全文获取类型
收费全文 | 2008篇 |
免费 | 106篇 |
出版年
2023年 | 6篇 |
2022年 | 23篇 |
2021年 | 42篇 |
2020年 | 21篇 |
2019年 | 35篇 |
2018年 | 52篇 |
2017年 | 41篇 |
2016年 | 59篇 |
2015年 | 75篇 |
2014年 | 101篇 |
2013年 | 155篇 |
2012年 | 158篇 |
2011年 | 153篇 |
2010年 | 91篇 |
2009年 | 71篇 |
2008年 | 142篇 |
2007年 | 136篇 |
2006年 | 111篇 |
2005年 | 117篇 |
2004年 | 109篇 |
2003年 | 92篇 |
2002年 | 83篇 |
2001年 | 18篇 |
2000年 | 15篇 |
1999年 | 22篇 |
1998年 | 24篇 |
1997年 | 9篇 |
1996年 | 17篇 |
1995年 | 16篇 |
1994年 | 16篇 |
1993年 | 11篇 |
1992年 | 14篇 |
1991年 | 6篇 |
1990年 | 6篇 |
1989年 | 17篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1983年 | 2篇 |
1982年 | 8篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1975年 | 2篇 |
1948年 | 1篇 |
1938年 | 1篇 |
排序方式: 共有2114条查询结果,搜索用时 15 毫秒
71.
72.
73.
74.
Kazuma Okada Masato Wada Shigeki Moriya Yuichi Katayose Hiroko Fujisawa Jianzhong Wu Hiroyuki Kanamori Kanako Kurita Harumi Sasaki Hiroshi Fujii Shingo Terakami Hiroshi Iwanami Toshiya Yamamoto Kazuyuki Abe 《Journal of plant research》2016,129(6):1109-1126
Determining the molecular mechanism of fruit tree architecture is important for tree management and fruit production. An apple mutant ‘McIntosh Wijcik’, which was discovered as a bud mutation from ‘McIntosh’, exhibits a columnar growth phenotype that is controlled by a single dominant gene, Co. In this study, the mutation and the Co gene were analyzed. Fine mapping narrowed the Co region to a 101 kb region. Sequence analysis of the Co region and the original wild-type co region identified an insertion mutation of an 8202 bp long terminal repeat (LTR) retroposon in the Co region. Segregation analysis using a DNA marker based on the insertion polymorphism showed that the LTR retroposon was closely associated with the columnar growth phenotype. RNA-seq and RT-PCR analysis identified a promising Co candidate gene (91071-gene) within the Co region that is specifically expressed in ‘McIntosh Wijcik’ but not in ‘McIntosh’. The 91071-gene was located approximately 16 kb downstream of the insertion mutation and is predicted to encode a 2-oxoglutarate-dependent dioxygenase involved in an unknown reaction. Overexpression of the 91071-gene in transgenic tobaccos and apples resulted in phenotypes with short internodes, like columnar apples. These data suggested that the 8202 bp retroposon insertion in ‘McIntosh Wijcik’ is associated with the short internodes of the columnar growth phenotype via upregulated expression of the adjacent 91071-gene. Furthermore, the DNA marker based on the insertion polymorphism could be useful for the marker-assisted selection of columnar apples. 相似文献
75.
Mari Kawamura Tadashi Yamamoto Keisuke Yamashiro Shinsuke Kochi Chiaki Yoshihara‐Hirata Hidetaka Ideguchi Hiroaki Aoyagi Kazuhiro Omori Shogo Takashiba 《Journal of cellular and molecular medicine》2019,23(2):1211-1223
The recruitment of tissue‐resident stem cells is important for wound regeneration. Periodontal ligament cells (PDL cells) are heterogeneous cell populations with stemness features that migrate into wound sites to regenerate periodontal fibres and neighbouring hard tissues. Cell migration is regulated by the local microenvironment, coordinated by growth factors and the extracellular matrix (ECM). Integrin‐mediated cell adhesion to the ECM provides essential signals for migration. We hypothesized that PDL cell migration could be enhanced by selective expression of integrins. The migration of primary cultured PDL cells was induced by platelet‐derived growth factor‐BB (PDGF‐BB). The effects of blocking specific integrins on migration and ECM adhesion were investigated based on the integrin expression profiles observed during migration. Up‐regulation of integrins α3, α5, and fibronectin was identified at distinct localizations in migrating PDL cells. Treatment with anti‐integrin α5 antibodies inhibited PDL cell migration. Treatment with anti‐integrin α3, α3‐blocking peptide, and α3 siRNA significantly enhanced cell migration, comparable to treatment with PDGF‐BB. Furthermore, integrin α3 inhibition preferentially enhanced adhesion to fibronectin via integrin α5. These findings indicate that PDL cell migration is reciprocally regulated by integrin α3‐mediated inhibition and α5‐mediated promotion. Thus, targeting integrin expression is a possible therapeutic strategy for periodontal regeneration. 相似文献
76.
Keisuke Iida Yamato Tsushima Yue Ma Shadi Sedghi Masoud Mai Sakuma Tomomi Yokoyama Wataru Yoshida Kazunori Ikebukuro Kazuo Nagasawa 《Bioorganic & medicinal chemistry》2019,27(8):1742-1746
G-quadruplexes (G4s) are non-B DNA structures present in guanine-rich regions of gene regulatory areas, promoters and CpG islands, but their occurrence and functions remain incompletely understood. Thus, methodology to identify G4 sequences is needed. Here, we describe the synthesis of a novel cyclic hepta-oxazole compound, L1Bio-7OTD (1), bearing a biotin affinity-tag as a tool to pull down G4 structures from mixtures of G4-forming and non G4-forming DNA sequences. We confirmed that it could pull down G4s associated with telomeres, bcl-2 gene, and c-kit gene. 相似文献
77.
78.
79.
80.
Chan T Kondow A Hosoya A Hitachi K Yukita A Okabayashi K Nakamura H Ozawa H Kiyonari H Michiue T Ito Y Asashima M 《FEBS letters》2007,581(14):2691-2696