全文获取类型
收费全文 | 474篇 |
免费 | 23篇 |
出版年
2022年 | 4篇 |
2021年 | 6篇 |
2020年 | 3篇 |
2019年 | 3篇 |
2018年 | 7篇 |
2017年 | 6篇 |
2016年 | 11篇 |
2015年 | 19篇 |
2014年 | 20篇 |
2013年 | 28篇 |
2012年 | 36篇 |
2011年 | 38篇 |
2010年 | 17篇 |
2009年 | 14篇 |
2008年 | 21篇 |
2007年 | 22篇 |
2006年 | 18篇 |
2005年 | 12篇 |
2004年 | 11篇 |
2003年 | 21篇 |
2002年 | 14篇 |
2001年 | 16篇 |
2000年 | 17篇 |
1999年 | 16篇 |
1998年 | 5篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 9篇 |
1994年 | 8篇 |
1993年 | 5篇 |
1992年 | 5篇 |
1991年 | 5篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1985年 | 7篇 |
1984年 | 4篇 |
1982年 | 3篇 |
1980年 | 3篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1972年 | 4篇 |
1970年 | 6篇 |
1969年 | 2篇 |
1965年 | 2篇 |
1952年 | 2篇 |
排序方式: 共有497条查询结果,搜索用时 15 毫秒
461.
462.
463.
464.
Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2 总被引:19,自引:0,他引:19
下载免费PDF全文
![点击此处可从《Molecular biology of the cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Masckauchán TN Agalliu D Vorontchikhina M Ahn A Parmalee NL Li CM Khoo A Tycko B Brown AM Kitajewski J 《Molecular biology of the cell》2006,17(12):5163-5172
Wnts are lipid-modified secreted glycoproteins that regulate diverse biological processes. We report that Wnt5a, which functions in noncanonical Wnt signaling, has activity on endothelial cells. Wnt5a is endogenously expressed in human primary endothelial cells and is expressed in murine vasculature at several sites in mouse embryos and tissues. Expression of exogenous Wnt5a in human endothelial cells promoted angiogenesis. Wnt5a induced noncanonical Wnt signaling in endothelial cells, as measured by Dishevelled and ERK1/2 phosphorylation, and inhibition of canonical Wnt signaling, a known property of Wnt5a. Wnt5a induced endothelial cell proliferation and enhanced cell survival under serum-deprived conditions. The Wnt5a-mediated proliferation was blocked by Frizzled-4 extracellular domain. Wnt5a expression enhanced capillary-like network formation, whereas reduction of Wnt5a expression decreased network formation. Reduced Wnt5a expression inhibited endothelial cell migration. Screening for Wnt5a-regulated genes in cultured endothelial cells identified several encoding angiogenic regulators, including matrix metalloproteinase-1, an interstitial collagenase, and Tie-2, a receptor for angiopoietins. Thus, Wnt5a acts through noncanonical Wnt signaling to promote angiogenesis. 相似文献
465.
466.
467.
468.
EPR quantification of vascular nitric oxide production in genetically modified mouse models. 总被引:2,自引:0,他引:2
Jeffrey P Khoo Nicholas J Alp Jennifer K Bendall Seinosuke Kawashima Mitsuhiro Yokoyama Yin-Hua Zhang Barbara Casadei Keith M Channon 《Nitric oxide》2004,10(3):156-161
With increasing use of genetically modified mice to study endothelial nitric oxide (NO) biology, methods for reliable quantification of vascular NO production by mouse tissues are crucial. We describe a technique based on electron paramagnetic resonance (EPR) spectroscopy, using colloid iron (II) diethyldithiocarbamate [Fe(DETC)2], to trap NO. A signal was seen from C57BL/6 mice aortas incubated with Fe(DETC)2, that increased 4.7-fold on stimulation with calcium ionophore A23187 [3.45+/-0.13 vs 0.73+/-0.13au (arbitrary units)]. The signal increased linearly with incubation time (r(2) = 0.93), but was abolished by addition of N(G)-nitro-l-arginine methyl ester (L-NAME) or endothelial removal. Stimulated aortas from eNOS knockout mice had virtually undetectable signals (0.14+/-0.06 vs 3.17+/-0.21 au in littermate controls). However, the signal was doubled from mice with transgenic eNOS overexpression (7.17+/-0.76 vs 3.37+/-0.43 au in littermate controls). We conclude that EPR is a useful tool for direct NO quantification in mouse vessels. 相似文献
469.
A bioprocess for machine washable wool, combining the advantages of both protease and transglutaminase in a simultaneous enzymatic treatment has been developed. This process reduced the felting tendency of woven wool fabrics by 9% at the expense of only 2% weight and tensile strength loss. In contrast to previously described protease-based processes for shrink resistant wool, the anti-felting properties achieved in the simultaneous enzymatic treatment produced insignificant fibre damage, confirmed also by scanning electron images of the fabrics. 相似文献
470.
Lester Khoo David W. Robinette Edward J. Noga 《Marine biotechnology (New York, N.Y.)》1999,1(1):44-51
This paper describes the isolation of an approximately 3.7 kDa, basic, antibacterial peptide (designated callinectin), which
represents the major antibiotic activity in blue crab, Callinectes sapidus, hemocytes. A single-step purification using low-pressure cation-exchange chromatology yielded a highly purified (>95%) peptide.
Purity was confirmed by C4 reverse-phase high-performance liquid chromatography (RP-HPLC), native gel electrophoresis, sodium dodecyl sulfate–polyacrylamide
gel electrophoresis (SDS-PAGE), capillary electophoresis, and mass spectral analysis. The partial amino acid sequence obtained
via Edman degradation revealed no significant homology to other reported peptides in the Basic Local Alignment Search Tool
(BLAST) program database.
Received April 21, 1998; accepted July 3, 1998. 相似文献