全文获取类型
收费全文 | 15526篇 |
免费 | 1368篇 |
国内免费 | 13篇 |
出版年
2022年 | 96篇 |
2021年 | 196篇 |
2020年 | 127篇 |
2019年 | 164篇 |
2018年 | 229篇 |
2017年 | 170篇 |
2016年 | 329篇 |
2015年 | 545篇 |
2014年 | 628篇 |
2013年 | 764篇 |
2012年 | 1022篇 |
2011年 | 976篇 |
2010年 | 644篇 |
2009年 | 591篇 |
2008年 | 897篇 |
2007年 | 833篇 |
2006年 | 788篇 |
2005年 | 828篇 |
2004年 | 804篇 |
2003年 | 777篇 |
2002年 | 776篇 |
2001年 | 214篇 |
2000年 | 149篇 |
1999年 | 236篇 |
1998年 | 223篇 |
1997年 | 149篇 |
1996年 | 152篇 |
1995年 | 146篇 |
1994年 | 144篇 |
1993年 | 130篇 |
1992年 | 150篇 |
1991年 | 118篇 |
1990年 | 133篇 |
1989年 | 116篇 |
1988年 | 124篇 |
1987年 | 120篇 |
1986年 | 116篇 |
1985年 | 137篇 |
1984年 | 155篇 |
1983年 | 127篇 |
1982年 | 146篇 |
1981年 | 154篇 |
1980年 | 158篇 |
1979年 | 106篇 |
1978年 | 105篇 |
1977年 | 95篇 |
1976年 | 82篇 |
1975年 | 85篇 |
1974年 | 110篇 |
1973年 | 89篇 |
排序方式: 共有10000条查询结果,搜索用时 78 毫秒
21.
22.
23.
24.
Bioaccessibility measurements have the potential to improve the accuracy of risk assessments and reduce the potential costs of remediation when they reveal that the solubility of chemicals in a matrix (e.g., soil) differs markedly from that in the critical toxicity study (i.e., the key study from which a toxicological or toxicity reference value is derived). We aimed to apply this approach to a brownfield site contaminated with chromium, and found that the speciation was CrIII, using a combination of alkaline digestion/diphenylcarbazide complexation and X-ray absorption near edge structure analysis. The bioaccessibility of Cr2O3, the compound on which a reference dose for CrIII is based, was substantially lower (<0.1%) than that of the CrIII in the soils, which was a maximum of 9%, giving relative bioaccessibility values of 13,000% in soil. This shows that the reference dose is based on essentially an insoluble compound, and thus we suggest that other compounds be considered for toxicity testing and derivation of reference dose. Two possibilities are CrCl3·6H2O and KCr(SO4)2·12H2O, which have been used for derivation of ecological toxicity reference values and are soluble at a range of dosing levels in our bioaccessibility tests. 相似文献
25.
26.
Protease levels in human blood are often prognostic indicators of inflammatory, thrombotic or oncogenic disorders. The measurement of such enzyme activities in substrate-based assays is complicated due to the low prevalence of these enzymes and steric hindrance of the substrates by the more abundant blood proteins. To address these limitations, we developed a molecular construct that is suitable for microsphere-cytometer based assays in the milieu of human blood plasma. In this proof of principle study, we demonstrate the utility of this substrate to measure metalloprotease ADAMTS13 activity. The substrate, expressed in E. coli as a fusion protein, contains the partial A2-domain of von Willebrand factor (VWF amino acids 1594–1670) that is mutated to include a single primary amine at the N-terminus and free cysteines at the C-terminus. N-terminus fluorescence conjugation was possible using NHS (N-hydroxysuccinimide) chemistry. Maleimide-PEG(Polyethylene glycol)n-biotin coupling at the C-terminus allowed biotinylation with variable PEG spacer lengths. Once bound to streptavidin-bearing microspheres, the substrate fluorescence signal decreased in proportion with ADAMTS13 concentration. Whereas recombinant ADAMTS13 activity could be quantified using substrates with all PEG repeat-lengths, only the construct with the longer 77 PEG-unit could quantify proteolysis in blood plasma. Using this longer substrate, plasma ADAMTS13 down to 5% of normal levels could be detected within 30 min. Such measurements could also be readily performed under conditions resembling hyperbilirubinemia. Enzyme catalytic activity was tuned by varying buffer calcium, with lower divalent ion concentrations enhancing cleavage. Overall, the study highlights the substrate design features important for the creation of efficient proteolysis assays in the setting of human plasma. In particular, it emphasizes the need to introduce PEG spacers in plasma-based experiments, a design attribute commonly ignored in immobilized peptide-substrate assays. 相似文献
27.
28.
Controlled generation of reactive oxygen species orchestrates numerous physiological signaling events (Finkel, T. (2011) Signal transduction by reactive oxygen species. J. Cell Biol. 194, 7–15). A major cellular target of reactive oxygen species is the thiol side chain (RSH) of Cys, which may assume a wide range of oxidation states (i.e. −2 to +4). Within this context, Cys sulfenic (Cys-SOH) and sulfinic (Cys-SO2H) acids have emerged as important mechanisms for regulation of protein function. Although this area has been under investigation for over a decade, the scope and biological role of sulfenic/sulfinic acid modifications have been recently expanded with the introduction of new tools for monitoring cysteine oxidation in vitro and directly in cells. This minireview discusses selected recent examples of protein sulfenylation and sulfinylation from the literature, highlighting the role of these post-translational modifications in cell signaling. 相似文献
29.
30.