全文获取类型
收费全文 | 1448篇 |
免费 | 74篇 |
出版年
2022年 | 4篇 |
2021年 | 13篇 |
2020年 | 6篇 |
2019年 | 10篇 |
2018年 | 16篇 |
2017年 | 16篇 |
2016年 | 21篇 |
2015年 | 38篇 |
2014年 | 41篇 |
2013年 | 109篇 |
2012年 | 89篇 |
2011年 | 102篇 |
2010年 | 65篇 |
2009年 | 61篇 |
2008年 | 112篇 |
2007年 | 100篇 |
2006年 | 90篇 |
2005年 | 76篇 |
2004年 | 109篇 |
2003年 | 100篇 |
2002年 | 92篇 |
2001年 | 9篇 |
2000年 | 15篇 |
1999年 | 17篇 |
1998年 | 18篇 |
1997年 | 20篇 |
1996年 | 20篇 |
1995年 | 18篇 |
1994年 | 10篇 |
1993年 | 9篇 |
1992年 | 11篇 |
1991年 | 10篇 |
1990年 | 10篇 |
1989年 | 3篇 |
1988年 | 8篇 |
1987年 | 7篇 |
1986年 | 6篇 |
1985年 | 3篇 |
1984年 | 6篇 |
1983年 | 7篇 |
1982年 | 5篇 |
1980年 | 11篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1975年 | 4篇 |
1974年 | 3篇 |
1972年 | 2篇 |
1962年 | 1篇 |
排序方式: 共有1522条查询结果,搜索用时 565 毫秒
901.
VCIP135, a novel essential factor for p97/p47-mediated membrane fusion,is required for Golgi and ER assembly in vivo 总被引:8,自引:0,他引:8
Uchiyama K Jokitalo E Kano F Murata M Zhang X Canas B Newman R Rabouille C Pappin D Freemont P Kondo H 《The Journal of cell biology》2002,159(5):855-866
NSF and p97 are ATPases required for the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of organelles. NSF uses ATP hydrolysis to dissociate NSF/SNAPs/SNAREs complexes, separating the v- and t-SNAREs, which are then primed for subsequent rounds of fusion. In contrast, p97 does not dissociate the p97/p47/SNARE complex even in the presence of ATP. Now we have identified a novel essential factor for p97/p47-mediated membrane fusion, named VCIP135 (valosin-containing protein [VCP][p97]/p47 complex-interacting protein, p135), and show that it binds to the p97/p47/syntaxin5 complex and dissociates it via p97 catalyzed ATP hydrolysis. In living cells, VCIP135 and p47 are shown to function in Golgi and ER assembly. 相似文献
902.
Protective effects of in vivo‐expressed autotransporters against Bordetella pertussis infection
下载免费PDF全文
![点击此处可从《Microbiology and immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Koichiro Suzuki Naoaki Shinzawa Keisuke Ishigaki Keiji Nakamura Hiroyuki Abe Aya Fukui‐Miyazaki Kazuyoshi Ikuta Yasuhiko Horiguchi 《Microbiology and immunology》2017,61(9):371-379
903.
Colvin KM Gordon VD Murakami K Borlee BR Wozniak DJ Wong GC Parsek MR 《PLoS pathogens》2011,7(1):e1001264
Bacterial extracellular polysaccharides are a key constituent of the extracellular matrix material of biofilms. Pseudomonas aeruginosa is a model organism for biofilm studies and produces three extracellular polysaccharides that have been implicated in biofilm development, alginate, Psl and Pel. Significant work has been conducted on the roles of alginate and Psl in biofilm development, however we know little regarding Pel. In this study, we demonstrate that Pel can serve two functions in biofilms. Using a novel assay involving optical tweezers, we demonstrate that Pel is crucial for maintaining cell-to-cell interactions in a PA14 biofilm, serving as a primary structural scaffold for the community. Deletion of pelB resulted in a severe biofilm deficiency. Interestingly, this effect is strain-specific. Loss of Pel production in the laboratory strain PAO1 resulted in no difference in attachment or biofilm development; instead Psl proved to be the primary structural polysaccharide for biofilm maturity. Furthermore, we demonstrate that Pel plays a second role by enhancing resistance to aminoglycoside antibiotics. This protection occurs only in biofilm populations. We show that expression of the pel gene cluster and PelF protein levels are enhanced during biofilm growth compared to liquid cultures. Thus, we propose that Pel is capable of playing both a structural and a protective role in P. aeruginosa biofilms. 相似文献
904.
905.
Symbiotic association in Chlorella culture 总被引:2,自引:0,他引:2
Watanabe K Takihana N Aoyagi H Hanada S Watanabe Y Ohmura N Saiki H Tanaka H 《FEMS microbiology ecology》2005,51(2):187-196
Chlorella sorokiniana IAM C-212 has long been maintained in slant culture as a mixed strain, representing an associated natural microbial consortium. In this study, the consortium was separated and five nonalgal constituents, a fungal strain (CSSF-1), and four bacterial strains (CSSB-1, CSSB-2, CSSB-3, and CSSB-4) were isolated and identified. 16S rDNA sequence analysis revealed that strains CSSB-1, CSSB-2, CSSB-3, and CSSB-4 were close to Ralstonia pickettii (99.8% identity), Sphingomonas sp. DD38 (99.4% identity), Microbacterium trichotecenolyticum (98.6% identity), and Micrococcus luteus (98.6% identity) respectively. 18S rDNA sequence analysis revealed that strain CSSF-1 resembled Acremonium-like hyphomycete KR21-2 (98.8%). The fungal strain CSSF-1 and one of the bacterial strains, CSSB-3, were found to promote the growth of Chlorella while the presence of bacterial strains CSSB-1 and CSSB-2 had no effect. Strain CSSB-4 could not be subcultured so its role was not elucidated. These results show that the interaction between Chlorella and its symbionts under photoautotrophic conditions involved both mutualism and commensalisms. The chlorophyll content of mixed strain was stable in long-term cultivation (7 months) while the chlorophyll content of a pure culture showed a marked decline. Electron microscopic analysis showed the two bacterial strains CSSB-2 and CSSB-3 were harbored on the sheath excreted by Chlorella, while the fungal strain CSSF-1 and the bacterial strain CSSB-1 directly adhered to the Chlorella cell surface. This report is the first observation of a symbiotic relationship among fungus, bacteria, and Chlorella, and the first observation of direct adhesion of fungus and bacteria to Chlorella in a consortium. 相似文献
906.
907.
Keiji Uehara Toshimasa Harumoto Asana Makino Yasuo Koda Junko Iwano Yasuhiro Suzuki Mari Tanigawa Hiroto Iwai Kana Asano Kana Kurihara Akinori Hamaguchi Hiroshi Kodaira Toshiyuki Atsumi Yoji Yamada Kazuma Tomizuka 《Nucleic acids research》2022,50(9):4840
Extrahepatic delivery of small interfering RNAs (siRNAs) may have applications in the development of novel therapeutic approaches. However, reports on such approaches are limited, and the scarcity of reports concerning the systemically targeted delivery of siRNAs with effective gene silencing activity presents a challenge. We herein report for the first time the targeted delivery of CD206-targetable chemically modified mannose–siRNA (CMM–siRNA) conjugates to macrophages and dendritic cells (DCs). CMM–siRNA exhibited a strong binding ability to CD206 and selectively delivered contents to CD206-expressing macrophages and DCs. Furthermore, the conjugates demonstrated strong gene silencing ability with long-lasting effects and protein downregulation in CD206-expressing cells in vivo. These findings could broaden the use of siRNA technology, provide additional therapeutic opportunities, and establish a basis for further innovative approaches for the targeted delivery of siRNAs to not only macrophages and DCs but also other cell types. 相似文献
908.
Jumpei Uchiyama Hiroaki Takeuchi Shin-ichiro Kato Keiji Gamoh Iyo Takemura-Uchiyama Takako Ujihara Masanori Daibata Shigenobu Matsuzaki 《Applied and environmental microbiology》2013,79(10):3176-3184
Helicobacter pylori inhabits the stomach mucosa and is a causative agent of stomach ulcer and cancer. In general, bacteriophages (phages) are strongly associated with bacterial evolution, including the development of pathogenicity. Several tailed phages have so far been reported in H. pylori. We have isolated an H. pylori phage, KHP30, and reported its genomic sequence. In this study, we examined the biological characteristics of phage KHP30. Phage KHP30 was found to be a spherical lipid-containing phage with a diameter of ca. 69 nm. Interestingly, it was stable from pH 2.5 to pH 10, suggesting that it is adapted to the highly acidic environment of the human stomach. Phage KHP30 multiplied on 63.6% of clinical H. pylori isolates. The latent period was ca. 140 min, shorter than the doubling time of H. pylori (ca. 180 min). The burst size was ca. 13, which was smaller than the burst sizes of other known tailed or spherical phages. Phage KHP30 seemed to be maintained as an episome in H. pylori strain NY43 cells, despite a predicted integrase gene in the KHP30 genomic sequence. Seven possible virion proteins of phage KHP30 were analyzed using N-terminal protein sequencing and mass spectrometry, and their genes were found to be located on its genomic DNA. The genomic organization of phage KHP30 differed from the genomic organizations in the known spherical phage families Corticoviridae and Tectiviridae. This evidence suggests that phage KHP30 is a new type of spherical phage that cannot be classified in any existing virus category. 相似文献
909.
910.