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71.
Fungal infection of reproduction system of dairy cattle have not been received much more attention. The aim of this study
was to determine the fungal infection of Holstein dairy cows with reproductive disorders or healthy. Fungal isolates of cervicovaginal
fluids of 176 Holstein dairy cows were collected by using the double rod swabs for cervix and the sterilized cotton swabs
for discharges of vagina. They were evaluated for fungal infections. The treatment group included 70 dairy cows with reproductive
diseases, such as abortion, repeat breeder, endometritis, metritis, retention of fetal membrane, dystocia, cervicitis, and
vaginitis. The control groups were included 42 healthy non-pregnant cows and 64 pregnant cows. Isolates of fungi were obtained
from cervix and vagina of 27.1% and 28.6% of treatment group, 26.7% and 31.2% of pregnant cows, and 33.3% and 21.4% of healthy
non-pregnant cows, respectively, indicating no significant differences. It is showed that the cervix and vagina of the treatment
group have been infected by six different mycotic isolates. However, the cervix and vagina of pregnant and non-pregnant healthy
cows in control group were infected with 5, 6 and 5, 4 different fungal agents, respectively. Penicillium and yeast were the most common isolated agents. Regarding to the result of this study, it is concluded that fungal infections can occur
in cervicovaginal cavity of Holstein dairy cows with or without reproductive diseases. 相似文献
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ISABELLE LEFÈVRE KATARINA VOGEL‐MIKUŠ LUKA JEROMEL PRIMOŽ VAVPETIČ SÉBASTIEN PLANCHON IZTOK ARČON JOHANNES T VAN ELTEREN GILLES LEPOINT SYLVIE GOBERT JENNY RENAUT PRIMOŽ PELICON STANLEY LUTTS 《Plant, cell & environment》2014,37(6):1299-1320
Cadmium and zinc share many similar physiochemical properties, but their compartmentation, complexation and impact on other mineral element distribution in plant tissues may drastically differ. In this study, we address the impact of 10 μm Cd or 50 μm Zn treatments on ion distribution in leaves of a metallicolous population of the non‐hyperaccumulating species Zygophyllum fabago at tissue and cell level, and the consequences on the plant response through a combined physiological, proteomic and metabolite approach. Micro‐proton‐induced X‐ray emission and laser ablation inductively coupled mass spectrometry analyses indicated hot spots of Cd concentrations in the vicinity of vascular bundles in response to Cd treatment, essentially bound to S‐containing compounds as revealed by extended X‐ray absorption fine structure and non‐protein thiol compounds analyses. A preferential accumulation of Zn occurred in vascular bundle and spongy mesophyll in response to Zn treatment, and was mainly bound to O/N‐ligands. Leaf proteomics and physiological status evidenced a protection of photosynthetically active tissues and the maintenance of cell turgor through specific distribution and complexation of toxic ions, reallocation of some essential elements, synthesis of proteins involved in photosynthetic apparatus or C‐metabolism, and metabolite synthesis with some specificities regarding the considered heavy metal treatment. 相似文献
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