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51.
The genotoxicity and mutagenicity of several kinds of quinone pigments from pathogenic fungi were examined by means of the hepatocyte primary culture (HPC)/DNA repair test and of Ames test with TA98 and TA100. Clear genotoxicity of the two quinone chemicals, xanthomegnin and luteosporin were observed in the HPC/DNA repair test, though definite mutagenicity was not detected in the Salmonella microsome test. These two pigments are thus suspected to be genotoxic carcinogens. 相似文献
52.
The effect of an analog of LH-RH, (Des-Gly-NH210, Proethylamide9)-LH-RH, on LH release, was studied in one bull with azooapermia, and one cow with cystic follicle. The single intravenous injection of 100 μg LH-RH analog caused abrupt and marked increase in serum LH and the high levels of LH were maintained for 3 hours. The highest levels of serum LH were 48 and 114 times as high as the pre-treatment levels in a bull and a cow, respectively. Further studies are being undertaken to evaluate this compound for improving reproductive function in cattle. 相似文献
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Tetsuya Tosa Tadashi Sato Takao Mori Yuhsi Matuo Ichiro Chibata 《Biotechnology and bioengineering》1973,15(1):69-84
Various methods were tried for the immobilization of aspartase, and the preparation having the highest activity was obtained when partially purified aspartase from Escherichia coli was entrapped into polyacrylamide gel Iattice. Enzymatic properties of the immobilized aspartase were investigated and compared with those of the native aspartase. With regard to optimum pH, temperature, concentration of Mn++, kinetic constants and heat stability, no marked difference was observed between the native and immobilized aspartases. By employing an enzyme column packed with the immobilized aspartase, conditions for continuous production of L -aspartic acid from ammonium fumarate were investigated. When a solution of 1M ammonium fumarate (pH 8.5, containing 1mM MnCl2) was passed through the aspartase column at the flow rate of SV = 0.08 at 37°C, the highest rate of reaction was attained. From the column effluents, L-aspartic acid was obtained in a good yield. 相似文献
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Summary Histochemical study in epiphyseal cartilages and periodontal tissues of the rat subjected to cortisone and PTH administration was examined enzyme histochemically in the decalcified frozen sections with neutral EDTA.In cortisone and PTH-treated rats, altered proliferating cartilage cells in the epiphysis revealed a weak, and decreasing activities of oxidative enzymes with the comparison to those of the control, however, they showed similar activity for alkaline and acid phosphatases as normal cells.Epiphysis and alveolar bones in large dose of cortisone administered rats exhibited an absence or markedly decreasing number of osteoclasts, but on the contrary, those of PTH treated rats led to an increased number of the cells. Osteoclasts in hormone treated conditions were characterized by the existence of the strong succinate dehydrogenase activity as well as in normal status. 相似文献
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Summary Zymogramic analysis of esterase in the mouse, rat and guinea-pig salivary glands was undertaken and demonstrated species and organ specificities of esterase with electrophoretic method. Salivary glands esterase was classified into A, B and C types based on the electrophoretic mobility. Mouse submandibular gland had the most complicated pattern, while guinea-pig showed the simpliest patterns which was devoid of B type of esterase. Rat salivary glands exhibited rather regular patterns. Similar zymogram patterns were obtained with many kinds of ester compounds, that is simple and substituted naphthol esters and indoxyl derivatives. The tests of inhibition and activation for esterase activity was obtained. Histochemical properties applied to inhibitor test in the esterase zymogram patterns showed no marked differences between ducts and acini. 相似文献
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