MYH9: structure,functions and role of non-muscle myosin ⅡA in human disease

MYH9-related diseases (MYH9-RD) are a group of autosomal dominant diseases caused by mutations in the MYH9 gene, which are featured by thrombocytopenia, giant platelets and granulocyte cytoplasmic inclusion bodies. MYH9-RD patients generally suffer from bleeding syndromes, progressive kidney disease, deafness, or cataracts. Here, we reported on a case of MYH9-RD. A novel heterozygous mutation of MYH9 (c.2344-2345delGTinsTA, p.T782Y) was discovered by targeted sequencing technology. Immunofluorescence analysis of neutrophils confirmed abnormal aggregation of MYH9 protein. The results of this study should expand the MYH9 gene mutation spectrum and provide reference for subsequent researchers and genetic counseling.     

DNA framework-engineered electrochemical biosensors

Self-assembled DNA nanostructures have shown remarkable potential in the engineering of biosensing interfaces, which can improve the performance of various biosensors. In particular, by exploiting the structural rigidity and programmability of the framework nucleic acids with high precision, molecular recognition on the electrochemical biosensing interface has been significantly enhanced, leading to the development of highly sensitive and specific biosensors for nucleic acids, small molecules,proteins, and cells. In this review, we summarize recent advances in DNA framework-engineered biosensing interfaces and the application of corresponding electrochemical biosensors.     

Effects of Temperature on Transcriptome Profiles in Cardiocrinum cathayanum Leaves

Russian Journal of Plant Physiology - Cardiocrinum cathayanum (Endl.) Lindl. (Liliaceae) is a promising species for ornamental and pharmaceutical usage. However, genomic responses of C. cathayanum...     

Eukaryotic translation initiation factor 3 subunit b is a novel oncogenic factor in prostate cancer

Mammalian Genome - Prostate cancer, the second most common cancer among male adults, affects millions globally. We sought to investigate the expression and contribution of Eukaryotic translation...     

Structural and biochemical characterization of SADS‐CoV papain‐like protease 2

Swine acute diarrhea syndrome coronavirus (SADS‐CoV) is a novel coronavirus that is involved in severe diarrhea disease in piglets, causing considerable agricultural and economic loss in China. The emergence of this new coronavirus increases the importance of understanding SADS‐CoV as well as antivirals. Coronaviral proteases, including main proteases and papain‐like proteases (PLP), are attractive antiviral targets because of their essential roles in polyprotein processing and thus viral maturation. Here, we describe the biochemical and structural identification of recombinant SADS papain‐like protease 2 (PLP2) domain of nsp3. The SADS‐CoV PLP2 was shown to cleave nsp1 proteins and also peptides mimicking the nsp2|nsp3 cleavage site and also had deubiquitinating and deISGynating activity by in vitro assays. The crystal structure adopts an architecture resembling that of PLPs from other coronaviruses. We characterize both conserved and unique structural features likely directing the interaction of PLP2 with the substrates, including the tentative mapping of active site and other essential residues. These results provide a foundation for understanding the molecular basis of coronaviral PLPs' catalytic mechanism and for the screening and design of therapeutics to combat infection by SADS coronavirus.     

退化高寒草甸狼毒发生区土壤真菌多样性的空间变异

狼毒是青藏高原危害最严重的毒草种类之一,其快速蔓延对高寒草甸生态系统的影响日益严重。选取祁连山中段退化高寒草甸为研究区,综合采用高通量测序技术、地统计学和GIS空间分析方法,分析狼毒发生区土壤真菌多样性的空间变异特征,研究狼毒群落与土壤真菌多样性的空间相关性。结果表明: 与非发生区相比,狼毒发生区土壤真菌群落物种丰富度下降而优势度显著增加,α多样性降低;土壤真菌群落物种构成差异增强,β多样性明显升高。狼毒入侵对土壤真菌多样性的空间格局有一定扰动,发生区各多样性指数的斑块破碎化程度增加,土壤真菌群落物种构成的空间异质性明显增强,α和β多样性的空间稳定性降低。狼毒盖度与土壤真菌α和β多样性指数呈现显著正相关及显著负相关的区域交错镶嵌分布,空间相关性规律不明显,表明狼毒入侵草甸土壤真菌多样性的空间变异可能受地上植被和土壤环境的共同作用。     

Meeting report on the 2019 international symposium of molecular design breeding in animals (Yangling,China) with the consensus on genome-editing agricultural animals and their regulation

Transgenic Research -