively, and changes in the enzymatic activities were monitored. Glucose-6-phosphate dehydrogenase, mitochondrial NAD-dependent aldehyde dehydrogenase, glucokinase, and glyceradehyde phosphate dehydrogenase were inactivated, and the CoA level was decreased, but the other hepatic enzymes were not. Although glyceraldehyde phosphate dehydrogenase was most sensitive to peroxidation products in vitro, the decrease in activity was not detected by the oral dose of secondary products. On the other hand, among the components of peroxidation products, hydroperoxides and polymers are not incorporated in the liver, but decomposed products of low molecular weight are incorporated. Glucokinase among the above enzymes was not inactivated by the low-molecular-weight products. It was therefore concluded that glucose-6-phosphate dehydrogenase, mitochondrial NAD-dependent aldehyde dehydrogenase, and CoA were targets of the direct attack by incorporated components of peroxidation products in the liver. 相似文献
15.
Satoshi Takasugi Kinya Ashida Suyaka Maruyama Yukari Matsukiyo Tetsuo Kaneko Taketo Yamaji 《Biological trace element research》2013,153(1-3):309-318
This study aimed to investigate the effects of a combination of a dairy product fermented by lactobacilli (DFL) and galactooligosaccharides (GOS) on mineral balances in growing rats with hypochlorhydria induced by a proton pump inhibitor (PPI). Three-week-old male rats were assigned to receive one of six diets: a control diet, control diets containing 1.6 or 5.0 % GOS, a DFL diet and DFL diets containing 1.6 or 5.0 % GOS for 9 days. From day 5 of the feeding period, half of the rats fed with control diets were subcutaneously administered with saline, whereas the remaining rats were administered with PPI for 5 days. Calcium (Ca), phosphorus (P), magnesium (Mg), iron (Fe) and zinc (Zn) balances were determined from days 6 to 9. PPI administration significantly decreased the apparent absorption of Ca and Fe and increased urinary P excretion, resulting in decreased Ca, Fe and P retention. GOS dose-dependently increased the apparent absorption of Ca, Mg and Fe and urinary Mg excretion and decreased urinary P excretion. DFL significantly increased the apparent absorption of Ca and Mg and urinary Mg excretion. The combination of DFL and GOS additively affected these parameters, resulting in increased Ca, P and Fe retention, and it further increased the apparent absorption and retention of Zn at 5.0 % GOS. In conclusion, the combination of DFL and GOS improves Ca, P and Fe retention in an additive manner and increases the Zn retention in growing rats with hypochlorhydria induced by PPI. 相似文献
16.
Fatty livers were produced by feeding rats an amino acid imbalanced diet containing 8% of casein supplemented with 0.3% of DL-methionine, and the oxidative phosphorylation of liver mitochondria and homogenates was examined. In contrast to choline deficiency, the mitochondria and the homogenates of fatty liver resulting from such an amino acid imbalance showed almost normal P:O ratio. However, the response to an osmotic treatment of liver mitochondria of rats fed the amino acid imbalanced diet was different from that of normal rats. 相似文献
17.
18.
Studies were made on the incorporation of 14C of acetate-1-14C into the lipids of the liver and carcass, and changes in the concentrations of nucleotides, citric acid, and free fatty acids in the liver, using rats fed diets consisting of starch and casein, or starch and corn oil, at different ratios. Lipogenesis was stimulated with an increase in the content of starch both in the starch-casein and starch-corn oil diets. There was a rapid drop in lipogenesis in rats fed the diet with a little increment in the content of corn oil. Lipogenesis decreased gradually with an increment in the content of casein in the starch-casein diet. A positive correlation was found between lipogenesis and the level of ATP in the liver. The concentration of citric acid decreased with an increase in lipogenesis in the liver. Changes in dietary composition did not produce any significant alteration in the concentration of free fatty acids in the liver. 相似文献
19.
Hisashi Ashida Kana Tanigawa Masashi Kiyohara Toshihiko Katoh Takane Katayama 《Bioscience, biotechnology, and biochemistry》2013,77(11):2030-2039
ABSTRACTSialidases catalyze the removal of terminal sialic acid from various complex carbohydrates. In the gastrointestinal tract, sialic acid is commonly found in the sugar chain of mucin, and many enteric commensals use mucin as a nutrient source. We previously identified two different sialidase genes in Bifidobacterium bifidum, and one was cloned and expressed as an extracellular protein designated as exo-α-sialidase SiaBb2. The other exo-α-sialidase gene (siabb1) from the same bifidobacterium encodes an extracellular protein (SiaBb1) consisting of 1795 amino acids with a molecular mass of 189 kDa. SiaBb1 possesses a catalytic domain that classifies this enzyme as a glycoside hydrolase family 33 member. SiaBb1 preferentially hydrolyzes α2,3-linked sialic acid over α2,6-linked sialic acid from sialoglycan, which is the same as SiaBb2. However, SiaBb1 has an SGNH hydrolase domain with sialate-O-acetylesterase activity and an N-terminal signal sequence and C-terminal transmembrane region. SiaBb1 is the first bifunctional sialidase identified with esterase activity.Abbreviations: GalNAc: N-acetyl-D-galactosamine; Fuc: L-fucose; Gal: D-galactose 相似文献
20.
Dinh T. Lam Bui C. Buu Nguyen T. Lang Kinya Toriyama Ikuo Nakamura Ryuji Ishikawa 《Ecology and evolution》2019,9(5):2964-2977
Oryza rufipogon Griff. is a perennial species of wild rice widely distributed along the channels and rivers of the Mekong Delta, Vietnam. This study attempted to find centers of diversity among wild rice populations in this area and their inter‐relationships. The highest genetic diversity was found in the Dong Thap population and the lowest in the Can Tho population. Maternal diversity evaluated using chloroplast INDELs detected ten plastid types, five of which were novel relative to other Asian countries. The mitochondrial genome suggested two unique deletions. One 699‐bp deletion via short tandem repeats was accompanied by another deletion including orf153. All accessions carrying the mitochondrial type were found in a particular plastid type. This unique maternal lineage was confined to specific channels where it showed vigorous vegetative growth in comparison to upstream areas where various maternal lineages and maximum genetic diversity occurred. This area along the Mekong Delta is a center of not only nuclear but also maternal diversity. 相似文献