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排序方式: 共有323条查询结果,搜索用时 26 毫秒
241.
Kuang Z Yao S McNeil KA Thompson JA Bach LA Forbes BE Wallace JC Norton RS 《Biochemistry》2007,46(48):13720-13732
A family of six insulin-like growth factor (IGF) binding proteins (IGFBP-1-6) binds IGF-I and IGF-II with high affinity and thus regulates their bioavailability and biological functions. IGFBPs consist of N- and C-terminal domains, which are highly conserved and cysteine-rich, joined by a variable linker domain. The role of the C-domain in IGF binding is not completely understood in that C-domain fragments have very low or even undetectable IGF binding affinity, but loss of the C-domain dramatically disrupts IGF binding by IGFBPs. We recently reported the solution structure and backbone dynamics of the C-domain of IGFBP-2 (C-BP-2) and identified a pH-dependent heparin binding site [Kuang, Z., Yao, S., Keizer, D. W., Wang, C. C., Bach, L. A., Forbes, B. E., Wallace, J. C., and Norton, R. S. (2006) Structure, dynamics and heparin binding of the C-terminal domain of insulin-like growth factor-binding protein-2 (IGFBP-2), J. Mol. Biol. 364, 690-704]. Here, we have analyzed the molecular interactions among the N-domain of IGFBP-2 (N-BP-2), C-BP-2, and IGFs using cross-linking and nuclear magnetic resonance (NMR) spectroscopy. The binding of C-BP-2 to the IGF-I.N-BP-2 binary complex was significantly stronger than the binding of C-BP-2 to IGF-I alone, switching from intermediate exchange to slow exchange on the NMR time scale. A conformational change or stabilization of the IGF-I Phe49-Leu54 region and the Phe49 aromatic ring upon binding to the N-domains, as well as an interdomain interaction between N-BP-2 and C-BP-2 (which is also detectable in the absence of ligand), may contribute to this cooperativity in IGF binding. Glycosaminoglycan binding by IGFBPs can affect their IGF binding although the effects appear to differ among different IGFBPs; here, we found that heparin bound to the IGF-I.N-BP-2.C-BP-2 ternary complex, but did not cause it to dissociate. 相似文献
242.
243.
Camilla Beate Hill Tefera Tolera Angessa Lee‐Anne McFawn Debbie Wong Josquin Tibbits Xiao‐Qi Zhang Kerrie Forrest David Moody Paul Telfer Sharon Westcott Dean Diepeveen Yanhao Xu Cong Tan Matthew Hayden Chengdao Li 《Plant biotechnology journal》2019,17(5):932-944
Barley (Hordeum vulgare L.) is a major cereal grain widely used for livestock feed, brewing malts and human food. Grain yield is the most important breeding target for genetic improvement and largely depends on optimal timing of flowering. Little is known about the allelic diversity of genes that underlie flowering time in domesticated barley, the genetic changes that have occurred during breeding, and their impact on yield and adaptation. Here, we report a comprehensive genomic assessment of a worldwide collection of 895 barley accessions based on the targeted resequencing of phenology genes. A versatile target‐capture method was used to detect genome‐wide polymorphisms in a panel of 174 flowering time‐related genes, chosen based on prior knowledge from barley, rice and Arabidopsis thaliana. Association studies identified novel polymorphisms that accounted for observed phenotypic variation in phenology and grain yield, and explained improvements in adaptation as a result of historical breeding of Australian barley cultivars. We found that 50% of genetic variants associated with grain yield, and 67% of the plant height variation was also associated with phenology. The precise identification of favourable alleles provides a genomic basis to improve barley yield traits and to enhance adaptation for specific production areas. 相似文献
244.
Julie M. Nicol Kerrie A. Davies Trevor W. Hancock John M. Fisher 《Journal of nematology》1999,31(4):367-376
A two-year field trial with 130 plots was conducted at Tanunda, South Australia. Ten cereal cultivars differing in susceptibility to Pratylenchus thornei, two poor host crops (non-leguminous), and a bare fallow treatment were used to manipulate the numbers of nematodes in the plots in the first year. Initial and final densities were determined for each plot and varied from 0 to 9,400 nematodes/200 g oven-dried soil at the beginning of the second year. A highly susceptible wheat cultivar, Warigal, and two wheat lines known to have some resistance to P. thornei, GS50A and AUS4930, were planted in the second year. High densities of P. thornei caused more extensive lesions and severe cortical degradation in roots of Warigal than in GS50A or AUS4930. There was a significant linear relationship between initial density of P. thornei and Warigal grain yield (t/ha), with the estimated regression equation Y = 1.86 - 0.0000557x, where Y is the grain yield in t/ha and x is the number of P. thornei/200 g oven-dried soil. High initial densities (9,000 P. thornei/200 g oven-dried soil) caused up to 27% yield loss of this commercial Australian wheat. In contrast, the yield of the two resistant lines was not affected by initial density, suggesting that both were tolerant as well as resistant in the field. 相似文献
245.
Narayana M. Upadhyaya Xue-Rong Zhou Limin Wu Kerrie Ramm Elizabeth S. Dennis 《Plant Molecular Biology Reporter》2000,18(3):227-233
A conditional negative selection marker is essential for high throughput insertional mutagenesis with any two-element transposon
tagging system. Thetms2 gene encodes indoleacetic acid hydrolase (IAAH) which converts naphthaleneacetamide (NAM) to the potent auxin naphthaleneacetic
acid, a phytotoxic derivative. This gene, under the control of the manopine synthase gene 2 promoter fromAgrobacterium tumefaciens and exogenously applied NAM, have been used effectively as a negative selector inAc/Ds insertional mutagenesis ofArabidopsis thaliana (Sundaresan et al., 1995). In this study we show thattms2 can also be used as a negative selector in rice. T1 transgenic seedlings expressing thistms2 gene under the control of themas2’ promoter showed significant reduction in shoot and root growth in the presence of 5–10 μM NAM under specified growth conditions
compared to plants not containing this gene. 相似文献
246.
Coleman NV Wilson NL Barry K Brettin TS Bruce DC Copeland A Dalin E Detter JC Del Rio TG Goodwin LA Hammon NM Han S Hauser LJ Israni S Kim E Kyrpides N Land ML Lapidus A Larimer FW Lucas S Pitluck S Richardson P Schmutz J Tapia R Thompson S Tice HN Spain JC Gossett JG Mattes TE 《Journal of bacteriology》2011,193(13):3399-3400
Nocardioides sp. strain JS614 grows on ethene and vinyl chloride (VC) as sole carbon and energy sources and is of interest for bioremediation and biocatalysis. Sequencing of the complete genome of JS614 provides insight into the genetic basis of alkene oxidation, supports ongoing research into the physiology and biochemistry of growth on ethene and VC, and provides biomarkers to facilitate detection of VC/ethene oxidizers in the environment. This is the first genome sequence from the genus Nocardioides and the first genome of a VC/ethene-oxidizing bacterium. 相似文献
247.
Robert King Stephen Nicol Paul Cramp Kerrie M. Swadling 《Marine and Freshwater Behaviour and Physiology》2013,45(4):271-283
Live Antarctic krill, Euphausia superba, have been maintained for experimental purposes at the Australian Antarctic Division since 1981. This population has been replenished on an annual basis with animals taken from the wild. Techniques used to capture and maintain live krill are discussed here, with particular reference given to the development of systems for their maintenance. Details are also provided for specific experimental systems that have been used to conduct research into the behaviour and physiology of krill both at-sea and in shore-based laboratories. 相似文献
248.
Wu Weihua Davis Ryan W. Tran-Gyamfi Mary Bao Kuo Alan LaButti Kurt Mihaltcheva Sirma Hundley Hope Chovatia Mansi Lindquist Erika Barry Kerrie Grigoriev Igor V. Henrissat Bernard Gladden John M. 《Applied microbiology and biotechnology》2017,101(6):2603-2618
Applied Microbiology and Biotechnology - Recently, several endophytic fungi have been demonstrated to produce volatile organic compounds (VOCs) with properties similar to fossil fuels, called... 相似文献
249.
Raj K. Pasam Urmil Bansal Hans D. Daetwyler Kerrie L. Forrest Debbie Wong Joanna Petkowski Nicholas Willey Mandeep Randhawa Mumta Chhetri Hanif Miah Josquin Tibbits Harbans Bariana Matthew J. Hayden 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(4):777-793
Key message
BayesR and MLM association mapping approaches in common wheat landraces were used to identify genomic regions conferring resistance to Yr, Lr, and Sr diseases.Abstract
Deployment of rust resistant cultivars is the most economically effective and environmentally friendly strategy to control rust diseases in wheat. However, the highly evolving nature of wheat rust pathogens demands continued identification, characterization, and transfer of new resistance alleles into new varieties to achieve durable rust control. In this study, we undertook genome-wide association studies (GWAS) using a mixed linear model (MLM) and the Bayesian multilocus method (BayesR) to identify QTL contributing to leaf rust (Lr), stem rust (Sr), and stripe rust (Yr) resistance. Our study included 676 pre-Green Revolution common wheat landrace accessions collected in the 1920–1930s by A.E. Watkins. We show that both methods produce similar results, although BayesR had reduced background signals, enabling clearer definition of QTL positions. For the three rust diseases, we found 5 (Lr), 14 (Yr), and 11 (Sr) SNPs significant in both methods above stringent false-discovery rate thresholds. Validation of marker–trait associations with known rust QTL from the literature and additional genotypic and phenotypic characterisation of biparental populations showed that the landraces harbour both previously mapped and potentially new genes for resistance to rust diseases. Our results demonstrate that pre-Green Revolution landraces provide a rich source of genes to increase genetic diversity for rust resistance to facilitate the development of wheat varieties with more durable rust resistance.250.
Fan Shi Josquin Tibbits Raj K. Pasam Pippa Kay Debbie Wong Joanna Petkowski Kerrie L. Forrest Ben J. Hayes Alina Akhunova John Davies Steven Webb German C. Spangenberg Eduard Akhunov Matthew J. Hayden Hans D. Daetwyler 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(7):1393-1404