全文获取类型
收费全文 | 818篇 |
免费 | 55篇 |
出版年
2023年 | 3篇 |
2022年 | 18篇 |
2021年 | 21篇 |
2020年 | 17篇 |
2019年 | 22篇 |
2018年 | 17篇 |
2017年 | 22篇 |
2016年 | 19篇 |
2015年 | 37篇 |
2014年 | 51篇 |
2013年 | 62篇 |
2012年 | 81篇 |
2011年 | 63篇 |
2010年 | 39篇 |
2009年 | 43篇 |
2008年 | 47篇 |
2007年 | 51篇 |
2006年 | 35篇 |
2005年 | 50篇 |
2004年 | 42篇 |
2003年 | 20篇 |
2002年 | 18篇 |
2001年 | 16篇 |
2000年 | 13篇 |
1999年 | 13篇 |
1998年 | 3篇 |
1997年 | 7篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 4篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 4篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 3篇 |
1978年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有873条查询结果,搜索用时 281 毫秒
171.
Benjamin A. Lange Christine Michel Justin F. Beckers J. Alec Casey Hauke Flores Ido Hatam Guillaume Meisterhans Andrea Niemi Christian Haas 《PloS one》2015,10(4)
With near-complete replacement of Arctic multi-year ice (MYI) by first-year ice (FYI) predicted to occur within this century, it remains uncertain how the loss of MYI will impact the abundance and distribution of sea ice associated algae. In this study we compare the chlorophyll a (chl a) concentrations and physical properties of MYI and FYI from the Lincoln Sea during 3 spring seasons (2010-2012). Cores were analysed for texture, salinity, and chl a. We identified annual growth layers for 7 of 11 MYI cores and found no significant differences in chl a concentration between the bottom first-year-ice portions of MYI, upper old-ice portions of MYI, and FYI cores. Overall, the maximum chl a concentrations were observed at the bottom of young FYI. However, there were no significant differences in chl a concentrations between MYI and FYI. This suggests little or no change in algal biomass with a shift from MYI to FYI and that the spatial extent and regional variability of refrozen leads and younger FYI will likely be key factors governing future changes in Arctic sea ice algal biomass. Bottom-integrated chl a concentrations showed negative logistic relationships with snow depth and bulk (snow plus ice) integrated extinction coefficients; indicating a strong influence of snow cover in controlling bottom ice algal biomass. The maximum bottom MYI chl a concentration was observed in a hummock, representing the thickest ice with lowest snow depth of this study. Hence, in this and other studies MYI chl a biomass may be under-estimated due to an under-representation of thick MYI (e.g., hummocks), which typically have a relatively thin snowpack allowing for increased light transmission. Therefore, we suggest the on-going loss of MYI in the Arctic Ocean may have a larger impact on ice–associated production than generally assumed. 相似文献
172.
Kazuhiko Yanai Naoto Yagi Takehiko Watanabe Masatosi Itoh Kiichi Ishiwata Tatsuo Ido Taiju Matsuzawa 《Journal of neurochemistry》1990,55(2):409-420
The binding of [3H]pyrilamine, a selective ligand of histamine H1 receptors, to guinea pig brain in vivo was compared with its binding to a brain homogenate. The pharmacological properties (regional distribution, saturability, and stereoselectivity) of the [3H]pyrilamine binding in vivo were similar to those of the in vitro binding to brain homogenate. A dynamic four-compartment model was proposed for the analysis of the kinetics of [3H]pyrilamine binding in vivo. The receptor constants in vivo were determined by a computer-fitting method after correcting the radioactivity of arterial plasma and brain for the presence of radioactive metabolites. The in vivo association and dissociation were 213 and 42 times, respectively, slower than those of in vitro binding at 37 degrees C. A possible mechanism for slow association and dissociation in vivo is discussed. 相似文献
173.
Tirupathi Malavath Ido Caspy Sigal Y. Netzer-El Daniel Klaiman Nathan Nelson 《BBA》2018,1859(9):645-654
The ability of photosynthetic organisms to use the sun's light as a sole source of energy sustains life on our planet. Photosystems I (PSI) and II (PSII) are large, multi-subunit, pigment–protein complexes that enable photosynthesis, but this intriguing process remains to be explained fully. Currently, crystal structures of these complexes are available for thermophilic prokaryotic cyanobacteria. The mega-Dalton trimeric PSI complex from thermophilic cyanobacterium, Thermosynechococcus elongatus, was solved at 2.5?Å resolution with X-ray crystallography. That structure revealed the positions of 12 protein subunits (PsaA-F, PsaI-M, and PsaX) and 127 cofactors.Although mesophilic organisms perform most of the world's photosynthesis, no well-resolved trimeric structure of a mesophilic organism exists. Our research model for a mesophilic cyanobacterium was Synechocystis sp. PCC6803. This study aimed to obtain well-resolved crystal structures of [1] a monomeric PSI with all subunits, [2] a trimeric PSI with a reduced number of subunits, and [3] the full, trimeric wild-type PSI complex. We only partially succeeded with the first two structures, but we successfully produced the trimeric PSI structure at 2.5?Å resolution. This structure was comparable to that of the thermophilic species, but we provided more detail. The PSI trimeric supercomplex consisted of 33 protein subunits, 72 carotenoids, 285 chlorophyll a molecules, 51 lipids, 9 iron-sulfur clusters, 6 plastoquinones, 6 putative calcium ions, and over 870 water molecules.This study showed that the structure of the PSI in Synechocystis sp. PCC6803 differed from previously described PSI structures. These findings have broadened our understanding of PSI structure. 相似文献
174.
The presence of kleptoparasitic fledglings is associated with a reduced breeding success in the host family in the barn owl
下载免费PDF全文
![点击此处可从《Journal of avian biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Fledgling birds sometimes abandon their own nest and move to neighboring nests where they are fed by host parents. This behaviour, referred to as ‘nest‐switching’, is well known in precocial birds that are mobile soon after hatching and can easily reach foster nests. In contrast, due to the difficulty of observing nest‐switching in territorial altricial birds, the causes and consequences of moving to others’ nests are poorly known in this group of birds. Nest‐switchers can be adopted by the foster parents or they can steal food from the host parents meant for their offspring, a form of kleptoparasitism, which may result in reduced breeding success of the host nest. In Israel, 12 barn owl fledglings left their natal nests and were found in 9 host nests out of 111 monitored nests (8.1%). Nest‐switchers that fledged earlier in the breeding season flew shorter distances to reach host nests probably because the density of nests with younger nestlings is higher early in the season. The number of host nestlings fledged and the percentage of nestlings fledged was lower in host nests than in nests without switchers. The occasional nest‐switchers were always older than host nestlings (respectively 80 and 50 days of age, on average) and host parents fledged fewer young when nest‐switchers occupied host nests with younger nestlings. This suggests that nest‐switchers are kleptoparasites because the presence of the older alien fledglings is associated with a lower breeding success of the host parents. 相似文献
175.
René Uebe Noa Keren‐Khadmy Natalie Zeytuni Emanuel Katzmann Yotam Navon Geula Davidov Ronit Bitton Jürgen M. Plitzko Dirk Schüler Raz Zarivach 《Molecular microbiology》2018,107(4):542-557
Magnetospirillum gryphiswaldense MSR‐1 synthesizes membrane‐enclosed magnetite (Fe3O4) nanoparticles, magnetosomes, for magnetotaxis. Formation of these organelles involves a complex process comprising key steps which are governed by specific magnetosome‐associated proteins. MamB, a cation diffusion facilitator (CDF) family member has been implicated in magnetosome‐directed iron transport. However, deletion mutagenesis studies revealed that MamB is essential for the formation of magnetosome membrane vesicles, but its precise role remains elusive. In this study, we employed a multi‐disciplinary approach to define the role of MamB during magnetosome formation. Using site‐directed mutagenesis complemented by structural analyses, fluorescence microscopy and cryo‐electron tomography, we show that MamB is most likely an active magnetosome‐directed transporter serving two distinct, yet essential functions. First, MamB initiates magnetosome vesicle formation in a transport‐independent process, probably by serving as a landmark protein. Second, MamB transport activity is required for magnetite nucleation. Furthermore, by determining the crystal structure of the MamB cytosolic C‐terminal domain, we also provide mechanistic insight into transport regulation. Additionally, we present evidence that magnetosome vesicle growth and chain formation are independent of magnetite nucleation and magnetic interactions respectively. Together, our data provide novel insight into the role of the key bifunctional magnetosome protein MamB, and the early steps of magnetosome formation. 相似文献
176.
N. Kondo Manabu Nakadome Keren Zhang Tomoyuki Shiojiri Manabu Shibasaki Kozo Hirata Atsushi Iwata 《International journal of biometeorology》1997,40(2):99-102
The purpose of this study was to investigate whether there are any effects of skin temperature changes on sweating response
in the first few minutes of mild exercise. Six healthy males performed a bicycle exercise at 100 W (50 rpm) for 30 min under
an ambient temperature of 23° C (40% RH). Esophageal temperature (T
es), mean skin temperature (T–
sk), local skin temperature at the lower left scapula (T
sl), local sweating rate (M.
sw), and cutaneous blood flow by laser-Doppler flowmetry (LDF) were measured continuously. Although T
sl decreased markedly just after the onset of sweating, T–
sk did not change. M.
sw did not increase constantly in the early stages of exercise, and there was a temporary interruption in the increase of M.
sw. This interruption in sweating was affected by the rate of change in T
sl rather than by the absolute value of T
sl, since there was a positive and significant correlation between the time of the interruption in the increase of M.
sw and the rate of decrease in T
sl (y=6.47x+0.04; r=0.86, P<0.05). The results suggest that sweating response in the early stages of exercise may be influenced by changes in local skin
temperature due to evaporative cooling.
Received: 31 August 1995 / Revised: 26 February 1996 / Accepted: 26 July 1996, 相似文献
177.
Ido Vlaardingerbroek Bas Beerens Sarah M. Schmidt Ben J. C. Cornelissen Martijn Rep 《Molecular Plant Pathology》2016,17(9):1455-1466
The genomes of many filamentous fungi consist of a ‘core’ part containing conserved genes essential for normal development as well as conditionally dispensable (CD) or lineage‐specific (LS) chromosomes. In the plant‐pathogenic fungus Fusarium oxysporum f. sp. lycopersici, one LS chromosome harbours effector genes that contribute to pathogenicity. We employed flow cytometry to select for events of spontaneous (partial) loss of either the two smallest LS chromosomes or two different core chromosomes. We determined the rate of spontaneous loss of the ‘effector’ LS chromosome in vitro at around 1 in 35 000 spores. In addition, a viable strain was obtained lacking chromosome 12, which is considered to be a part of the core genome. We also isolated strains carrying approximately 1‐Mb deletions in the LS chromosomes and in the dispensable core chromosome. The large core chromosome 1 was never observed to sustain deletions over 200 kb. Whole‐genome sequencing revealed that some of the sites at which the deletions occurred were the same in several independent strains obtained for the two chromosomes tested, indicating the existence of deletion hotspots. For the core chromosome, this deletion hotspot was the site of insertion of the marker used to select for loss events. Loss of the core chromosome did not affect pathogenicity, whereas loss of the effector chromosome led to a complete loss of pathogenicity. 相似文献
178.
M.-T. Rofe Y. Shacham A. Steinvi L. Barak M. Hareuveni S. Banai G. Keren A. Finkelstein H. Shmilovich 《Netherlands heart journal》2016,24(5):350-354
Objectives
To assess the prevalence of blood type A among patients referred for transcatheter aortic valve implantation (TAVI) and whether it is related to vascular complications.Backgrounds
Vascular complications following TAVI are associated with adverse outcomes. Various blood types, particularly type A, have been shown to be more prevalent in cardiovascular diseases and to be related to prognosis.Methods
The prevalence of various blood types in a cohort of 491 consecutive patients who underwent TAVI was compared with a control group of 6500 consecutive hospitalised patients. The prevalence and predictors of vascular complications and bleeding events were evaluated in the blood type A group and were compared with non-type A patients.Results
The mean age of TAVI patients was 83?±?6 years, and 40?% were males. Patients were divided into two groups: blood type A (n?=?220) and non-type A (n?=?271). Type A was significantly more prevalent in the TAVI group than in the control group (45 vs. 38?%, p?=?0.023). Compared with the non-type A group, patients with blood type A had more major and fatal bleeding (14.5 vs. 8.1?%, p?=?0.027) and more vascular complications (any vascular complication: 24.5 vs. 15.9?% p?=?0.016; major vascular complications: 12.3 vs. 7?% p?=?0.047). In a multivariable analysis, blood type A emerged as a significant and independent predictor for vascular complications and bleeding events.Conclusions
Blood type A is significantly more prevalent in TAVI patients than in the general population and is related to higher rates of vascular and bleeding complications.179.
Adult passerines renew their flight feathers at least once every year. This complete moult occurs either in the breeding areas, just after breeding (summer moult), or, in some long-distance migratory species, at the non-breeding areas, after arrival to the southern wintering area at the end of autumn migration (winter moult). The aim of this study was to relate moult strategies with the DMD, the difference in median migration date, through Israel, between juveniles and adults. Our data on autumn migration timing in juveniles and adults was based on ringing data of 49,125 individuals belonging to 23 passerine species that breed in Europe and Western Asia and migrate through Israel. We found that DMD was associated with moult timing. In all species that perform a winter moult, adults preceded juveniles during autumn. Among migrants who perform a summer moult, we found evidence of both migration timing patterns: juveniles preceding adults or adults preceding juveniles. In addition, in summer moulters, we found a significant, positive correlation between mean breeding latitude and DMD. Although previous studies described that moult duration and extent can be affected by migration, we suggest that moult strategies affect both migration timing and migration strategy. These two moult strategies (summer or winter moult) also represent two unique migration strategies. Our findings highlight the evolutionary interplay between moult and migration strategies. 相似文献
180.
Effects of salinity and varying boron concentrations on boron uptake and growth of wheat 总被引:1,自引:0,他引:1
Summary Two sandculture experiments were conducted with wheat (Triticum aestivum) to determine the effects of (1) osmotic potential (Ψπ) and (2) fluctuating boron (B) concentrations on B availability (toxicity), shoot growth and leaf concentrations of B of
wheat. The first experiment consisted of growing wheat to the spike emergence stage in sandcultures irrigated with a complete
nutrient solution containing 1.0, 7.5, and 15.0 mg Bl−1 and having Ψπ values of −0.02, −0.07, −0.12, and −0.17 MPa produced by CaCl2−NaCl additions. Statistically, shoot weight was independently influenced by the B and Ψπ treatments but not by their interaction. Only the B treatment had a significant effect on leaf boron concentrations; the
B x Ψπ interaction was nonsignificant with respect to leaf B concentrations.
The second experiment was designed to determine if growth and B uptake of wheat responds to the time integrated mean (TIM)
concentration of B. This experiment consisted of four fixed-B concentrations and four fluctuating-B concentrations designed
to produce two TIM concentrations (3.9 and 7.4 mg Bl−1) approached low to high and vice versa. With respect to shoot weight, there was no statistical difference among treatments
having the same TIM concentration during the 10 week experiment. However, shoot B concentrations differed greatly; they were
higher when the B concentration was progressively increased over the 10 week period. Leaf B concentrations (Y leaf at flowering),
while not as high as the shoot B concentrations, were also higher under the treatment of increasing B concentration, indicating
B uptake rates are higher for mature plants than for seedlings. 相似文献