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831.
Austin M. Harvey David G. Rehard Katie M. Groskreutz Danielle R. Kuntz Kevin J. Sharp Patrick K. T. Shiu Thomas M. Hammond 《Genetics》2014,197(4):1165-1174
Neurospora fungi harbor a group of meiotic drive elements known as Spore killers (Sk). Spore killer-2 (Sk-2) and Spore killer-3 (Sk-3) are two Sk elements that map to a region of suppressed recombination. Although this recombination block is limited to crosses between Sk and Sk-sensitive (SkS) strains, its existence has hindered Sk characterization. Here we report the circumvention of this obstacle by combining a classical genetic screen with next-generation sequencing technology and three-point crossing assays. This approach has allowed us to identify a novel locus called rfk-1, mutation of which disrupts spore killing by Sk-2. We have mapped rfk-1 to a 45-kb region near the right border of the Sk-2 element, a location that also harbors an 11-kb insertion (Sk-2INS1) and part of a >220-kb inversion (Sk-2INV1). These are the first two chromosome rearrangements to be formally identified in a Neurospora Sk element, providing evidence that they are at least partially responsible for Sk-based recombination suppression. Additionally, the proximity of these chromosome rearrangements to rfk-1 (a critical component of the spore-killing mechanism) suggests that they have played a key role in the evolution of meiotic drive in Neurospora. 相似文献
832.
The aim of the present study was to use the analysis of surface water chemistry to understand vegetation succession pathways in terrestrializing polyhumic lakes. We hypothesized that Sphagnum mire development was accompanied by a decrease in the mineral content in water. A total of 111 vegetation plots along 23 transects were analysed in 11 lakes and adjacent peat lands in the Wigry National Park (NE Poland). The vegetation of the lake-mire systems forms distinct zones: (1) nymphaeid-, bladderwort- and bryophyte-dominated aquatic vegetation; (2) sedge-dominated edge of the Sphagnumcarpet; (3) quaking, extremely poor fen with various Cyperaceae; (4) non-quaking, Eriophorum vaginatum-dominated bog-like vegetation and (5) pine woodland. Surface water corrected conductivity (ECcorr.), pH, COD-KMnO4 and Ca2+, Mg2+, Fetot. and SiO2 were measured along the transects. The environmental gradients best explaining the observed pattern were pH (with the highest values in the lake and the lowest in the bog-like vegetation) and COD-KMnO4 (showing an inverse direction). At least in some Sphagnum-mires conditions were more minerotrophic than in the lakes. The process of humic lake overgrowing by Sphagnum-mires in NE Poland results in pine woodlands on mineralised peat. The climate conditions in NE Poland, combined with evapotranspiration accelerated by encroaching trees, do not seem to support the development of ombrotrophic bogs. 相似文献
833.
Supplementary feeding of birds, particularly in urban areas, is often associated with increased population size and fecundity. In the UK, the non‐native Grey Squirrel Sciurus carolinensis is common in rural and urban habitats. It exploits supplementary feeders and may induce interference competition by excluding birds, but empirical evidence of this is unavailable. Using controlled model presentation experiments, we demonstrate that Grey Squirrels could reduce bird use of supplementary feeders and induce interference competition. Total bird resource use was reduced by 98% and most species exhibited similar sensitivities. The likelihood and magnitude of interference competition will depend on how rapidly displaced birds find alternative food sources; it will be greatest where there are high Grey Squirrel densities and few supplementary feeders. Other studies suggest that supplementary feeding increases Grey Squirrel numbers, and the species is also predicted to expand its non‐native range across most of Europe. Our data indicate that Grey Squirrels may eventually alter the net effect of supplementary feeding on bird populations across the European continent; increased use of squirrel‐proof feeders may help to minimize such effects. 相似文献
834.
Michael W. Hart Jennifer M. Sunday Iva Popovic Kevin J. Learning Christine M. Konrad 《Evolution; international journal of organic evolution》2014,68(5):1294-1305
Reproductive isolation—the key event in speciation—can evolve when sexual conflict causes selection favoring different combinations of male and female adaptations in different populations. Likely targets of such selection include genes that encode proteins on the surfaces of sperm and eggs, but no previous study has demonstrated intraspecific coevolution of interacting gamete recognition genes under selection. Here, we show that selection drives coevolution between an egg receptor for sperm (OBi1) and a sperm acrosomal protein (bindin) in diverging populations of a sea star (Patiria miniata). We found positive selection on OBi1 in an exon encoding part of its predicted substrate‐binding protein domain, the ligand for which is found in bindin. Gene flow was zero for the parts of bindin and OBi1 in which selection for high rates of amino acid substitution was detected; higher gene flow for other parts of the genome indicated selection against immigrant alleles at bindin and OBi1. Populations differed in allele frequencies at two key positively selected sites (one in each gene), and differences at those sites predicted fertilization rate variation among male–female pairs. These patterns suggest adaptively evolving loci that influence reproductive isolation between populations. 相似文献
835.
Seán S. óhéigeartaigh David Armisén Kevin P. Byrne Kenneth H. Wolfe 《Journal of bacteriology》2014,196(11):2030-2042
We report the development of SearchDOGS Bacteria, software to automatically detect missing genes in annotated bacterial genomes by combining BLAST searches with comparative genomics. Having successfully applied the approach to yeast genomes, we redeveloped SearchDOGS to function as a standalone, downloadable package, requiring only a set of GenBank annotation files as input. The software automatically generates a homology structure using reciprocal BLAST and a synteny-based method; this is followed by a scan of the entire genome of each species for unannotated genes. Results are provided in a HTML interface, providing coordinates, BLAST results, syntenic location, omega values (Ka/Ks, where Ks is the number of synonymous substitutions per synonymous site and Ka is the number of nonsynonymous substitutions per nonsynonymous site) for protein conservation estimates, and other information for each candidate gene. Using SearchDOGS Bacteria, we identified 155 gene candidates in the Shigella boydii sb227 genome, including 56 candidates of length < 60 codons. SearchDOGS Bacteria has two major advantages over currently available annotation software. First, it outperforms current methods in terms of sensitivity and is highly effective at identifying small or highly diverged genes. Second, as a freely downloadable package, it can be used with unpublished or confidential data. 相似文献
836.
Agata Rekas Robert B. Knott Anna Sokolova Kevin J. Barnham Keyla A. Perez Colin L. Masters Simon C. Drew Roberto Cappai Cyril C. Curtain Chi L. L. Pham 《European biophysics journal : EBJ》2010,39(10):1407-1419
Inclusions of aggregated α-synuclein (α-syn) in dopaminergic neurons are a characteristic histological marker of Parkinson’s
disease (PD). In vitro, α-syn in the presence of dopamine (DA) at physiological pH forms SDS-resistant non-amyloidogenic oligomers.
We used a combination of biophysical techniques, including sedimentation velocity analysis, small angle X-ray scattering (SAXS)
and circular dichroism spectroscopy to study the characteristics of α-syn oligomers formed in the presence of DA. Our SAXS
data show that the trimers formed by the action of DA on α-syn consist of overlapping worm-like monomers, with no end-to-end
associations. This lack of structure contrasts with the well-established, extensive β-sheet structure of the amyloid fibril
form of the protein and its pre-fibrillar oligomers. We propose on the basis of these and earlier data that oxidation of the
four methionine residues at the C- and N-terminal ends of α-syn molecules prevents their end-to-end association and stabilises
oligomers formed by cross linking with DA-quinone/DA-melanin, which are formed as a result of the redox process, thus inhibiting
formation of the β-sheet structure found in other pre-fibrillar forms of α-syn. 相似文献
837.
Gabriel O. Reznik Prashanth Vishwanath Michelle A. Pynn Joy M. Sitnik Jeffrey J. Todd Jun Wu Yan Jiang Brendan G. Keenan Andrew B. Castle Richard F. Haskell Temple F. Smith Ponisseril Somasundaran Kevin A. Jarrell 《Applied microbiology and biotechnology》2010,86(5):1387-1397
Surfactants find wide commercial use as foaming agents, emulsifiers, and dispersants. Currently, surfactants are produced from petroleum, or from seed oils such as palm or coconut oil. Due to concerns with CO2 emissions and the need to protect rainforests, there is a growing necessity to manufacture these chemicals using sustainable resources In this report, we describe the engineering of a native nonribosomal peptide synthetase pathway (i.e., surfactin synthetase), to generate a Bacillus strain that synthesizes a highly water-soluble acyl amino acid surfactant, rather than the water insoluble lipopeptide surfactin. This novel product has a lower CMC and higher water solubility than myristoyl glutamate, a commercial surfactant. This surfactant is produced by fermentation of cellulosic carbohydrate as feedstock. This method of surfactant production provides an approach to sustainable manufacturing of new surfactants. 相似文献
838.
839.
Shirley Chiang Tanya Burch Gary Van Domselaar Kevin Dick Alina Radziwon Craig Brusnyk Megan Rae Edwards Jessica Piper Todd Cutts Jingxin Cao Xuguang Li Runtao He 《Molecular and cellular biochemistry》2010,334(1-2):221-232
The cellular counterpart of the “soluble” guanylyl cyclase found in tissue homogenates over 30 years ago is now recognized as the physiological receptor for nitric oxide (NO). The ligand-binding site is a prosthetic haem group that, when occupied by NO, induces a conformational change in the protein that propagates to the catalytic site, triggering conversion of GTP into cGMP. This review focuses on recent research that takes this basic information forward to the beginnings of a quantitative depiction of NO signal transduction, analogous to that achieved for other major transmitters. At its foundation is an explicit enzyme-linked receptor mechanism for NO-activated guanylyl cyclase that replicates all its main properties. In cells, NO signal transduction is subject to additional, activity-dependent modifications, notably through receptor desensitization and changes in the activity of cGMP-hydrolyzing phosphodiesterases. The measurement of these parameters under varying conditions in rat platelets has made it possible to formulate a cellular model of NO-cGMP signaling. The model helps explain cellular responses to NO and their modification by therapeutic agents acting on the guanylyl cyclase or phosphodiesterase limbs of the pathway. 相似文献
840.
Stephanie J. Culler Kevin G. Hoff Rodger B. Voelker J. Andrew Berglund Christina D. Smolke 《Nucleic acids research》2010,38(15):5152-5165
Despite the critical role of pre-mRNA splicing in generating proteomic diversity and regulating gene expression, the sequence composition and function of intronic splicing regulatory elements (ISREs) have not been well elucidated. Here, we employed a high-throughput in vivo Screening PLatform for Intronic Control Elements (SPLICE) to identify 125 unique ISRE sequences from a random nucleotide library in human cells. Bioinformatic analyses reveal consensus motifs that resemble splicing regulatory elements and binding sites for characterized splicing factors and that are enriched in the introns of naturally occurring spliced genes, supporting their biological relevance. In vivo characterization, including an RNAi silencing study, demonstrate that ISRE sequences can exhibit combinatorial regulatory activity and that multiple trans-acting factors are involved in the regulatory effect of a single ISRE. Our work provides an initial examination into the sequence characteristics and function of ISREs, providing an important contribution to the splicing code. 相似文献