全文获取类型
收费全文 | 504篇 |
免费 | 30篇 |
出版年
2022年 | 2篇 |
2021年 | 2篇 |
2020年 | 3篇 |
2019年 | 5篇 |
2018年 | 4篇 |
2017年 | 10篇 |
2016年 | 12篇 |
2015年 | 15篇 |
2014年 | 14篇 |
2013年 | 35篇 |
2012年 | 32篇 |
2011年 | 31篇 |
2010年 | 21篇 |
2009年 | 18篇 |
2008年 | 27篇 |
2007年 | 39篇 |
2006年 | 34篇 |
2005年 | 33篇 |
2004年 | 30篇 |
2003年 | 43篇 |
2002年 | 26篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1998年 | 5篇 |
1997年 | 5篇 |
1996年 | 3篇 |
1995年 | 4篇 |
1994年 | 9篇 |
1993年 | 8篇 |
1992年 | 2篇 |
1991年 | 5篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1974年 | 5篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1969年 | 1篇 |
排序方式: 共有534条查询结果,搜索用时 15 毫秒
421.
Kimiko Okabe 《Experimental & applied acarology》1999,23(8):653-658
A bioassay was developed to quantify germination rates of Hypocrea nigricans spores passing through the alimentary canals of the three acarid mite species Tyrophagus putrescentiae, Histiogaster sp. and Schwiebea sp. These mites are suspected of causing damage to mushroom production in Japan. All three species feed on the fungus and excrete fungal spores and fungal particles. Fecal material of the mites was plated on malt agar plates supplemented with 1% CuSO4 to assess spore germination. Germination rates, and hence the possibility for the mites to disseminate spores of H. nigricans, was so high that these mites may indeed be important vectors of weed fungal spores in indoor mushroom production units. 相似文献
422.
Relationship between serum HDL-C levels and common genetic variants of the endothelial lipase gene in Japanese school-aged children 总被引:4,自引:0,他引:4
Endothelial lipase (EL) is a new member of the triglyceride lipase family, the genes of which play a central role in dietary fat absorption, energy homeostasis, and plasma lipoprotein metabolism. One physiologic role of EL is thought to be hydrolysis of high-density lipoprotein (HDL) phospholipid, although the precise function of endothelial lipase has yet to be fully clarified. Furthermore, genetic variation in EL has been suggested to influence serum HDL-C levels. In the present study, we detected two common single nucleotide polymorphisms in the EL gene associated with serum HDL cholesterol levels in healthy school-aged children. Our data support the hypothesis that variations in the EL gene are one of the genetic determinants of serum HDL-C levels. 相似文献
423.
Motohiro Hasegawa Kimiko Okabe Kenji Fukuyama Shun’ichi Makino Isamu Okochi Hiroshi Tanaka Hideaki Goto Takeo Mizoguchi Tadashi Sakata 《Experimental & applied acarology》2013,59(4):391-408
The community structures of Mesostigmata, Prostigmata, and Oribatida in the soil of broad-leaved regeneration forests and conifer plantations of various ages were assessed alongside soil and plant environmental variables using three response metrics (density, species richness, and species–abundance distribution). The density and species richness of mites recovered swiftly after clear-cutting or replanting. Oribatid mites dominated the soil mite communities in terms of densities and species richness for both forest types. Soil mite communities in broad-leaved forests was related to forest age, the crown tree communities index, and forest-floor litter weight. In contrast, soil mite communities in the conifer plantation sites were related to various indices of understory plants. The development of the understory plants was synchronized with the silvicultural schedules, including a closed canopy and thinning. Such a conifer plantation management may affect indirectly the community of mites. 相似文献
424.
Kimiko Uchii Hideyuki Doi Hiroki Yamanaka Toshifumi Minamoto 《Ecology and evolution》2017,7(20):8515-8522
Understanding behavioral differences between intraspecific genotypes of aquatic animals is challenging because we cannot directly observe the animals underwater or visually distinguish morphologically similar counterparts. Here, we tested a new monitoring tool that uses environmental DNA (eDNA), an assemblage of DNA in environmental water, to specifically detect Japanese native and introduced non‐native genotypes of common carp (Cyprinus carpio) in Lake Biwa, Japan, and estimated differences between the two genotypes in the use of inland habitats. We monitored the ratios of native and non‐native single nucleotide polymorphism alleles of a mitochondrial locus of common carp in a lagoon connected to Lake Biwa for 3 years using eDNA. We observed seasonal dynamics in the allele frequency showing that the native genotype frequency peaked every spring, suggesting that native individuals migrated to the lagoon for spawning and then returned to the main lake, whereas non‐native individuals tended to stay in the lagoon. The estimated migration patterns corresponded with the estimates of a previous study, which were based on commercial fish catch data. Our findings suggest that eDNA‐based monitoring can be useful tool for addressing intraspecific behavioral differences underwater. 相似文献
425.
Kimiko Okabe Motohiro Hasegawa Hiroshi Makihara 《Journal of Insect Conservation》2017,21(5-6):771-779
Characterising geographic patterns of biodiversity generated by intrinsic distributions of organisms is essential for designing effective biodiversity conservation plans on both the project and sub-national to national scales. Species composition is generally similar within the same types of ecosystems located close to one another, but similarity also depends on the focal organism(s) as well as the scale of the analysis. To facilitate decision-making for environmental compensation projects such as “biodiversity offsets”, we examined whether Japanese ecoregions based on vegetation are correlated with the distribution of cerambycid beetles, using existing cerambycid data collected from Hokkaido to the Nansei Islands in both natural and plantation forests over 1 year in each area. At the national level, the species compositions of beetles were quite distinct in Hokkaido and the Nansei Islands but less so in other areas. The overall pattern of the observed sampling data fit that obtained by previously accumulated local inventories. At the area level (including plantation forests under different management regimes/successional stages and in some cases naturally regenerated mature to old growth forests), no consistent beetle composition patterns were observed, although compositions in natural forests and closed canopy/thinned/old growth plantation forests were sometimes distinct. Therefore, we conclude that when ecoregions are considered during decision-making for “no net-loss or net-gain” biodiversity conservation measures, it is important to examine multiple organisms on various scales using scientific approaches from several perspectives. 相似文献
426.
427.
428.
Kazuhide KAWAZOE Kimiko OKABE Atsushi KAWAKITA Makoto KATO 《Entomological Science》2010,13(3):303-310
Since 2006, an introduced Oriental bamboo‐nesting large carpenter bee, Xylocopa tranquebarorum, has been recorded from the central Honshu Island, Japan, which is inhabited only by the endemic subspecies, Xylocopa appendiculata circumvolans. Carpenter bees (tribes Xylocopini and Ceratinini) have ecological associations with specific Sennertia spp. in all geographic regions of their distribution, thus it is worried that the introduced carpenter bee has brought non‐indigenous mites into Japan. In their native ranges, X. a. circumvolans and X. tranquebarorum each has specific Sennertia mite faunas: the four Japanese Alloxylocopa bees including X. a. circumvolans have associations with S. alfkeni, while X. tranquebarorum has association with S. potanini in China (except Taiwan) and with S. horrida in South to East Asia including Taiwan. In the present study, we examined phoretic mite fauna on the introduced X. tranquebarorum, and determined whether the mites are indigenous or not based on morphological character and two gene sequences (mitochondrial cytochrome oxidase subunit I gene and nuclear ribosomal internal transcribed spacer). It was found from the result of this study that the non‐indigenous Sennertia mite has invaded Japan with the introduced X. tranquebarorum. We discuss geographic origin of the introduced X. tranquebarorum based on associated mite fauna and potential ecological risk caused by the introduced Xylocopa‐Sennertia association. 相似文献
429.
Vascular functions are regulated not only by chemical mediators, such as hormones, cytokines, and neurotransmitters, but by
mechanical hemodynamic forces generated by blood flow and blood pressure. The mechanical force-mediated regulation is based
on the ability of vascular cells, including endothelial cells and smooth muscle cells, to recognize fluid mechanical forces,
i.e., the shear stress produced by flowing blood and the cyclic strain generated by blood pressure, and to transmit the signals
into the cell interior, where they trigger cell responses that involve changes in cell morphology, cell function, and gene
expression. Recent studies have revealed that immature cells, such as endothelial progenitor cells (EPCs) and embryonic stem
(ES) cells, as well as adult vascular cells, respond to fluid mechanical forces. Shear stress and cyclic strain promote the
proliferation and differentiation of EPCs and ES cells into vascular cells and enhance their ability to form new vessels.
Even more recently, attempts have been made to apply fluid mechanical forces to EPCs and ES cells cultured on polymer tubes
and develop tissue-engineered blood vessel grafts that have a structure and function similar to that of blood vessels in vivo.
This review summarizes the current state of knowledge concerning the mechanobiological responses of stem/progenitor cells
and its potential applications to tissue engineering. 相似文献
430.
Nakamura M Mie M Funabashi H Yamamoto K Ando J Kobatake E 《Analytical biochemistry》2006,352(1):61-67
We demonstrate the application of an assay technique for the detection of ATP efflux from the cell surface. Until recently, the firefly luciferase assay has conventionally been used to detect ATP release because of its high sensitivity and wide detection ability. However, since this technique can be used only to infer the amount of diffused ATP in bulk solutions, it does not accurately reflect the dynamic ATP flux at the cell membrane. We therefore constructed luciferase fusion proteins that could be immobilized onto the cell surface. However, the low activities and limited application ranges of these proteins prompted us to use biotinylated luciferase given its attributes of strong and stable localization. Furthermore, luciferase can be immobilized strongly onto the biotinylated cell surface via streptavidin-biotin binding and can thus be used to determine the dynamic release of ATP near the cell surface. 相似文献