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101.
Indomethacin inhibition of ovulation in the cow 总被引:1,自引:0,他引:1
Indomethacin or saline was administered via intramuscular, intrauterine or intraovarian routes to dairy cows, within 24 h after standing oestrus was first observed. The incidence of ovulation was determined at slaughter. All of the saline-treated cows (18/18) ovulated. Ovulation was not blocked after intramuscular injection (0/6) or intrauterine infusion (0/6) of indomethacin. In all cows, ovulation was blocked after intraovarian injection (6/6) of indomethacin. These findings add support to the hypothesis that prostaglandins play an essential role in ovulation in the cow as in many other mammalian species. 相似文献
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105.
Biophysics - The effects of the separate and combined action of lead nitrate in a wide range of doses, uranyl nitrate, and chronic low-dose gamma irradiation on the formation of oxidative stress in... 相似文献
106.
Zubareva O. E. Melik-Kasumov T. B. 《Journal of Evolutionary Biochemistry and Physiology》2021,57(4):743-760
Journal of Evolutionary Biochemistry and Physiology - A large proportion of patients with epilepsy suffer from pharmacoresistant forms of the disease, and this makes the search for new treatments... 相似文献
107.
Blazhevich L. E. Smirnova O. E. Kirilina V. M. Krivchenko A. I. 《Journal of Evolutionary Biochemistry and Physiology》2021,57(4):953-961
Journal of Evolutionary Biochemistry and Physiology - The article addresses the role of TNF-α, IL-2, IL-5 and IL-6 in the contractile activity of rat tracheal and bronchial smooth muscle... 相似文献
108.
When nutrients are depleted, Dictyostelium cells undergo cell cycle arrest and initiate a developmental program that ensures survival. The YakA protein kinase governs this transition by regulating the cell cycle, repressing growth-phase genes and inducing developmental genes. YakA mutants have a shortened cell cycle and do not initiate development. A suppressor of yakA that reverses most of the developmental defects of yakA- cells, but none of their growth defects was identified. The inactivated gene, pufA, encodes a member of the Puf protein family of translational regulators. Upon starvation, pufA- cells develop precociously and overexpress developmentally important proteins, including the catalytic subunit of cAMP-dependent protein kinase, PKA-C. Gel mobility-shift assays using a 200-base segment of PKA-C's mRNA as a probe reveals a complex with wild-type cell extracts, but not with pufA- cell extracts, suggesting the presence of a potential PufA recognition element in the PKA-C mRNA. PKA-C protein levels are low at the times of development when this complex is detectable, whereas when the complex is undetectable PKA-C levels are high. There is also an inverse relationship between PufA and PKA-C protein levels at all times of development in every mutant tested. Furthermore, expression of the putative PufA recognition elements in wild-type cells causes precocious aggregation and PKA-C overexpression, phenocopying a pufA mutation. Finally, YakA function is required for the decline of PufA protein and mRNA levels in the first 4 hours of development. We propose that PufA is a translational regulator that directly controls PKA-C synthesis and that YakA regulates the initiation of development by inhibiting the expression of PufA. Our work also suggests that Puf protein translational regulation evolved prior to the radiation of metazoan species. 相似文献
109.
Liposomes composed of soybean phosphatidylcholine were peroxidized using the reagent sodium hypochlorite or the myeloperoxidase-hydrogen peroxide-Cl- system. Linoleic acid hydroperoxide previously prepared from linoleic acid by means of lipoxidase was incorporated into liposomes. The yield of thiobarbituric acid reactive substances (TBARS) continuously increased with higher amounts of hydroperoxide groups after the initiation of lipid peroxidation by hypochlorous acid producing systems. The accumulation of TBARS was inhibited by scavengers of free radicals such as butylated hydroxytoluene and by the scavengers of hypochlorous acid, taurine and methionine. Lipid peroxidation was also prevented by sodium azide or chloride free medium in the myeloperoxidase-hydrogen peroxide-Cl- system. Here we show for the first time that the reaction of hypochlorous acid with a biologically relevant hydroperoxide yields free radicals able to cause further oxidation of lipid molecules. 相似文献
110.
The kinetic behaviour of the ATPase activity of beef heart F1 depends largely on the exposure of the enzyme to some anionic ligands such as sulphate and/or EDTA. F1 prepared in the presence of such anions exhibited a triphasic kinetic pattern whereas F1 from which those anions were removed by dialysis exhibited only two Km values for ATP. Conversely to what has been previously reported, bicarbonate did not linearize F1-ATPase kinetics. Moreover, anion activation cannot be simply explained by promotion of ADP release but mainly by an increase in affinity of the third catalytic site for ATP. 相似文献