Plasmids pMB123 and pMB124--vectors for obtaining subfragments of DNA with random "sticky" ends

For preparing a DNA fragment with unique protruding ends, plasmid vectors pMB123 and pMB124 were constructed by inserting a synthetic polylinker into plasmid pUR222 at the EcoRI-PstI sites. The polylinker contains two FokI and HgaI sites at its ends in opposite orientation flanking a combination of SalGI, AccI, HindII, HindIII (the latter site is absent from pMB124) and BamHI sites. DNA fragment cloned at the SalGI and BamHI sites can be regenerated by either FokI or HgaI treatment, the SalGI and BamHI sites being deleted from the cloned sequence. Fragments coding for parts of human interleukin-2 were cloned in these vectors.     

Characteristics of higher nervous activity in the suslik Citellus fulvus during its active period

In experiments on active ground squirrels, it was possible to form motor defensive conditioned reflexes and to re-arrange the conditioned reflexes from visual and auditory analysers. It was originally demonstrated that repetitive alteration of the informational content of the conditioned stimuli results in neurotic disturbances in animals.     

Detection of the complex of chromatin DNase-tRNA by gel filtration

By gel filtration on Sephadex G-100, the formation of the complex of chromatin DNase-tRNA has been detected. The complex is reactivated after RNase treatment. The molecular weight of the enzyme-inhibitory complex is estimated to be 85,000.     

Therapeutic effect of the transfusion of UV-irradiated donor plasma in mechanical jaundice

The method of intravenous administration of UV-irradiated donor plasma to patients with jaundice of different genesis has been tested in the experiments on rats and used clinically in 20 patients. Biochemical and morphological studies have shown that such treatment causes stable improvement in the liver functional state.     

Changes in nerve structure on one side of the body following section of the inferior alveolar and sciatic nerves on the opposite side of the body in dogs]

The author describes changes of the lower alveolar and ischiadic nerves on the non-operated side after cutting such nerves on the opposite side of dogs, as well as changes in the organs innervated by them. The observed dystrophic changes in the nerve fibres on the non-operated side turn into more severe and non-reversible changes due to abnormal stimulation. Changes of masticatory organs appearing after cutting the lower alveolar nerve have bilateral manifestations being more pronounced on the same side especially in the case of a simultaneous dissection of the lower alveolar artery. These investigations contribute to possible interpretation of cases of symmetrical caries, development of dento-maxillary deformations on both sides.     

Effect of the limbic cortex on regional lymph and blood flow in dogs]

Electrical stimulation of the anterior limbic area of the cortex in dogs resulted in increase of the lymph flow from the intestinal lymph duct, in enlargement of the mesenteric lymph nodes, in a fall of arterial pressure in the cranial mesenteric artery, and in decrease of the blood-stream from the homonymous vein and in the tone of the mesenteric veins. Constriction of the mesenteric veins and increase in the intestinal lymph-stream are considered to be a compensatory reaction to restore the arterial pressure to the initial level.     

Neurotrophic control of the transmembrane Cl− pump in mammalian muscle fibers

Resting membrane potential of both innervated and denervated rat diaphragm muscle fibers was investigated when the concentration of potential-producing ions was changed in the external fluid and following treatment with furosemide. It was found that equilibrium potential ( ) equalled resting potential level in innervated muscle for Cl^–, but shifts to more positive values compared with resting membrane potential following section of the nerve. Furosemide retards development of post-denervation depolarization of the muscle membrane. It is deduced that trophic influences originating from the motor nerve activates the furosemide-sensitive Cl^– influx system, leading to raised intracellular concentration of Cl^–, a shift in (E_Cl) and depolarization of the muscle membrane.S. V. Kurashov Medical Institute, Minsitry of Health of the RSFSR, Kazan'. Translated from Neirofiziologiya, Vol. 19, No. 6, pp. 766–771, November–December, 1987.