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981.
Ibrahimova Ulkar Zivcak Marek Gasparovic Kristina Rastogi Anshu Allakhverdiev Suleyman I. Yang Xinghong Brestic Marian 《Photosynthesis research》2021,150(1-3):195-211
Photosynthesis Research - Effects of salinity caused by 150 mM NaCl on primary photochemical reactions and some physiological and biochemical parameters (K+/Na+ ratio, soluble sugars,... 相似文献
982.
983.
Drabik Magdalena Głuszak Mariusz Wróblewska-Łuczka Paula Plewa Zbigniew Jankiewicz Marek Kozińska Justyna Florek-Łuszczki Magdalena Plech Tomasz Łuszczki Jarogniew J. 《Neurochemical research》2021,46(2):396-410
Neurochemical Research - Protective (antiseizure) effects of 4-butyl-5-[(4-chloro-2-methylphenoxy)-methyl]-2,4-dihydro-3H-1,2,4-triazole-3-thione (TPL-16) and acute neurotoxic effects were... 相似文献
984.
Muhammad Shahid Nisar Muhammad Awais Ismail Hassan Ramzan Muhammad Mudassar Maqbool Tasneem AHMAD Hamed A. Ghramh Ahlam Khalofah Jaroslav Kmet Marek Horvát Shahid Farooq 《Saudi Journal of Biological Sciences》2021,28(7):3880-3885
Housefly is a significant domestic pest, which causes nuisance. The use of insecticides is discouraged to manage housefly; therefore, alternative management strategies are inevitable. The current study investigated the impact of different plant extracts, i.e., Moringa oleifera (moringa), Allium sativum (garlic) and Piper nigrum (black pepper) on biological parameters of house fly. Two different concentrations (i.e., 25 and 50%) of the extracts were blended in larval diet made through mixing of wheat bran, yeast and dried milk powder. The results indicated significant differences for larval duration. Maximum larval duration was recorded for garlic followed by black pepper and moringa, respectively. In case of pupal duration, non-significant differences were observed among plant extracts. Increase rate of oviposition was noticed with moringa at 25% concentration, while decreased oviposition rate was noted for garlic with 50% concentration. Egg hatching percentage remained non-significant for the botanical extracts. The highest survival was observed with moringa, while garlic resulted in the lowest survival. The highest repellency was noticed for garlic followed by black pepper, whereas moringa resulted in the lowest repellency after 30, 60 and 90 min. Prolonged developmental time was observed for bot concentrations of garlic, whereas moringa noted the shortest developmental time. Thus moringa was found to be a promoter of housefly development. Minimum adult emergence was found with both concentrations of garlic followed by 50% concentration of black pepper. The 50% concentration of black pepper promoted the population of adult males, while both concentrations of moringa and 25% concentration of black pepper encouraged the population of female adults. Study outcomes depicted that tested botanical extracts had significant potential for disturbing biological parameters of housefly. The garlic extracts can potentially be used to manage housefly. However, further investigations on the larval and adult mortality are needed. 相似文献
985.
Daniela Brünnert Vijay Kumar Vibha Kaushik Jens Ehrhardt Kirti Raj Chahar Phulwanti Kumari Sharma Marek Zygmunt Pankaj Goyal 《Reproductive biology》2021,21(3):100516
Cytokines’ secretion from the decidua and trophoblast cells has been known to regulate trophoblast cell functions, such as Extravillous trophoblasts (EVTs) cell migration and invasion and remodeling of spiral arteries. Defective angiogenesis and spiral arteries transformation are mainly caused by proinflammatory cytokines and excessive thrombin generation during preeclampsia. Monocyte chemotactic protein-1 (MCP-1), a crucial cytokine, has a role in maintaining normal pregnancy. In this study, we explored whether thrombin regulates the secretion of MCP-1 in HTR-8/SVneo cells; if yes, what is its function? We used HTR-8/SVneo cells, developed from ?rst trimester villous explants of early pregnancy, as the model of EVTs. MCP-1 gene silencing was performed using gene-specific siRNA. qPCR and ELISA were performed to estimate the expression and secretion of MCP-1. Here, we found that thrombin enhanced the secretion of MCP-1 in HTR-8/SVneo cells. Proteinase-activated receptor-1 (PAR-1) was found as the primary receptor, regulating MCP-1 secretion in these cells. Furthermore, MCP-1 secretion is modulated via protein kinase C (PKC) α, β, and Rho/Rho-kinase-dependent pathways. Thrombin negatively regulates HTR-8/SVneo cells’ ability to mimic tube formation in an MCP-1 dependent manner. In conclusion, we propose that thrombin-controlled MCP-1 secretion may play an essential role in normal placental development and successful pregnancy maintenance. Improper thrombin production and MCP-1 secretion during pregnancy might cause inadequate vascular formation and transformation of spiral arteries, which may contribute to pregnancy disorders, such as preeclampsia. 相似文献
986.
Nowacka-Woszuk J Cieslak J Skowronska B Majewska KA Stankiewicz W Fichna P Switonski M 《Journal of applied genetics》2011,52(3):319-323
Extensive studies of the MC4R gene polymorphism showed that, among numerous variants, there are mutations responsible for monogenic obesity, as well as
polymorphisms negatively correlated with the risk of obesity. In this report, we present the first studies of the whole coding
sequence of the MC4R gene in 243 Polish obese children and adolescents (the mean relative body mass index [RBMI] was 163.6). In addition, 101
non-obese adults were also analyzed. Direct sequencing facilitated the identification of six missense (K73R, V103I, T112M,
S127L, M215L, and I251L) and one silent (c.756 C > T) single-nucleotide polymorphisms (SNPs). Two non-synonymous polymorphisms
(K73R and M215L) appeared to be novel and one was found in obese patients (M215L, one patient) and one in non-obese adults
(K73R, one person). The overall frequency of non-synonymous variant carriers reached 4.1% and 6.9% in obese patients and non-obese
adults, respectively. Only one obesity-associated variant (127L) was found in two obese patients (0.82%) and in two non-obese
adults (1.98%). The obesity-protecting variants (103I and 251L) appeared to be the most common in both groups: 3.3% and 4.0%,
respectively. It was also observed that the RBMI in obese children and adolescents carrying the minor variants did not differ
significantly from the non-carriers; however, the expected trends for the associated and protecting variants were observed.
We conclude that the contribution of the MC4R gene variants to the pathogenesis of obesity in Polish children and adolescents is low. 相似文献
987.
Kurowska M Daszkowska-Golec A Gruszka D Marzec M Szurman M Szarejko I Maluszynski M 《Journal of applied genetics》2011,52(4):371-390
Recent advances in large-scale genome sequencing projects have opened up new possibilities for the application of conventional
mutation techniques in not only forward but also reverse genetics strategies. TILLING (Targeting Induced Local Lesions IN
Genomes) was developed a decade ago as an alternative to insertional mutagenesis. It takes advantage of classical mutagenesis,
sequence availability and high-throughput screening for nucleotide polymorphisms in a targeted sequence. The main advantage
of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of its genome size and ploidy
level. The TILLING protocol provides a high frequency of point mutations distributed randomly in the genome. The great mutagenic
potential of chemical agents to generate a high rate of nucleotide substitutions has been proven by the high density of mutations
reported for TILLING populations in various plant species. For most of them, the analysis of several genes revealed 1 mutation/200–500 kb
screened and much higher densities were observed for polyploid species, such as wheat. High-throughput TILLING permits the
rapid and low-cost discovery of new alleles that are induced in plants. Several research centres have established a TILLING
public service for various plant species. The recent trends in TILLING procedures rely on the diversification of bioinformatic
tools, new methods of mutation detection, including mismatch-specific and sensitive endonucleases, but also various alternatives
for LI-COR screening and single nucleotide polymorphism (SNP) discovery using next-generation sequencing technologies. The
TILLING strategy has found numerous applications in functional genomics. Additionally, wide applications of this throughput
method in basic and applied research have already been implemented through modifications of the original TILLING strategy,
such as Ecotilling or Deletion TILLING. 相似文献
988.
989.
Schaaf G Dynowski M Mousley CJ Shah SD Yuan P Winklbauer EM de Campos MK Trettin K Quinones MC Smirnova TI Yanagisawa LL Ortlund EA Bankaitis VA 《Molecular biology of the cell》2011,22(6):892-905
Sec14-superfamily proteins integrate the lipid metabolome with phosphoinositide synthesis and signaling via primed presentation of phosphatidylinositol (PtdIns) to PtdIns kinases. Sec14 action as a PtdIns-presentation scaffold requires heterotypic exchange of phosphatidylcholine (PtdCho) for PtdIns, or vice versa, in a poorly understood progression of regulated conformational transitions. We identify mutations that confer Sec14-like activities to a functionally inert pseudo-Sec14 (Sfh1), which seemingly conserves all of the structural requirements for Sec14 function. Unexpectedly, the "activation" phenotype results from alteration of residues conserved between Sfh1 and Sec14. Using biochemical and biophysical, structural, and computational approaches, we find the activation mechanism reconfigures atomic interactions between amino acid side chains and internal water in an unusual hydrophilic microenvironment within the hydrophobic Sfh1 ligand-binding cavity. These altered dynamics reconstitute a functional "gating module" that propagates conformational energy from within the hydrophobic pocket to the helical unit that gates pocket access. The net effect is enhanced rates of phospholipid-cycling into and out of the Sfh1* hydrophobic pocket. Taken together, the directed evolution approach reveals an unexpectedly flexible functional engineering of a Sec14-like PtdIns transfer protein-an engineering invisible to standard bioinformatic, crystallographic, and rational mutagenesis approaches. 相似文献
990.