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21.
22.
E. Modesto P. D. Lampe M. C. Ribeiro D. C. Spray A. C. Campos de Carvalho 《The Journal of membrane biology》1996,154(3):239-249
Membrane fractions highly enriched in chicken lens MIP (MIP28) were found to form ion channels when incorporated into planar
lipid bilayers. The channels displayed prominent unitary conductances of about 60 and 290 pS in symmetric 150 mm KCl solution and were slightly anion selective. For both depolarizing and hyperpolarizing voltages, voltage sensitivity of
the MIP28-induced conductance could be fit by a Boltzmann relation, symmetric around zero mV, with V
0
= 18.5 mV, n= 4.5 and g
min/g
max= 0.17. Channel properties were not appreciably altered by pH in the range of 5.8 to 7, although channel incorporation was
observed to occur more frequently at lower pH values. Calcium, at millimolar concentrations, decreased the channel mean open
time. Partial proteolysis of MIP28 to yield MIP21 did not appreciably affect single-channel conductance or voltage sensitivity
of the reconstituted channels. MIP28 was not phosphorylated by cAMP dependent protein kinase (PKA). Although unitary conductance
and selectivity of the chicken MIP channel are similar to those reported for the bovine MIP (MIP26), the voltage sensitivity
of MIP28 was higher than that of the bovine homologue, and voltage sensitivity of MIP28 was not modulated by treatments previously
shown to affect MIP26 voltage gating (partial proteolysis and protein phosphorylation by PKA: (Ehring et al., 1990). The existence
of such strikingly different functional properties in highly homologous channel isoforms may provide a useful system for exploration
of the structure-function relations of MIP channels.
Received: 27 March 1996/Revised: 5 August 1996 相似文献
23.
We have broadly defined the DNA regions regulating esterase6 activity in
several life stages and tissue types of D. melanogaster using P-
element-mediated transformation of constructs that contain the esterase6
coding region and deletions or substitutions in 5' or 3' flanking DNA.
Hemolymph is a conserved ancestral site of EST6 activity in Drosophila and
the primary sequences regulating its activity lie between -171 and -25 bp
relative to the translation initiation site: deletion of these sequences
decrease activity approximately 20-fold. Hemolymph activity is also
modulated by four other DNA regions, three of which lie 5' and one of which
lies 3' of the coding region. Of these, two have positive and two have
negative effects, each of approximately twofold. Esterase6 activity is
present also in two male reproductive tract tissues; the ejaculatory bulb,
which is another ancestral activity site, and the ejaculatory duct, which
is a recently acquired site within the melanogaster species subgroup.
Activities in these tissues are at least in part independently regulated:
activity in the ejaculatory bulb is conferred by sequences between -273 and
-172 bp (threefold decrease when deleted), while activity in the
ejaculatory duct is conferred by more distal sequences between -844 and
-614 bp (fourfold decrease when deleted). The reproductive tract activity
is further modulated by two additional DNA regions, one in 5' DNA (-613 to
-284 bp; threefold decrease when deleted) and the other in 3' DNA (+1860 to
+2731 bp; threefold decrease when deleted) that probably overlaps the
adjacent esteraseP gene. Collating these data with previous studies
suggests that expression of EST6 in the ancestral sites is mainly regulated
by conserved proximal sequences while more variable distal sequences
regulate expression in the acquired ejaculatory duct site.
相似文献
24.
Prof. Renato Meduri Emilio Campos Lucia Scorolli Caterina De Vinci Giancarlo Pizza Dimitri Viza 《Biotherapy》1996,9(1-3):61-66
Recurrent ocular herpes is an insoluble problem for the clinician. As cellular immunity plays an important role in controlling
herpes relapses, and other studies have shown the efficacy of HSV-specific transfer factor (TF) for the treatment of herpes
patients, an open clinical trial was undertaken in 134 patients (71 keratitis, 29 kerato-uveitis, 34 uveitis) suffering from
recurrent ocular herpetic infections. The mean duration of the treatment was 358 days, and the entire follow-up period 189121
before, and 64062 days after TF treatment. The cell-mediated immune response to the viral antigens, evaluated by the lymphocyte
stimulation test (LST) and the leucocyte migration test (LMT) (P<0.001), was significantly increased by the TF treatment.
The total number of relapses was decreased significantly during/after TF treatment, dropping from 832 before, to 89 after
treatment, whereas the cumulative relapse index (RI) dropped, during the same period, from 13.2 to 4.17 (P<0.0001). No side
effects were observed. It is concluded that patients with relapsing ocular herpes can benefit from treatment with HSV-specific
TF. 相似文献
25.
Examination of 111 peacock wrasse [Symphodus tinea (L.)] and 97 brown wrasse ( Labrus merula L.) from the Valencian coast (Spain) yielded 24 metazoan parasite species (11 Digenea, three Cestoda, four Nematoda. one Acanlhocephala, five Crustacea). Eighteen species were from 5. tinea and 17 from L. merula; 11 of the 24 species were common to both hosts. Brillouin's diversity index, was applied to fully censused parasite infracommunities. This is the first time that all the metazoan parasites (internal and external) in any position in the host have been analysed for diversity. High values of prevalence, intensity, and diversity parameters indicate that these labrid fishes support diverse parasite communities. This may be due to their diverse diet, mainly marine invertebrates which are possible intermediate hosts; they also support certain netnatodes and crustaceans which have direct life-cycle. 相似文献
26.
Elena P. Sawin Laurence R. Harris Ana R. Campos Marla B. Sokolowski 《Journal of Insect Behavior》1994,7(4):553-567
In this paper we examine theDrosophila melanogaster larval response to light. We survey the morphology of the larval visual and motor systems in relation to larval locomotory behavior and phototaxis. In addition, this paper proposes a model of sensorimotor transformation and examines the reversal in taxis occurring at theD. melanogaster larval wnadering stage. 相似文献
27.
Observations on the biology ofCheiropachus quadrum (Hym: Pteromalidae) andDendrosoter protuberans (Hym: Braconidae), were conducted. Both species are the main parasites of the olive bark beetlesHylesinus varius andPhloeotribus scarabaeoides (Col: Scolytidae) in the South of Spain. Results have shown that an increase in body size of the host does not imply an increase in parasite efficiency. In fact, host size inversely affects parasite efficiency forC. quadrum. Bearing in mind this fact, the abundance of the host and the ease of its rearing in the lab, it is therefore advisable to useP. scarabaeoides as the host for mass rearing of the parasites studied here. On the other hand, the presence of white light is a negative factor for parasite longevity and fecundity. The pupae and all larval instars are parasitised.C. quadrum does not have a preference for any particular stage or larval instar of the host whilst there is a preference for the third and fifth larval instar byD. protuberans. With respect to the sex ratio of parasites, an increase in the number of males increases the fecundity of the females. The results obtained in this study can be considered essential in the development of a biological control system for olive bark beetle pests based on an increase in the population ofC. quadrum andD. protuberans. 相似文献
28.
Angel G. Hernandez Ana Rascon Shirley Kutner Henry Roman Zulaika Campos 《Molecular biology reports》1993,18(3):189-195
A correlation between the ratio of the cell surface protease activity to phosphatase activity and the complexity of the pattern of cell surface exposed polypeptides ofLeishmania promastigotes was demonstrated for various strains grown under similar conditions. The ratio of the cell surface protease activity to acid phosphatase activity was high forL. major andL.b. panamensis and it correlates with the expression of a single polypeptide of 63 KDa on their cell surface. Intermediate and lower ratios of these enzymatic activites relate with more complex radio-iodinated patterns: two main bands inL.b. guyanensis (70 and 58 KDa) andL.b. braziliensis (72 and 60 KDa) and three main bands 65, 50, 27 KDa in allL.m. mexicana strains tested. Evidence is presented that the acid phosphatase located on theL.m. mexicana cell surface is not an artifact due to a secondary absorption of the secreted acid phosphatase from the culture medium. These results confirm theLeishmania antigen cell surface heterogeneity. The implications on the biology ofLeishmania and the clinical manifestation of leishmaniasis are discussed. 相似文献
29.
Morphology, ontogeny and histochemistry of secretory trichomes of Geranium robertianum (Geraniaceae)
Geranium robertianum bears three types of glandular uniseriate trichomes which originate from a single protodermal cell and develop through periclinal divisions. Type I trichomes are procumbent and have an oval apical cell, two stalk cells and a basal cell. Type II trichomes are erect and have a pear shaped apical cell, two stalk cells and a basal cell. Type III trichomes are much longer than the other two types and have an elongated apical cell, five long stalk cells and a basal cell. Type I and type II trichomes are common on leaves while III trichomes are more abundant on flower structures.
Type I and type II trichomes secrete terpenoids and phenols. Type III trichomes are characterized by the accumulation of anthocyanins in the apical cell and secrete flavonoids. 相似文献
Type I and type II trichomes secrete terpenoids and phenols. Type III trichomes are characterized by the accumulation of anthocyanins in the apical cell and secrete flavonoids. 相似文献
30.
F Torrens A Campos C Abad 《Cellular and molecular biology, including cyto-enzymology》2003,49(6):991-998
The association of poly2-vinylpyridine (P2VPy) and poly4-vinylpyridine (P4VPy) to dimyristoylphosphatidic acid (DMPA) small unilamellar vesicles (SUVs) was studied as a function of pH, ionic strength (I), polymer concentration and temperature using spectrofluorimetry. Poly(vinylpyridine) (PVPy) data were transformed into association isotherms and analyzed in terms of binding and partition models. In the case of polyions, the inclusion of the activity coefficient in both models was essential. Moreover, a relating equation was proposed to compare parameters based on both theoretical approaches. On the basis of the results obtained, a model was developed to analyze polymer adsorption at the surface level, in which the length of the hydrophobic chain and the position of the N atom in the pyridinium ring play an important role. Transition temperature (Tc) for DMPA (ca. 55 degrees C) is decreased between 15 degrees C-19 degrees C in the presence of PVPy. Van't Hoff isochore showed that the binding constant (KA) accounted for average PVPy-DMPA two-dimensional solid and liquid interactions. KA decreased with I in the presence of both polymers, but was more sensitive to I in the case of P2VPy. Likewise, the number of phospholipid heads (N) involved in the binding process decreased with I in the presence of PVPy. The influence of I was more significant on N than on KA. 相似文献