Total nitrogen deposition at key growing stages of maize and wheat as affected by pot surface area and crop variety

Atmospheric nitrogen (N) deposition is a serious problem on the North China Plain (NCP) because it imposes a considerable nutrient burden on the local environment. However, it also makes a substantial contribution to agricultural crop N requirements. The integrated total N input (ITNI) system is a method to quantify total atmospheric N deposition by using ¹⁵N-labeled monitor plants grown in pots. The effect of pot surface area and variety of indicator plant on the amount of airborne N input quantified by the ITNI system was investigated in this study. Total N deposition to the soil-maize/soil-wheat plant system at key growth stages was also quantified to improve N-fertilizer recommendations. When indicator plants having a high space requirement were used a correction factor was needed and this could be obtained only by simulating commercial field conditions, especially plant density, because the factor depends largely on pot area or the difference in plant density between pot conditions and field conditions. The total airborne N input measured by the ITNI system was not influenced by the variety of monitoring plant. N deposition was 20?C25 kg N ha^?1 during growth from three expanded leaf to ten expanded leaf and also from ten expanded leaf to maturity of maize. N deposition was 29.1 kg N ha^?1 between planting and the jointing stage and 10.1 kg N ha^?1 from jointing to maturity of wheat. This high measured N deposition indicates that N deposition should be taken into account when calculating the N fertilizer requirements of maize and wheat in this region.     

Crop nitrogen use and soil mineral nitrogen accumulation under different crop combinations and patterns of strip intercropping in northwest China

Increasing crop nitrogen use efficiency while also simultaneously decreasing nitrogen accumulation in the soil would be key steps in controlling nitrogen pollution from agricultural systems. Long-term field experiments were started in 2003 to study the effects of intercropping on crop N use and soil mineral N accumulation in wheat (Triticum aestivum L. cv 2014)/maize (Zea mays L. cv Shendan16), wheat/faba bean (Vicia faba L. cv Lincan No. 5) and maize/faba bean intercropping and monocropping systems. Monocropping was compared with two types of strip intercropping: continuous intercropping (two crops intercropped continuously on the same strips of land every year) and rotational intercropping (two crops grown adjacently and rotated to the other crop??s strip every year). Maize/faba bean intercropping had greater crop N uptake than did wheat/faba bean or wheat/maize. Wheat/maize accumulated more mineral N in the top 140 cm of the soil profile during the co-growth stage from maize emergence to maturity of wheat or faba bean. Continuously intercropped maize substantially decreased soil mineral N accumulation under wheat and faba bean rows (60?C100 cm soil depth) at maize harvest. Soil mineral N accumulation under wheat rows increased with rotational intercropping with faba bean. Rotational intercropping may potentially alleviate the adverse effects of wheat on N use by other crops and increase the nitrogen harvest index of wheat, maize and faba bean. Intercropping using species with different maturity dates may be more effective in increasing crop N use efficiency and decreasing soil mineral N accumulation.     

Crustacean neuroendocrine systems and their signaling agents

Decapod crustaceans have long served as important models for the study of neuroendocrine signaling. For example, the process of neurosecretion was first formally demonstrated by using a member of this order. In this review, the major decapod neuroendocrine organs are described, as are their phylogenetic conservation and neurochemistry. In addition, recent advances in crustacean neurohormone discovery and tissue mapping are discussed, as are several recent advances in our understanding of hormonal control in this group of animals.     

Stomatin-like protein 2 binds cardiolipin and regulates mitochondrial biogenesis and function

Stomatin-like protein 2 (SLP-2) is a widely expressed mitochondrial inner membrane protein of unknown function. Here we show that human SLP-2 interacts with prohibitin-1 and -2 and binds to the mitochondrial membrane phospholipid cardiolipin. Upregulation of SLP-2 expression increases cardiolipin content and the formation of metabolically active mitochondrial membranes and induces mitochondrial biogenesis. In human T lymphocytes, these events correlate with increased complex I and II activities, increased intracellular ATP stores, and increased resistance to apoptosis through the intrinsic pathway, ultimately enhancing cellular responses. We propose that the function of SLP-2 is to recruit prohibitins to cardiolipin to form cardiolipin-enriched microdomains in which electron transport complexes are optimally assembled. Likely through the prohibitin functional interactome, SLP-2 then regulates mitochondrial biogenesis and function.     

Trafficking-deficient hERG K⁺ channels linked to long QT syndrome are regulated by a microtubule-dependent quality control compartment in the ER

The human ether-a-go-go related gene (hERG) encodes the voltage-gated K(+) channel that underlies the rapidly activating delayed-rectifier current in cardiac myocytes. hERG is synthesized in the endoplasmic reticulum (ER) as an "immature" N-linked glycoprotein and is terminally glycosylated in the Golgi apparatus. Most hERG missense mutations linked to long QT syndrome type 2 (LQT2) reduce the terminal glycosylation and functional expression. We tested the hypothesis that a distinct pre-Golgi compartment negatively regulates the trafficking of some LQT2 mutations to the Golgi apparatus. We found that treating cells in nocodazole, a microtubule depolymerizing agent, altered the subcellular localization, functional expression, and glycosylation of the LQT2 mutation G601S-hERG differently from wild-type hERG (WT-hERG). G601S-hERG quickly redistributed to peripheral compartments that partially colocalized with KDEL (Lys-Asp-Glu-Leu) chaperones but not calnexin, Sec31, or the ER golgi intermediate compartment (ERGIC). Treating cells in E-4031, a drug that increases the functional expression of G601S-hERG, prevented the accumulation of G601S-hERG to the peripheral compartments and increased G601S-hERG colocalization with the ERGIC. Coexpressing the temperature-sensitive mutant G protein from vesicular stomatitis virus, a mutant N-linked glycoprotein that is retained in the ER, showed it was not restricted to the same peripheral compartments as G601S-hERG at nonpermissive temperatures. We conclude that the trafficking of G601S-hERG is negatively regulated by a microtubule-dependent compartment within the ER. Identifying mechanisms that prevent the sorting or promote the release of LQT2 channels from this compartment may represent a novel therapeutic strategy for LQT2.     

Nitric oxide counteracts the hyperoxia-induced proliferation and proinflammatory responses of mouse astrocytes

Preclinical studies in the premature baboon evaluating the efficacy and potential toxicity of inhaled nitric oxide indicated a significant effect on astrocyte area density, suggesting phenotypic and functional changes in astrocytes upon exposure to nitric oxide. However, the effects of nitric oxide and oxygen, the two major therapeutic gases utilized in neonatal intensive care, on astrocyte morphology and function remain vastly unknown. Herein, we report that exposure of mouse neonatal cortical astrocytes to hyperoxia results in a proinflammatory phenotype and increase in proliferation without significant changes in cellular morphology or levels of intermediate filament proteins. The proinflammatory phenotype was evident by a significant increase in cellular levels of cyclooxygenase-2 and a concomitant increase in prostaglandin E₂ secretion, a decline in the intracellular and secreted levels of apolipoprotein E, and a significant increase in the intracellular levels of clusterin. This proinflammatory phenotype was not evident upon simultaneous exposure to hyperoxia and nitric oxide. These results suggest that exposure to nitric oxide in the setting of hyperoxia confers unrecognized beneficial effects by suppressing astrocytic inflammation.     

Design driven HtL: The discovery and synthesis of new high efficacy β₂-agonists

The design and synthesis of a new series of high efficacy β₂-agonists devoid of the key benzylic alcohol present in previously described highly efficacious β₂-agonists is reported. A hypothesis for the unprecedented level of efficacy is proposed based on considerations of β₂-adrenoceptor crystal structure, other biophysical data and modeling studies.     

Discovery of PH-797804, a highly selective and potent inhibitor of p38 MAP kinase

The synthesis and SAR studies of a novel N-aryl pyridinone class of p38 kinase inhibitors are described. Systematic structural modifications to the HTS lead, 5, led to the identification of (−)-4a as a clinical candidate for the treatment of inflammatory diseases. Additionally, the chiral synthesis and properties of (−)-4a are described.