排序方式: 共有59条查询结果,搜索用时 62 毫秒
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Ghomi Khadijeh Rabiei Babak Sabouri Hossein Gholamalipour Alamdari Ebrahim 《Molecular biology reports》2021,48(10):6673-6694
Molecular Biology Reports - Barley is one of the major cereal crops, which can provide a significant source of genes for stress tolerance due to its high diversity and adaptability. Metabolite... 相似文献
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Khadijeh Khanaliha Mohammad Hossein Motazedian Bahram Kazemi Bahador Shahriari Mojgan Bandehpour Zarin Sharifniya 《The Korean journal of parasitology》2014,52(2):137-142
Serologic tests are widely accepted for diagnosing Toxoplasma gondii but purification and standardization of antigen needs to be improved. Recently, surface tachyzoite and bradyzoite antigens have become more attractive for this purpose. In this study, diagnostic usefulness of 3 recombinant antigens (SAG1, SAG2, and SAG3) were evaluated, and their efficacy was compared with the available commercial ELISA. The recombinant plasmids were transformed to JM109 strain of Escherichia coli, and the recombinants were expressed and purified. Recombinant SAG1, SAG2, and SAG3 antigens were evaluated using different groups of sera in an ELISA system, and the results were compared to those of a commercial IgG and IgM ELISA kit. The sensitivity and specificity of recombinant surface antigens for detection of anti-Toxoplasma IgG in comparison with commercially available ELISA were as follows: SAG1 (93.6% and 92.9%), SAG2 (100.0% and 89.4%), and SAG3 (95.4% and 91.2%), respectively. A high degree of agreement (96.9%) was observed between recombinant SAG2 and commercial ELISA in terms of detecting IgG anti-Toxoplasma antibodies. P22 had the best performance in detecting anti-Toxoplasma IgM in comparison with the other 2 recombinant antigens. Recombinant SAG1, SAG2, and SAG3 could all be used for diagnosis of IgG-specific antibodies against T. gondii. 相似文献
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The phonon and thermal properties of different single- ((n,0) (n = 7,8,9,10,14,15)) and double-walled carbon nanotubes ((7, 0) @ (14, 0), (8, 0) @ (14, 0), (9, 0) @ (15, 0) and (10, 0) @ (15, 0),) were calculated using the combination of density functional theory and non-equilibrium Green’s function methods. It was found that the Seebeck and Peltier coefficients for some of the single- and multi-walled carbon nanotubes have negative values. Moreover, in sharp contrast to low ?T, the higher thermoelectric figure of merit is anticipated at the higher temperature. The effect of the atoms number per unit cell on the phonons energies outweighs the effect of the vacuum and the size of the tubes for DWCNTs. All in all, the electron–phonon coupling generates the roughly plethora of thermoelectric coefficients and thermal conductance. 相似文献
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A comparison of the folding characteristics of free and ribosome-tethered polypeptide chains using limited proteolysis and mass spectrometry
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Khadijeh Rajabi Julia Reuther Elke Deuerling Sheena E Radford Alison E Ashcroft 《Protein science : a publication of the Protein Society》2015,24(8):1282-1291
The kinetics and thermodynamics of protein folding are commonly studied in vitro by denaturing/renaturing intact protein sequences. How these folding mechanisms relate to de novo folding that occurs as the nascent polypeptide emerges from the ribosome is much less well understood. Here, we have employed limited proteolysis followed by mass spectrometry analyses to compare directly free and ribosome-tethered polypeptide chains of the Src-homology 3 (SH3) domain and its unfolded variant, SH3-m10. The disordered variant was found to undergo faster proteolysis than SH3. Furthermore, the trypsin cleavage patterns observed show minor, but significant, differences for the free and ribosome-bound nascent chains, with significantly fewer tryptic peptides detected in the presence of ribosome. The results highlight the utility of limited proteolysis coupled with mass spectrometry for the structural analysis of these complex systems, and pave the way for detailed future analyses by combining this technique with chemical labeling methods (for example, hydrogen-deuterium exchange, photochemical oxidation) to analyze protein folding in real time, including in the presence of additional ribosome-associated factors. 相似文献
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