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排序方式: 共有75条查询结果,搜索用时 15 毫秒
21.
Terasaki K Noda Y Shibahara T Itagaki T Fukuda K Tsuchiya K 《Parasitology international》2003,52(2):147-154
Experimental infection with Fasciola hepatica and parthenogenetic Fasciola sp. in laboratory animals have been conducted in rats and rabbits. Inoculation of less than 5 metacercariae into rat-like hamsters, Tscherskia triton, is sufficient to establish Fasciola infections. The prepatent period of F. hepatica and the parthenogenetic Fasciola sp. in T. triton was shorter than that in rats and rabbits, suggesting that T. triton is a suitable experimental model for these flukes. In contrast, F. gigantica infection in T. triton did not yield adult flukes; T. triton, is therefore, considered to be an unsuitable host for F. gigantica. The cotton rat, Sigmodon hispidus, was an unsuitable host for the parthenogenetic Fasciola sp. 相似文献
22.
Department of Biology, Faculty of Science, Okayama University,3-1-1, Tsushima-naka, Okayama, 700-8530 Japan In order to analyzethe influence of amino acid substitutions at the carboxyl-terminalprocessing site of the D1 precursor protein, mixed-culture growthexperiments were conducted for psbA directed mutants of Chlamydomonasreinhardtii. Wild type and D1 mutants were mixed in the sameculture and their viability was compared. Replacement of Ser-345by Gly or Val at the cleavage site markedly affected the relativegrowth rate of the mutant in the high intensity light, but notin a dim light or the darkness. This was consistent with theprevious result obtained by in vitro analysis using substitutedcarboxyl-terminal oligopeptides as substrates [Taguchi et al.(1995) J Biol. Chem. 270: 10711], This is a clear indicationthat the rate of carboxyl-terminal processing of the D1 precursorin the photosystem II reaction center is a rate-limiting stepfor growth under some environmental stress conditions. (Received June 9, 1998; Accepted September 25, 1998) 相似文献
23.
Narusaka Yoshihiro; Narusaka Mari; Kobayashi Hirokazu; Satoh Kimiyuki 《Plant & cell physiology》1998,39(6):620-626
Random mutations were introduced into the DNA fragment of thepsbA2 gene of Synechocystis sp. PCC 6803, which encodes thecarboxyl-terminal 178 amino acid region of the Dl protein ofthe PSII reaction center, by in vitro random mutagenesis toobtain Dl species resistant to herbicides and to understandthe protein-herbicide interactions. The mutants were screenedon the criterion of resistance to either 1 µM DCMU or10 µM atrazine. In these mutants, amino acid substitutionswere distributed throughout the entire area of the targetedregion in the Dl protein. However, in every mutant, except forone case, the substitution was present in the region describedas the "herbicide-binding niche", i.e., between Phe211 and Leu275,although some amino acid substitutions which were not previouslydescribed were found at residues known to be involved with herbicideaffinity. Thus, the result of random mutagenesis basically supportsthe validity of the proposed structural model for the Dl protein,as well as of the herbicide-binding niche. Preliminary characterizationof the herbicide-resistant mutants obtained in this study hasalso been conducted. (Received December 8, 1997; ) 相似文献
24.
Syusaku Suzuki Hiroharu Mifune Ryouzou Kamimura Akira Yabuki Tohol Obara Mitsuharu Matsumoto Kimiyuki Tsuchiya 《Experimental Animals》2003,52(5):441-444
The parotid and mandibular glands of the cotton rat were examined by light and transmission electron microscopy. Parotid gland: Acinar cells were serous in nature, and contained electron-dense granules. Intercalated duct cells contained electron-dense granules. Striated duct cells had small granules of moderate and high electron densities. Mandibular gland: Acinar cells were seromucous in nature, and contained granules of low and moderate electron densities. Intercalated duct cells contained granules of moderate and high electron densities. Striated ducts were comprised of two portions - a secretory portion and a striated portion without granules. The secretory portion had many electron-dense granules. A sexual dimorphism was obserbed in these granules, which were smaller and fewer in females than in males. 相似文献
25.
A part of mouse Zfy-2 sequence was synthesized and used to screen a genomic library of the spinous country-rat (Tokudaia osimensis spp., 2n = 45). An isolated clone had the C-terminal region of Zfy, which consisted of 1190 bp, encoded 336 amino acid residues, and harbored 11 out of 13 zinc finger motifs. With this as a
probe, a bovine testis cDNA library was screened. Two ZFX clones were isolated and their sequences combined. The short sequence,
lacking part of the 5′ upstream region, was amplified by PCR or RT-PCR, cloned, and sequenced. A full-length ZFX was constructed
by combining these three sequences. The bovine ZFX consisted of 5328 bp and encoded 800 amino acid residues, which contained 13 zinc finger motifs. ZFX was used as a probe for fluorescence in situ hybridization and was mapped to Xq34, different from its previously reported
site at Xq21-q231. A SINE (short interspersed nuclear element) sequence consisting of 188 bp was found close to the end of
the 3′-untranslated region of ZFX. The SINE sequence hybridized to all bovine chromosomes. ZFY is highly homologous with ZFX and, as a result, ZFY could be mapped simultaneously. ZFY was mapped to the distal region of the short arm of the Y Chromosome (Chr) (Yp13), contradicting the previously reported
position Yq1. Ovine and caprine ZFY were also mapped with bovine ZFX. Both were mapped to the distal region of the short arm of the Y Chr (Yp12-p13). Ovine ZFX was mapped to a region close to the centromere of the X Chr (Xq13).
Received: 23 July 1997 / Accepted: 30 September 1997 相似文献
26.
Hitoshi Suzuki Kimiyuki Tsuchiya Mitsuru Sakaizumi Shigeharu Wakana Osamu Gotoh Naruya Saitou Kazuo Moriwaki Susumu Sakurai 《Biochemical genetics》1990,28(3-4):137-149
Southern blot analysis of ribosomal DNA (rDNA) from seven species ofApodemus was carried out in order to examine the genetic relationships between the species. Analysis of heterogeneity in rDNA spacers inA. sylvaticus, A. flavicollis, A. semotus, A. agrarius, A. argenteus, A. speciosus, andA. peninsulae, using 13 different restriction enzymes and cloned mouse rDNA probes, revealed that the families of rDNA in these species can be characterized by restriction maps which show the major constituents of rDNA repeating units (repetypes). Based on differences in the arrangement of restriction sites, sequence divergence among the different major repetypes was estimated. Among the seven species ofApodemus examined, the major repetypes ofA. flavicollis andA. sylvaticus were the most closely related, having only 1.0% sequence divergence. These repetypes and those of the remaining five species differ substantially from one another, with 4.3–8.5% divergence.This study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan. 相似文献
27.
Takeda A Tsukuda M Mizumoto H Okamoto K Kaido M Mise K Okuno T 《The EMBO journal》2005,24(17):3147-3157
28.
Terasaki K Noda Y Itagaki T Shibahara T Tsuchiya K Fukuda K Inoue Y Iwamura M Hino A 《The Journal of parasitology》2002,88(5):1035-1037
Metacercariae of parthenogenetic Fasciola sp. triploid were inoculated into the rat-like hamster Tscherskia triton. Flukes at various stages of growth were found in the bile ducts of all 8 (50%) animals that survived from 42 to 90 days. The body length to width ratio ranged from 1.8 to 2.9, and flukes with the highest ratio were passed 68 days after inoculation. Our results indicate that T. triton is a suitable host for experimental infection when induced by a small number of metacercariae (less than 5) of Fasciola sp. 相似文献
29.
Mohammad Mahdi Najafpour Mohsen Abbasi Isaloo Julian J. Eaton-Rye Tatsuya Tomo Hiroshi Nishihara Kimiyuki Satoh Robert Carpentier Jian-Ren Shen Suleyman I. Allakhverdiev 《BBA》2014
The water-oxidizing complex (WOC), also known as the oxygen-evolving complex (OEC), of photosystem II in oxygenic photosynthetic organisms efficiently catalyzes water oxidation. It is, therefore, responsible for the presence of oxygen in the Earth's atmosphere. The WOC is a manganese–calcium (Mn4CaO5(H2O)4) cluster housed in a protein complex. In this review, we focus on water exchange chemistry of metal hydrates and discuss the mechanisms and factors affecting this chemical process. Further, water exchange rates for both the biological cofactor and synthetic manganese water splitting are discussed. The importance of fully unveiling the water exchange mechanism to understand the chemistry of water oxidation is also emphasized here. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy. 相似文献
30.
Jun J. Sato Akio Shinohara Nobumoto Miyashita Chihiro Koshimoto Kimiyuki Tsuchiya Ikuyo Nakahara Tetsuo Morita Hiromichi Yonekawa Kazuo Moriwaki Yasunori Yamaguchi 《Mammalian genome》2008,19(3):155-162
Genetic characterization of a wild-derived house mouse, Mus musculus, originally collected near Lake Balkhash in the Republic of Kazakhstan, was performed by examining protein polymorphisms
and nucleotide sequences for the hemoglobin beta chain (HBB) subunits. Protein electrophoresis, which was performed on a cellulose-acetate
plate, showed an independent mobility pattern representing a new, previously undiscovered haplotype. Neighbor-joining analyses
of the HBB adult genes, i.e., HBB-T1 and HBB-T2, and the intergenic spacer region showed that the Lake Balkhash mouse possessed
genomic components that were mixed from different haplotypes. Compared to the previously determined HBB haplotypes, d, p, and w1, the HBB-T1 gene and ca. 11 kb of the spacer region were most similar to the w1 haplotype; however, the remainder of the spacer region and the HBB-T2 gene were most similar to the d haplotype but may represent a still uncharacterized and divergent haplotype. The recombination event is predicted to have
occurred 2.5 kb upstream of the HBB-T2 gene and may have occurred through intersubspecific hybridization between mice of the
musculus subspecies group (with the w1 haplotype) and the castaneus subspecies group (with the d-like haplotype). Alternatively, an unknown subspecies group that is equidistantly divergent from each of these subspecies
groups may have been involved. Our findings suggest reticulate evolution among the subspecies groups during the evolution
of M. musculus. 相似文献