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991.
Tatsuru Kadota Jun Osato Ken Nagata Yoichi Sakai 《Ethology : formerly Zeitschrift fur Tierpsychologie》2012,118(3):226-234
Bi‐directional sex change has recently been reported in a range of reef fishes, including haremic species that were earlier thought to be protogynous (female to male). However, the occurrence of this phenomenon and the social conditions driving the reversion of males to females (reversed sex change) have been poorly documented under natural conditions. Reversed sex change is predicted to occur in low‐density populations where facultative monogamy is common. However, few studies have evaluated this over a long period in such populations. We documented the occurrence of bi‐directional sex change during a 3‐yr demographic survey of a population characterised by small harem sizes in haremic hawkfish Cirrhitichthys falco. New males were derived following a change in sex of functional females (secondary males; n = 3) and juveniles always matured first as females (n = 3). Thus, C. falco exhibited a typical protogynous sexual pattern, consistent with a range of haremic fish species. We observed reversed sex change in two males. In both cases, all the females disappeared from their harems and the neighbouring males expanded their territories to encompass the territories of the sex changers. However, bachelor males did not always revert to females. A dominant male experienced bachelor status twice but regained mating opportunities following the immigration of a female into his territory or by taking a female from a neighbouring harem. Thus, we conclude that bachelor males use reversed sex change as a facultative tactic to regain reproductive status in a haremic mating system. In addition, we discuss the influence of harem size upon occurrence of reversed sex change. 相似文献
992.
Kanie T Onoyama I Matsumoto A Yamada M Nakatsumi H Tateishi Y Yamamura S Tsunematsu R Matsumoto M Nakayama KI 《Molecular and cellular biology》2012,32(3):590-605
D-type cyclins play a pivotal role in G(1)-S progression of the cell cycle, and their expression is frequently deregulated in cancer. Cyclin D1 has a half-life of only ~30 min as a result of its ubiquitylation and proteasomal degradation, with various F-box proteins, including Fbxo4, Fbxw8, Skp2, and Fbxo31, having been found to contribute to its ubiquitylation. We have now generated Fbxo4-deficient mice and found no abnormalities in these animals. Cyclin D1 accumulation was thus not observed in Fbxo4(-/-) mouse tissues. The half-life of cyclin D1 in mouse embryonic fibroblasts (MEFs) prepared from Fbxo4(-/-), Fbxw8(-/-), and Fbxo4(-/-); Fbxw8(-/-) mice also did not differ from that in wild-type MEFs. Additional depletion of Skp2 and Fbxo31 in Fbxo4(-/-); Fbxw8(-/-) MEFs by RNA interference did not affect cyclin D1 stability. Although Fbxo31 depletion in MEFs increased cyclin D1 abundance, this effect appeared attributable to upregulation of cyclin D1 mRNA. Furthermore, abrogation of the function of the Skp1-Cul1-F-box protein (SCF) complex or the anaphase-promoting complex/cyclosome (APC/C) complexes did not alter the half-life of cyclin D1, whereas cyclin D1 degradation was dependent largely on proteasome activity. Our genetic analyses thus do not support a role for any of the four F-box proteins examined in cyclin D1 degradation during normal cell cycle progression. They suggest the existence of other ubiquitin ligases that target cyclin D1 for proteolysis. 相似文献
993.
994.
Masashi Kondo Ken Maeda Nozomi Yamasaki Katsunori Tachihara 《Environmental Biology of Fishes》2012,95(2):291-300
Egg masses of Luciogobius ryukyuensis were found in spawning grounds around the lowest reach of the adult??s habitat in the tidally influenced area of streams on Okinawa Island. The eggs were attached to the underside of stones and were cared for by a solitary male fish. The number of eggs within an egg mass was 191?C1368. The eggs were elliptical, measuring 2.0?C2.6?mm in length, and 0.6?C0.8?mm in width. Early development of L. ryukyuensis was described from laboratory-reared specimens. Newly hatched larvae, measuring 2.8?C3.3?mm in body length, had an open mouth, pigmented eyes, pectoral fin buds, an orange-colored yolk sac, and characteristic melanophores along the dorsal and ventral midlines of the body. The yolk was completely absorbed at days 2?C3. Notochord flexion began at day 10 and was completed at day 15. The fish started settling on the bottom of the tank at day 34 (14.1?mm in body length) when the body surface started to be covered by intense pigmentation. The eggs and newly hatched larvae of L. ryukyuensis were of standard size of the genus and their morphologies closely resemble those of L. guttatus. 相似文献
995.
H Inuzuka D Gao LW Finley W Yang L Wan H Fukushima YR Chin B Zhai S Shaik AW Lau Z Wang SP Gygi K Nakayama J Teruya-Feldstein A Toker MC Haigis PP Pandolfi W Wei 《Cell》2012,150(1):179-193
Aberrant Skp2 signaling has been implicated as a driving event in tumorigenesis. Although the underlying molecular mechanisms remain elusive, cytoplasmic Skp2 correlates with more aggressive forms of breast and prostate cancers. Here, we report that Skp2 is acetylated by p300 at K68 and K71, which is a process that can be antagonized by the SIRT3 deacetylase. Inactivation of SIRT3 leads to elevated Skp2 acetylation, which leads to increased Skp2 stability through impairment of the Cdh1-mediated proteolysis pathway. As a result, Skp2 oncogenic function is increased, whereby cells expressing an acetylation-mimetic mutant display enhanced cellular proliferation and tumorigenesis in vivo. Moreover, acetylation of Skp2 in the nuclear localization signal (NLS) promotes its cytoplasmic retention, and cytoplasmic Skp2 enhances cellular migration through ubiquitination and destruction of E-cadherin. Thus, our study identifies an acetylation-dependent regulatory mechanism governing Skp2 oncogenic function and provides insight into how cytoplasmic Skp2 controls cellular migration. 相似文献
996.
Ramaswamy Govindan Li Ding Malachi Griffith Janakiraman Subramanian Nathan D. DeesKrishna L. Kanchi Christopher A. Maher Robert Fulton Lucinda Fulton John Wallis Ken ChenJason Walker Sandra McDonald Ron Bose David OrnitzDonghai Xiong Ming YouDavid J. Dooling Mark Watson Elaine R. Mardis Richard K. Wilson 《Cell》2012,150(6):1121-1134
997.
Yoshida-Takashima Y Nunoura T Kazama H Noguchi T Inoue K Akashi H Yamanaka T Toki T Yamamoto M Furushima Y Ueno Y Yamamoto H Takai K 《Applied and environmental microbiology》2012,78(5):1311-1320
Viruses play important roles in marine surface ecosystems, but little is known about viral ecology and virus-mediated processes in deep-sea hydrothermal microbial communities. In this study, we examined virus-like particle (VLP) abundances in planktonic and attached microbial communities, which occur in physical and chemical gradients in both deep and shallow submarine hydrothermal environments (mixing waters between hydrothermal fluids and ambient seawater and dense microbial communities attached to chimney surface areas or macrofaunal bodies and colonies). We found that viruses were widely distributed in a variety of hydrothermal microbial habitats, with the exception of the interior parts of hydrothermal chimney structures. The VLP abundance and VLP-to-prokaryote ratio (VPR) in the planktonic habitats increased as the ratio of hydrothermal fluid to mixing water increased. On the other hand, the VLP abundance in attached microbial communities was significantly and positively correlated with the whole prokaryotic abundance; however, the VPRs were always much lower than those for the surrounding hydrothermal waters. This is the first report to show VLP abundance in the attached microbial communities of submarine hydrothermal environments, which presented VPR values significantly lower than those in planktonic microbial communities reported before. These results suggested that viral lifestyles (e.g., lysogenic prevalence) and virus interactions with prokaryotes are significantly different among the planktonic and attached microbial communities that are developing in the submarine hydrothermal environments. 相似文献
998.
999.
Ozaki S Imai H Iwakiri T Sato T Shimoda K Nakayama T Hamada H 《Biotechnology letters》2012,34(3):475-481
A glucosyltransferase (GT) of Phytolacca americana (PaGT3) was expressed in Escherichia coli and purified for the synthesis of two O-β-glucoside products of trans-resveratrol. The reaction was moderately regioselective with a ratio of 4′-O-β-glucoside: 3-O-β-glucoside at 10:3. We used not only the purified enzyme but also the E. coli cells containing the PaGT3 gene for the synthesis of glycoconjugates. E. coli cell cultures also have other advantages, such as a shorter incubation time compared with cultured plant cells, no need for
the addition of exogenous glucosyl donor compounds such as UDP-glucose, and almost complete conversion of the aglycone to
the glucoside products. Furthermore, a homology model of PaGT3 and mutagenesis studies suggested that His-20 would be a catalytically
important residue. 相似文献
1000.
Effect of temperature on gelatinization and retrogradation in high hydrostatic pressure treatment of potato starch-water mixtures 总被引:3,自引:0,他引:3
The effect of temperature (20-70 °C) on the gelatinization and retrogradation of potato starch-water mixtures (10-70%, w/w) treated with high hydrostatic pressure (HHP) (400-1000 MPa) was investigated. Gelatinization enthalpy change (ΔHgel) and re-gelatinization enthalpy change of retrograded crystalline part (ΔHretro) of the HHP-treated starch were evaluated using differential scanning calorimetry. The value of ΔHgel of 10-20% (w/w) mixtures decreased with increased pressure and temperature, while ΔHgel of 30-50% (w/w) mixtures decreased to certain values with increased pressure and the values depended on treatment temperature. With higher temperature and pressure conditions, ΔHgel of 10-40% (w/w) mixtures reached zero, but ΔHgel of 50-70% (w/w) mixtures did not. Retrogradation was observed with HHP-treated 20-60% (w/w) mixtures and the value of ΔHretro depended on the starch content, pressure, and temperature. The value of ΔHretro trended to increase with increase in starch content. In addition, retrogradation was promoted by HHP treatment at low temperature. Gelatinizaiton and retrogradation behaviors of HHP-treated (400-1000 MPa) potato starch-water mixtures (10-70%, w/w) at 20-70 °C were summerized in a series of state diagrams. 相似文献