排序方式: 共有84条查询结果,搜索用时 15 毫秒
81.
82.
Seyed Elyas Meshkani Narges Kooshan Arezoo Baradaran Moghadam Farahnaz Falanji Abolfazl Adli Fahimeh Baghbani-Arani Alireza Ghasemi Arian Abolfazl Rad 《Journal of cellular physiology》2019,234(10):18146-18155
Pterygium as a complex disease shares common features with other malignant cells in its onset recurrence and especially epithelial–mesenchymal transition (EMT) transition. Although using different approaches including conjunctival autografts, amniotic membrane, radiotherapy, mitomycin C (MMC) has shown promising insights in the inhibition of pterygium recurrence, it needs to be investigated in more details in molecular pathways to present adjuvant target therapy. In this study, we aimed to evaluate the expression of and then illustrate the role of signaling pathways on EMT in pterygium. Using real-time polymerase chain reaction, the twist-related protein 1 (TWIST1) expression was compared in primary pterygium and normal conjunctiva. This study assessed the mRNA expression, as well as the association between the clinicopathological indices and the gene expression level. The expression level of TWIST1 was overexpressed in 36% of our cohort ( n = 76). There was a significant positive correlation between recurrence with grade T, grade V and a significant negative correlation with growth activity. Our vast literature review on different signaling pathways in pterygium showed that EMT has centralization role in recurrence of this disease. Our data confirmed that EMT is important in the recurrence of pterygium samples and different signaling pathways end up activating the EMT markers. It is suggested to evaluate the environmental factors and their correlation with molecular markers to select favorable treatment for this kind of diseases. 相似文献
83.
84.
Aybike Birerdinc Rohini Mehta Reem Alhussain Arian Afendi Vikas Chandhoke Zobair Younossi Ancha Baranova 《Molecular Biology》2012,46(1):153-160
Real-time PCR (qRT-PCR) is the standard method for studying changes in relative gene expression in complex diseases like obesity
and gastritis. However, variations in amount of starting material, enzymatic efficiency and presence of amplification inhibitors
can lead to quantification errors. Hence, the need for accurate data normalization is vital. Among several known strategies
for data normalization, the use of reference genes as an internal control is the most common approach. Human gastric tissue
has been the least investigated for stability of reference gene expression. In this study, three popular algorithms, GeNorm, NormFinder and BestKeeper were used to evaluate the reference gene stability. Conclusion: HPRT1 and GAPDH are the best performing pair of reference
genes for qRT-PCR profiling experiments involving non-malignant gastric tissue samples. 相似文献