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351.
Establishing a core microbiome is the first step in understanding and subsequently optimizing microbial interactions in anodic biofilms of microbial fuel cells (MFCs) for increased power, efficiency, and decreased start-up times. In the present study, we used 454 pyrosequencing to demonstrate that a core anodic community would consistently emerge over a period of 4 years given similar conditions. The development and variation across reactor designs of these communities was also explored. The core members present in all high-power generating biofilms were Geobacter, Aminiphilus, Sedimentibacter, Acetoanaerobium, and Spirochaeta, accounting for 72?±?9 % of all genera. Aminiphilus spp., member of the Synergistetes phylum was present at higher abundances than previously reported in any other ecological studies. Results suggest a stable core microbiome in acetate-fed MFCs on both phylogenetic and functional levels. 相似文献
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Edward J. Pavlik Daniel E. Kenady John R. van Nagell Kathryn Keaton Elvis S. Donaldson Michael B. Hanson Robert C. Flanigan 《In vitro cellular & developmental biology. Plant》1983,19(7):538-550
Summary Human tumor cell lines, derived from cancers of the colon, ovary, and cervix, were grown in liquid tissue culture media and
media made semisolid with agar (Bacto & deoxycholate lactose agar), agarose [LE, ME, Sea Plaque and Sea Prep (15/45)], and
methyl cellulose. The effects of each agent on overall cell proliferation and rate of overall cell proliferation were examined.
The agents, used to make media semisolid, were observed to inhibit or, in some cases, enhance cell growth in a fashion that
was characteristic of individual cell lines. These phenomena may be of consequence to the optimization of nutrient media for
primary tumor cell preparations.
This work was supported by the Veteran's Administration. 相似文献