全文获取类型
收费全文 | 2978篇 |
免费 | 201篇 |
出版年
2023年 | 2篇 |
2022年 | 15篇 |
2021年 | 30篇 |
2020年 | 15篇 |
2019年 | 23篇 |
2018年 | 32篇 |
2017年 | 34篇 |
2016年 | 58篇 |
2015年 | 99篇 |
2014年 | 95篇 |
2013年 | 210篇 |
2012年 | 211篇 |
2011年 | 210篇 |
2010年 | 133篇 |
2009年 | 128篇 |
2008年 | 198篇 |
2007年 | 182篇 |
2006年 | 201篇 |
2005年 | 193篇 |
2004年 | 197篇 |
2003年 | 183篇 |
2002年 | 180篇 |
2001年 | 35篇 |
2000年 | 26篇 |
1999年 | 32篇 |
1998年 | 37篇 |
1997年 | 39篇 |
1996年 | 40篇 |
1995年 | 38篇 |
1994年 | 28篇 |
1993年 | 32篇 |
1992年 | 28篇 |
1991年 | 22篇 |
1990年 | 18篇 |
1989年 | 20篇 |
1988年 | 8篇 |
1987年 | 14篇 |
1986年 | 10篇 |
1985年 | 9篇 |
1984年 | 14篇 |
1983年 | 10篇 |
1982年 | 14篇 |
1981年 | 24篇 |
1980年 | 10篇 |
1978年 | 13篇 |
1977年 | 6篇 |
1976年 | 7篇 |
1975年 | 6篇 |
1962年 | 2篇 |
1961年 | 3篇 |
排序方式: 共有3179条查询结果,搜索用时 937 毫秒
121.
Transitory perturbations in the level of cytosolic Ca2+ are well known to be involved in numerous cell signaling pathways in both plant and animal systems. However, not much is known at present about the molecular identity of plant plasma membrane Ca2+ conducting ion channels or their specific roles in signal transduction cascades. A recent study employing genetic approaches as well as patch clamp electrophysiological analysis of channel currents has provided the first such direct evidence linking a specific gene product with inward Ca2+ currents across the plant cell membrane. This work identified Ca2+ permeation through (Arabidopsis) cyclic nucleotide gated channel isoform 2 (CNGC2) as contributing to the plant innate immunity signaling cascade initiated upon perception of a pathogen. Here, we expand on the implications of CNGC2 mediated cytosolic Ca2+ elevations associated with plant cell response to pathogen recognition, and propose some additional steps that may be involved in the innate immunity signal cascade.Key Words: calcium, CNGC, hypersensitive response, nitric oxide, plant innate immunity, plant ion channel, reactive oxygen species 相似文献
122.
Enoki N Kiyoshima T Sakai T Kobayashi I Takahashi K Terada Y Sakai H 《Journal of molecular histology》2007,38(4):321-332
This study investigated the age-dependent changes in the number of BrdU- and TUNEL-positive cells in murine gingival tissue
and submandibular gland, and compared the findings with those in other tissues and organs. The cell proliferative activity
was decreased after 20 weeks of age in epithelial cells of the gingiva, tongue, buccal mucosa and skin. A decreased cell proliferative
activity was also associated with aging in the liver and kidney parenchymal cells. Meanwhile, cell death showed peculiar changes
in gingival subepithelial tissue, and mucous and serous acini of the submandibular gland. An increase of TUNEL-positive cells
was demonstrated in gingival subepithelial tissue after 20-week-old of age. A significant increase of TUNEL-positive cells
was also found in the mucous acinar cells in the 20-week-old mice and in the serous acini after 20 weeks. The fluctuation
in the number of TUNEL-positive cells in the subepithelial tissue of the skin, and BrdU- and TUNEL-positive staining ratios
in the liver was smaller than that in other tissue and organs throughout life. This study may provide useful information for
better understanding the influence of aging on the functional alteration that occurs in the gingival tissue and submandibular
gland of the elderly. 相似文献
123.
Kiyosaki T Matsumoto I Asakura T Funaki J Kuroda M Misaka T Arai S Abe K 《The FEBS journal》2007,274(8):1908-1917
We cloned a new cysteine proteinase of wheat seed origin, which hydrolyzed the storage protein gliadin almost specifically, and was named gliadain. Gliadain mRNA was expressed 1 day after the start of seed imbibition, and showed a gradual increase thereafter. Gliadain expression was suppressed when uniconazol, a gibberellin synthesis inhibitor, was added to germinating seeds. Histochemical detection with anti-gliadain serum indicated that gliadain was present in the aleurone layer and also that its expression intensity increased in sites nearer the embryo. The enzymological characteristics of gliadain were investigated using recombinant glutathione S-transferase (GST)-progliadain fusion protein produced in Escherichia coli. The GST-progliadain almost specifically digested gliadin into low molecular mass peptides. These results indicate that gliadain is produced via gibberellin-mediated gene activation in aleurone cells and secreted into the endosperm to digest its storage proteins. Enzymologically, the GST-progliadain hydrolyzed benzyloxycarbonyl-Phe-Arg-7-amino-4-methylcoumarin (Z-Phe-Arg-NH(2)-Mec) at K(m) = 9.5 microm, which is equivalent to the K(m) value for hydrolysis of this substrate by cathepsin L. Hydrolysis was inhibited by two wheat cystatins, WC1 and WC4, with IC(50) values of 1.7 x 10(-8) and 5.0 x 10(-8) m, respectively. These values are comparable with those found for GST-progliadain inhibition by E-64 and egg-white cystatin, and are consistent with the possibility that, in germinating wheat seeds, gliadain is under the control of intrinsic cystatins. 相似文献
124.
125.
Adrienne H K Roeder Marisa S Otegui Ram Dixit Charles T Anderson Christine Faulkner Yan Zhang Maria J Harrison Charlotte Kirchhelle Gohta Goshima Jeremy E Coate Jeff J Doyle Olivier Hamant Keiko Sugimoto Liam Dolan Heather Meyer David W Ehrhardt Arezki Boudaoud Carlos Messina 《The Plant cell》2022,34(1):72
As scientists, we are at least as excited about the open questions—the things we do not know—as the discoveries. Here, we asked 15 experts to describe the most compelling open questions in plant cell biology. These are their questions: How are organelle identity, domains, and boundaries maintained under the continuous flux of vesicle trafficking and membrane remodeling? Is the plant cortical microtubule cytoskeleton a mechanosensory apparatus? How are the cellular pathways of cell wall synthesis, assembly, modification, and integrity sensing linked in plants? Why do plasmodesmata open and close? Is there retrograde signaling from vacuoles to the nucleus? How do root cells accommodate fungal endosymbionts? What is the role of cell edges in plant morphogenesis? How is the cell division site determined? What are the emergent effects of polyploidy on the biology of the cell, and how are any such “rules” conditioned by cell type? Can mechanical forces trigger new cell fates in plants? How does a single differentiated somatic cell reprogram and gain pluripotency? How does polarity develop de-novo in isolated plant cells? What is the spectrum of cellular functions for membraneless organelles and intrinsically disordered proteins? How do plants deal with internal noise? How does order emerge in cells and propagate to organs and organisms from complex dynamical processes? We hope you find the discussions of these questions thought provoking and inspiring.We asked 15 experts to address what they consider to be the most compelling open questions in plant cell biology and these are their questions. 相似文献
126.
127.
A variety of cytokines have been reported to be able to recognize specific carbohydrate moieties. To date, the role of carbohydrate recognition in cytokine function has been analyzed for several cytokines, including fibroblast growth factor (FGF), tumor necrosis factor (TNF)-alpha, and interleukin (IL)-2. The FGF family and their receptors have been found to recognize a heparan sulfate proteoglycan, which generates rigid complexes that induce signal transduction. We have found that IL-2 recognizes a high-mannose type glycan on the alpha subunit of the IL-2 receptor as well as a peptide portion of this subunit. Blocking this carbohydrate-IL-2 interaction diminished IL-2-induced signaling and T-cell proliferation. We have also shown that TNF-alpha recognizes the second mannose 6-phosphate diester of the glycan portion of glycosylphosphatidylinositol (GPI)-anchored glycoproteins. Blocking this GPI-anchored glycan-TNF-alpha interaction abrogates TNF-alpha-induced apoptosis. We aim to increase the number of cytokines which modulate their functions through the unique carbohydrate recognition, and open the way to systematically elucidate the biological functions of cytokine-carbohydrate interaction in immune system. 相似文献
128.
Inactivation of Stat5 in mouse mammary epithelium during pregnancy reveals distinct functions in cell proliferation, survival, and differentiation 总被引:19,自引:0,他引:19
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Cui Y Riedlinger G Miyoshi K Tang W Li C Deng CX Robinson GW Hennighausen L 《Molecular and cellular biology》2004,24(18):8037-8047
129.
Nishijo M Satarug S Honda R Tsuritani I Aoshima K 《Molecular and cellular biochemistry》2004,255(1-2):87-92
On a viewpoint of gender differences in Cd body burden and its health effects, we reviewed the population-based research including our own which conducted in Japan, Thailand, Australia, Poland, Belgium and Sweden to assess health effects of human exposure to environmental cadmium and their potential mechanisms. As a result, six risk factors in Cd health effects in women have been identified; (1) more serious type of renal tubular dysfunction, (2) difference in calcium metabolism and its regulatory hormones, (3) kidney sensitivity; difference in P450 phenotype, (4) pregnancy, (5) body iron store status, and (6) genetic factors. Further studies of Cd toxicity targeted to women would now appear necessary. 相似文献
130.
Local delivery of soluble TNF-alpha receptor 1 gene reduces infarct size following ischemia/reperfusion injury in rats 总被引:14,自引:0,他引:14
Sugano M Hata T Tsuchida K Suematsu N Oyama J Satoh S Makino N 《Molecular and cellular biochemistry》2004,266(1-2):127-132
Apoptosis in the myocardium is linked to ischemia/reperfusion injury, and TNF-alpha induces apoptosis in cardiomyocytes. A significant amount of TNF-alpha is detected after ischemia and reperfusion. Soluble TNF-alpha receptor 1 (sTNFR1) is an extracellular domain of TNF-alpha receptor 1 and is an antagonist to TNF-alpha. In the present study, we examined the effects of sTNFR1 on infarct size in acute myocardial infarction (AMI) following ischemia/reperfusion. Male Wistar rats were subjected to left coronary artery (LCA) ligation. After 30 min of LCA occlusion, the temporary ligature on the LCA was released and blood flow was restored. Immediately after reperfusion, a total of 200 microg of sTNFR1 or LacZ plasmid was injected into three different sites of the left ventricular wall. At 6 h, 1 and 2 days after reperfusion, the TNF-alpha bioactivity in the myocardium was significantly higher in rats receiving LacZ plasmid than in sham-operated rats, whereas sTNFR1 plasmid significantly suppressed the increase in the TNF-alpha bioactivity. The sTNFR1 plasmid significantly reduced DNA fragmentation and caspase activity compared to the LacZ plasmid. Finally, the sTNFR1 expression-plasmid treatment significantly reduced the area of myocardial infarction at 2 days after ischemia/reperfusion compared to LacZ plasmid. In conclusion, the TNF-alpha bioactivity in the heart increased from the early stage of ischemia/reperfusion, and this increase was thought to contribute in part to the increased area of myocardial infarction. Suppression of TNF-alpha bioactivity with the sTNFR1 plasmid reduced the infarct size in AMI following ischemia and reperfusion. 相似文献