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61.
62.
NMR imaging of heavy metal absorption in alginate, immobilized cells, and kombu algal biosorbents 总被引:1,自引:0,他引:1
In this contribution, an NMR imaging study of heavy metal absorption in alginate, immobilized-cell biosorbents, and kombu (Laminaria japonica) algal biomass is presented. This method provides the good possibility of directly monitoring the time evolution of the spatial distribution of the ions in the materials. From these results, we demonstrate that rare earth ions are absorbed with a steep reaction front that can be described very well with a modified shrinking core model, while copper ions are absorbed with a more diffuse front. 相似文献
63.
Kappa-chain constant-region gene sequences in genus Rattus: coding regions are diverging more rapidly than noncoding regions 总被引:2,自引:0,他引:2
We have determined the nucleotide sequence of a 1,200-base pair (bp)
genomic fragment that includes the kappa-chain constant-region gene (C
kappa) from two species of native Australian rodents, Rattus leucopus
cooktownensis and Rattus colletti. Comparison of these sequences with each
other and with other rodent C kappa genes shows three surprising features.
First, the coding regions are diverging at a rate severalfold higher than
that of the nearby noncoding regions. Second, replacement changes within
the coding region are accumulating at a rate at least as great as that of
silent changes. Third, most of the amino acid replacements are localized in
one region of the C kappa domain--namely, the carboxy-terminal "bends" in
the alpha-carbon backbone. These three features have previously been
described from comparisons of the two allelic forms of C kappa genes in R.
norvegicus. These data imply the existence of considerable evolutionary
constraints on the noncoding regions (based on as yet undetermined
functions) or powerful positive selection to diversify a portion of the
constant-region domain (whose physiological significance is not known).
These surprising features of C kappa evolution appear to be characteristic
only of closely related C kappa genes, since comparison of rodent with
human sequences shows the expected greater conservation of coding regions,
as well as a predominance of silent nucleotide substitutions within the
coding regions.
相似文献
64.
Summary The role played by transmembrane K+ gradients in providing an energy input for Na+-dependent monosaccharide transport systems was evaluated with the use of isolated intestinal epithelial cells. Experimentally imposing a K+ gradient in a sense reversed from normal did not lead to extrusion of sugar from cells which had been pre-equilibrated with14C-3-OMG, even in situations where a reversed Na+ gradient was also imposed. Furthermore, cells preloaded with K+ have no better ability to accumulate 3-OMG than do cells depleted of K+, when the two populations are compared under identical incubation conditions. Fluxes of K+ associated with the sugar carrier could not be detected in terms of suspected sensitivity to agents which immobilize the sugar carrier. In addition, fluxes of sugar in response to imposed K+ gradients were not demonstrable in cells de-energized by preincubation with DNP, no matter in which direction the K+ gradient was imposed. Finally, the severe inhibitory effects of K+ on Na+-dependent sugar transport by the cells disappears in de-energized cells, despite the fact that Na+-dependent carrier-mediated sugar entry still occurs. All of these facts are difficult to reconcile with a significant role for cellular K+ gradients in supporting active sugar transport as envisioned by the ion gradient hypothesis. We have suggested instead a fundamental Na+-dependent energy transductive event which depends on ATP, and which can generate a membrane-bound energized intermediate which serves to support a variety of active transport events. An analogy is drawn between this concept for animal cell plasma membranes and the better documented phosphotransferase system for sugar transport described for certain microorganisms. 相似文献
65.
Interaction between Na+-dependent transport systems for sugars and amino acids. Evidence against a role for the sodium gradient 总被引:1,自引:0,他引:1
Summary The concept that interaction between sodium-dependent transport systems represents competition for energy inherent in the transmembrane sodium gradient was examined with the use of isolated intestinal epithelial cells. The isolated cells exhibit transport interactions which are more significant in magnitude than those which have been described for intact tissue preparations. Accumulation of 1mm valine is inhibited 60% by 10mm 3-OMG. Conversely, uptake of 1mm 3-OMG is inhibited only 20% by 10mm valine. These data suggest that 3-OMG must discharge the cellular Na+ gradient more effectively than valine, if Na+ gradient dissipation can be taken as a basis for the inhibitory interaction. However, entry of 10mm 3-OMG is significantly slower than the entry of 10mm valine. Even if appropriate corrections are made for passive substrate entry and for differences in Na/substrate entry stoichiometry, it appears that valine should be somewhat more effective than 3-OMG in discharging the Na+ gradient. In light of these facts, it seems unlikely that the mechanistic basis for interaction between sugar and amino acid transport systems can be related to concomitant co-entry of Na+. It is suggested that the interaction results instead from competition for energized intermediates generated at limited rates by basic energy transduction events associated with the cell membrane which serve in support of a variety of active transport systems. 相似文献
66.
GA Bray 《Obesity (Silver Spring, Md.)》1997,5(3):271-274
It is often difficult to identify the ‘who, when, and where’ of advances in medicine and surgery because it's a rare advance indeed (such as the use of digitalis by William Withering) that can be clearly related to the astuteness of one person at one time and place. 相似文献
67.
A Hidden Markov Model approach to variation among sites in rate of evolution 总被引:40,自引:20,他引:20
The method of Hidden Markov Models is used to allow for unequal and unknown
evolutionary rates at different sites in molecular sequences. Rates of
evolution at different sites are assumed to be drawn from a set of possible
rates, with a finite number of possibilities. The overall likelihood of
phylogeny is calculated as a sum of terms, each term being the probability
of the data given a particular assignment of rates to sites, times the
prior probability of that particular combination of rates. The
probabilities of different rate combinations are specified by a stationary
Markov chain that assigns rate categories to sites. While there will be a
very large number of possible ways of assigning rates to sites, a simple
recursive algorithm allows the contributions to the likelihood from all
possible combinations of rates to be summed, in a time proportional to the
number of different rates at a single site. Thus with three rates, the
effort involved is no greater than three times that for a single rate. This
"Hidden Markov Model" method allows for rates to differ between sites and
for correlations between the rates of neighboring sites. By summing over
all possibilities it does not require us to know the rates at individual
sites. However, it does not allow for correlation of rates at nonadjacent
sites, nor does it allow for a continuous distribution of rates over sites.
It is shown how to use the Newton-Raphson method to estimate branch lengths
of a phylogeny and to infer from a phylogeny what assignment of rates to
sites has the largest posterior probability. An example is given using
beta-hemoglobin DNA sequences in eight mammal species; the regions of high
and low evolutionary rates are inferred and also the average length of
patches of similar rates.
相似文献
68.
Summary Phlorizin binding is studied in isolated intestinal epithelial cells of the chick. Cells are ATP depleted to allow extensive manipulation of ionic gradients and membrane potential (). Phlorizin binding is assayed at steady state. Carrier specific phlorizin binding is defined asd-glucose (90 mM) inhibitable binding. Specific binding displays simple Michaelian kinetics as a function of phlorizin. indicating the presence of a single homogeneous binding site. Sodium concentrations and modify the apparent binding affinity but not the maximum number of binding sites. In contrast, the activation curve as a function of sodium concentrations is sigmoid and the apparent maximum number of binding sites at saturating sodium is phlorizin dependent. The rate of phlorizin association is both and sodium-concentration dependent. Dissociation is sodium-concentration dependent but not dependent. Theoretical analysis indicates binding order of substrates is random. In addition, data suggests that the phlorizin/sodium stoichiometry is 2:1. The dependence can be explained by two models: either translocation is the -dependent step and the free carrier is anionic, or sodium binding is the -dependent step. 相似文献
69.
The molecular mechanism and potential dependence of the Na+/glucose cotransporter. 总被引:1,自引:1,他引:0 下载免费PDF全文
Activity of the Na+/glucose cotransporter endogenously expressed in LLC-PK1 cells was measured using whole cell recording techniques under three different sodium concentration conditions: 1) externally saturating, zero trans; 2) 40 mM external, zero trans; and 3) externally saturating, 50 mM trans. Activity of the transporter with increasing concentrations of sugar was measured for each set of conditions, from which the maximal current for saturating sugar, Im, was determined. The Im measured shows substantial potential dependence for each set of conditions. The absolute Im and the relative potential dependence of Im compared among the various solute conditions were used to identify which loci in the transport cycle are responsible for potential-dependent changes in function. The experimental data were compared with the predicted Im values calculated from an eight-state, sequential, reversible model of a transport reaction kinetic scheme. Predictions derived from assignment of rate limitation and/or potential dependence to each of the 16 transitions in the transport pathway were derived and compared with the measured data. Most putative models were dismissed because of lack of agreement with the measured data, indicating that several steps along the transport pathway are not rate limiting and/or not potential dependent. Only two models were found that can completely account for the measured data. In one case, translocation of the free carrier must be rate limiting, and both extracellular sodium-binding events as well as translocation of both free and fully loaded carrier forms must be potential-dependent transitions. In the second case, translocation of the free carrier and dissociation of the first sodium to be released intracellularly must be equivalently rate limiting. In this case only the two translocation events are required to be potential dependent. The two external sodium-binding events might still be potential dependent, but this is not required to fit the data. Previous reports suggest that the first model is correct; however, no direct experimental data compel us to dismiss the second option as a feasible model. 相似文献
70.