全文获取类型
收费全文 | 7365篇 |
免费 | 745篇 |
国内免费 | 1篇 |
专业分类
8111篇 |
出版年
2022年 | 60篇 |
2021年 | 134篇 |
2020年 | 72篇 |
2019年 | 100篇 |
2018年 | 99篇 |
2017年 | 85篇 |
2016年 | 158篇 |
2015年 | 239篇 |
2014年 | 268篇 |
2013年 | 326篇 |
2012年 | 436篇 |
2011年 | 410篇 |
2010年 | 259篇 |
2009年 | 216篇 |
2008年 | 333篇 |
2007年 | 322篇 |
2006年 | 339篇 |
2005年 | 279篇 |
2004年 | 319篇 |
2003年 | 281篇 |
2002年 | 289篇 |
2001年 | 176篇 |
2000年 | 177篇 |
1999年 | 148篇 |
1998年 | 85篇 |
1997年 | 77篇 |
1996年 | 89篇 |
1995年 | 60篇 |
1994年 | 64篇 |
1993年 | 74篇 |
1992年 | 98篇 |
1991年 | 110篇 |
1990年 | 111篇 |
1989年 | 81篇 |
1988年 | 93篇 |
1987年 | 90篇 |
1986年 | 94篇 |
1985年 | 88篇 |
1984年 | 63篇 |
1983年 | 56篇 |
1982年 | 64篇 |
1981年 | 67篇 |
1980年 | 49篇 |
1979年 | 65篇 |
1978年 | 71篇 |
1977年 | 51篇 |
1976年 | 43篇 |
1975年 | 49篇 |
1973年 | 57篇 |
1971年 | 41篇 |
排序方式: 共有8111条查询结果,搜索用时 0 毫秒
991.
Several authors have suggested that the consumption of plant compounds may have direct effects on wild primate reproductive biology, but no studies have presented physiological evidence of such effects. Here, for two troops of olive baboons (Papio hamadryas anubis) at Gashaka-Gumti National Park, Nigeria, we show major seasonal increases in levels of fecal progesterone metabolites in females, and provide evidence that this is linked to the consumption of natural plant compounds. Increases in fecal progestogen excretion occurred seasonally in all females, in all reproductive states, including lactation. Detailed feeding data on the study animals showed that only one food species is consumed by both troops at the time of observed progestogen peaks, and at no other times of the year: the African black plum, Vitex doniana. Laboratory tests demonstrated the presence of high concentrations of progestogen-like compounds in V. doniana. Together with published findings linking the consumption of a related Vitex species (Vitex agnus castus) to increased progestogen levels in humans, our data suggest that natural consumption of V. doniana was a likely cause of the observed increases in progestogens. Levels of progestogen excretion in the study baboons during periods of V. doniana consumption are higher than those found during pregnancy, and prevent the expression of the sexual swelling, which is associated with ovulatory activity. As consortship and copulatory activity in baboons occur almost exclusively in the presence of a sexual swelling, V. doniana appears to act on cycling females as both a physiological contraceptive (simulating pregnancy in a similar way to some forms of the human contraceptive pill) and a social contraceptive (preventing sexual swelling, thus reducing association and copulation with males). The negative effects of V. doniana on reproduction may be counter-balanced by the wide-range of medicinal properties attributed to plants in this genus. This is the first time that physiological evidence has been presented of direct effects of plant consumption on the reproductive biology of wild primates. 相似文献
992.
Escherichia coli K1-specific bacteriophage CUS-3 distribution and function in phase-variable capsular polysialic acid O acetylation 下载免费PDF全文
King MR Vimr RP Steenbergen SM Spanjaard L Plunkett G Blattner FR Vimr ER 《Journal of bacteriology》2007,189(17):6447-6456
Escherichia coli K1 is the leading cause of human neonatal sepsis and meningitis and is important in other clinical syndromes of both humans and domestic animals; in this strain the polysialic acid capsule (K1 antigen) functions by inhibiting innate immunity. Recent discovery of the phase-variable capsular O acetylation mechanism indicated that the O-acetyltransferase gene, neuO, is carried on a putative K1-specific prophage designated CUS-3 (E. L. Deszo, S. M. Steenbergen, D. I. Freedberg, and E. R. Vimr, Proc. Natl. Acad. Sci. USA 102:5564-5569, 2005). Here we describe the isolation and characterization of a CUS-3 derivative (CUS-3a), demonstrating its morphology, lysogenization of a sensitive host, and the distribution of CUS-3 among a collection of 111 different K1 strains. The 40,207-bp CUS-3 genome was annotated from the strain RS218 genomic DNA sequence, indicating that most of the 63 phage open reading frames have their closest homologues in one of seven different lambdoid phages. Translational fusion of a reporter lacZ fragment to the hypervariable poly-Psi domain facilitated measurement of phase variation frequencies, indicating no significant differences between switch rates or effects on rates of the methyl-directed mismatch repair system. PCR analysis of poly-Psi domain length indicated preferential loss or gain of single 5'-AAGACTC-3' nucleotide repeats. Analysis of a K1 strain previously reported as "locked on" indicated a poly-Psi region with the least number of heptad repeats compatible with in-frame neuO expression. The combined results establish CUS-3 as an active mobile contingency locus in E. coli K1, indicating its capacity to mediate population-wide capsule variation. 相似文献
993.
Abandoning aggression but maintaining self-nonself discrimination as a first stage in ant supercolony formation 总被引:2,自引:0,他引:2
Steiner FM Schlick-Steiner BC Moder K Stauffer C Arthofer W Buschinger A Espadaler X Christian E Einfinger K Lorbeer E Schafellner C Ayasse M Crozier RH 《Current biology : CB》2007,17(21):1903-1907
An ant supercolony is a very large entity with very many queens. Although normal colonies of small extent and few queens remain distinct, a supercolony is integrated harmoniously over a large area [1, 2]. The lack of aggression is advantageous: Aggression is costly, involving direct and indirect losses and recognition errors [3, 4]. Indeed, supercolonial ants are among the ecologically most successful organisms [5-7]. But how supercolonies arise remains mysterious [1, 2, 8]. Suggestions include that reduced within-colony relatedness or reduced self-nonself discrimination would foster supercolony formation [1, 2, 5, 7, 9-12]. However, one risks confusing correlation and causality in deducing the evolution from distinct colonies to supercolonies when observing established supercolonies. It might help to follow up observations of another lack of aggression, that between single-queened colonies in some ant species. We show that the single-queened Lasius austriacus lacks aggression between colonies and occasionally integrates workers across colonies but maintains high within-colony relatedness and self-nonself discrimination. Provided that the ecological framework permits, reduced aggression might prove adaptive for any ant colony irrespective of within-colony relatedness. Abandoning aggression while maintaining discrimination might be a first stage in supercolony formation. This adds to the emphasis of ecology as central to the evolution of cooperation in general [13]. 相似文献
994.
995.
Bilirubin as an antioxidant in micelles and lipid bilayers: its contribution to the total antioxidant capacity of human blood plasma 总被引:1,自引:0,他引:1
The antioxidant capacities, antioxidant activities, k(inh), and stoichiometric factors, n, of water-soluble derivatives of bilirubin (BR), BR-human serum albumin (BR-HSA), and BR-ditaurate disodium conjugate (BRC) were determined in aqueous/lipid dispersions of sodium dodecyl sulfate (SDS) micelles/methyl linoleate and in bilayers of dilinoleoylphosphatidylcholine (DLPC) during initiation by water-soluble azo-bis-amidinopropane dihydrochloride (ABAP). The inhibition rate constants for BRC and BR-HSA were similar in micelles (k(inh) approximately 1.3 x 10(4) M(-1) s(-1)), where n approximately 2, whereas the k(inh) for BR-HSA dropped by (1/2) in bilayers. The dimethyl ester of bilirubin (BRDE) gave a k(inh) only one-tenth that of the vitamin E analog, pentamethylhydroxychroman (PMHC) in SDS micelles/methyl linoleate when initiated by lipid-soluble azo-bis-2,4-dimethylvaleronitrile (DMVN). Biliverdin hydrochloride (BVHCl) was NOT an effective peroxyl radical-trapping agent in the micellar phase during initiation by ABAP or DMVN containing methyl linoleate but it inhibited oxygen uptake in the aqueous phase. Both BRC and BR-HSA extended the total radical antioxidant parameter (TRAP) of human blood plasma and their contribution to TRAP was in the range of 5-10% of the natural TRAP of blood plasma, depending on the BR content determined in the blood plasma. 相似文献
996.
The aim of this work was to investigate the influence of some non-ionic surfactants, Tween 80 and Brij 98, on the viscosity
and flow behavior of a commercial montmorillonite clay, Veegum Granules. The effect of different concentrations of the surfactants
on the shear stress-shear rate rheograms of hydrated concentrated clay suspensions was determined by shear viscometry. The
addition of either surfactant increased the plastic viscosity and the yield stress of the suspensions. Furthermore both surfactants
altered the thixotropy of the suspensions to an extent that depended on both the surfactant concentration and the time of
equilibration of the surfactant and Veegum. Brij 98 had a greater and more rapid effect. It is proposed that the surfactant
polar head-groups anchor at the tetrahedral sheet surface, leaving the alkyl chains extending away from the edges and faces.
Consequently, the alkyl chains undergo hydrophobic interactions that facilitate the association between the platelets and
increase the physical structure within the suspension. Stereochemical differences between the polar groups may lead to differences
in the way the surfactants associate with the tetrahedral sheet and hence their ultimate effect on the rheological behavior.
There is a significant interaction between these surfactants and montmorillonite clays, and the rheological changes that occur
could have a major impact on any pharmaceutical formulation that uses these ingredients.
Published: March 30, 2007 相似文献
997.
Biodiversity and Conservation - Invasive predators, land clearing and altered fire regimes have been implicated in species decline and extinction worldwide. Enhanced knowledge of how these factors... 相似文献
998.
Identifying the contributions to thermodynamic stability of capsids is of fundamental and practical importance. Here we use simulation to assess how mutations affect the stability of lumazine synthase from the hyperthermophile Aquifex aeolicus, a T = 1 icosahedral capsid; in the simulations the icosahedral symmetry of the capsid is preserved by simulating a single pentamer and imposing crystal symmetry, in effect simulating an infinite cubic lattice of icosahedral capsids. The stability is assessed by estimating the free energy of association using an empirical method previously proposed to identify biological units in crystal structures. We investigate the effect on capsid formation of seven mutations, for which it has been experimentally assessed whether they disrupt capsid formation or not. With one exception, our approach predicts the effect of the mutations on the capsid stability. The method allows the identification of interaction networks, which drive capsid assembly, and highlights the plasticity of the interfaces between subunits in the capsid. Proteins 2015; 83:1733–1741. © 2015 The Authors. Proteins: Structure, Function, and Bioinformatics Published by Wiley Periodicals, Inc 相似文献
999.
1000.