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91.
Brendan K. Hobart H. Anu Kramer Gavin M. Jones Brian P. Dotters Sheila A. Whitmore John J. Keane M. Zachariah Peery 《Ibis》2021,163(1):253-259
Although the effects of shifting fire regimes on bird populations have been recognized as important to ecology and conservation, the consequences of fire for trophic interactions of avian species – and raptors in particular – remain relatively unknown. Here, we found that within national parks with long‐standing (40+ years) fire management programmes, California Spotted Owls Strix occidentalis occidentalis consumed predominantly Woodrats Neotoma spp. and Pocket Gophers Thomomys spp.; however, in contrast to our predictions, when their territories experienced more extensive and frequent fire, Spotted Owls consumed proportionally more Flying Squirrels Glaucomys oregonensis. We hypothesize this finding could have been driven by either changes to prey abundance following fires (e.g. increases in flying squirrels) or changes to prey availability (e.g. shifts in forest structure or flying squirrel spatial distribution that increased predation upon them by owls). Our work thus demonstrates that fire may have unexpected consequences for the trophic interactions of raptor species and provides valuable information for the conservation of Spotted Owls in fire‐prone forest landscapes. 相似文献
92.
Ball N Nolan E Wheeler K 《Journal of strength and conditioning research / National Strength & Conditioning Association》2011,25(10):2752-2763
Physiological, anthropometric, and power profiling data were retrospectively analyzed from 4 elite taekwondo athletes from the Australian National Olympic team 9 weeks from Olympic departure. Power profiling data were collected weekly throughout the 9-week period. Anthropometric skinfolds generated a lean mass index (LMI). Physiological tests included a squat jump and bench throw power profile, bleep test, 20-m sprint test, running VO2max test, and bench press and squat 3 repetition maximum (3RM) strength tests. After this, the athletes power, velocity, and acceleration profile during unweighted squat jumps and single-leg jumps were tracked using a linear position transducer. Increases in power, velocity, and acceleration between weeks and bilateral comparisons were analyzed. Athletes had an LMI of 37.1 ± 0.4 and were 173.9 ± 0.2 m and 67 ± 1.1 kg. Relatively weaker upper body (56 ± 11.97 kg 3RM bench press) compared to lower body strength (88 ± 2.89 kg 3RM squat) was shown alongside a VO2max of 53.29 ml(-1)·min(-1)·kg, and a 20-m sprint time of 3.37 seconds. Increases in all power variables for single-leg squat and squat jumps were found from the first session to the last. Absolute peak power in single-leg squat jumps increased by 13.4-16% for the left and right legs with a 12.9% increase in squat jump peak power. Allometrically scaled peak power showed greater increases for single-leg (right leg: 18.55%; left: 23.49%) and squat jump (14.49%). The athlete's weight did not change significantly throughout the 9-week mesocycle. Progressions in power increases throughout the weeks were undulating and can be related to the intensity of the prior week's training and athlete injury. This analysis has shown that a 9-week mesocycle before Olympic departure that focuses on core lifts has the ability to improve power considerably. 相似文献
93.
94.
Fibroblast activation protein-α (FAP) is a cell surface-expressed and soluble enzyme of the prolyl oligopeptidase family, which includes dipeptidyl peptidase 4 (DPP4). FAP is not generally expressed in normal adult tissues, but is found at high levels in activated myofibroblasts and hepatic stellate cells in fibrosis and in stromal fibroblasts of epithelial tumours. FAP possesses a rare catalytic activity, hydrolysis of the post-proline bond two or more residues from the N-terminus of target substrates. α(2)-antiplasmin is an important physiological substrate of FAP endopeptidase activity. This study reports the first natural substrates of FAP dipeptidyl peptidase activity. Neuropeptide Y, B-type natriuretic peptide, substance P and peptide YY were the most efficiently hydrolysed substrates and the first hormone substrates of FAP to be identified. In addition, FAP slowly hydrolysed other hormone peptides, such as the incretins glucagon-like peptide-1 and glucose-dependent insulinotropic peptide, which are efficient DPP4 substrates. FAP showed negligible or no hydrolysis of eight chemokines that are readily hydrolysed by DPP4. This novel identification of FAP substrates furthers our understanding of this unique protease by indicating potential roles in cardiac function and neurobiology. 相似文献
95.
Keane MG Dunne PG Kenny DA Berry DP 《Animal : an international journal of animal bioscience》2011,5(2):182-194
Crossbreeding of Holstein-Friesian dairy cows with both early maturing (e.g. Aberdeen Angus (AA)) and late maturing (e.g. Belgian Blue (BB)) beef breeds is commonly practised. In Ireland, genetic merit for growth rate of beef sires is expressed as expected progeny difference for carcass weight (EPD(CWT)). The objective of this study was to compare the progeny of Holstein-Friesian cows, sired by AA and BB bulls of low (L) and high (H) EPD(CWT) for performance and carcass traits. A total of 118 spring-born male progeny from 20 (9 AA and 11 BB) sires (8 L and 12 H) were managed together from shortly after birth to about 19 months of age. They were then assigned to one of two mean slaughter weights (560 kg (light) or 620 kg (heavy)). Following slaughter, carcasses were graded for conformation class and fat class, the 6th to 10th ribs joint was dissected as an indicator of carcass composition, and samples of subcutaneous fat and musculus longissimus were subjected to Hunterlab colour measurements. A sample of m. longissimus was also chemically analysed. Slaughter and carcass weights per day of age for AAL, AAH, BBL and BBH were 747, 789, 790 and 805 (s.e. 10.5) g, and 385, 411, 427 and 443 (s.e. 4.4) g, respectively. Corresponding carcass weight, kill-out proportion, carcass conformation class (scale 1 to 5) and carcass fat class (scale 1 to 5) values were 289, 312, 320 and 333 (s.e. 4.0) kg, 516, 522, 542 and 553 (s.e. 3.5) g/kg, 2.5, 2.4, 3.0 and 3.1 (s.e. 0.10), and 3.4, 3.5, 2.9 and 2.8 (s.e. 0.11). There were few breed type × genetic merit interactions. Delaying slaughter date increased slaughter weight, carcass weight and all measures of fatness. It also reduced the proportion of carcass weight in the hind quarter and the proportions of bone and muscle in the ribs joint. None of these effects accompanied the increase in carcass weight due to higher EPD(CWT). It is concluded that BB have superior production traits to AA. Selection of sires for higher EPD(CWT) increases growth rate, kill-out proportion and carcass weight of progeny with little effect on carcass or muscle traits. The extra carcass weight due to higher EPD(CWT) is more valuable commercially than a comparable carcass weight increment from a delay in slaughter date because it comprises a higher proportion of muscle. 相似文献
96.
Jenkins RK Keane A Rakotoarivelo AR Rakotomboavonjy V Randrianandrianina FH Razafimanahaka HJ Ralaiarimalala SR Jones JP 《PloS one》2011,6(12):e27570
Understanding the patterns of wild meat consumption from tropical forests is important for designing approaches to address this major threat to biodiversity and mitigate potential pathways for transmission of emerging diseases. Bushmeat consumption has been particularly poorly studied in Madagascar, one of the world's hottest biodiversity hotspots. Studying bushmeat consumption is challenging as many species are protected and researchers must consider the incentives faced by informants. Using interviews with 1154 households in 12 communes in eastern Madagascar, as well as local monitoring data, we investigated the importance of socio-economic variables, taste preference and traditional taboos on consumption of 50 wild and domestic species. The majority of meals contain no animal protein. However, respondents consume a wide range of wild species and 95% of respondents have eaten at least one protected species (and nearly 45% have eaten more than 10). The rural/urban divide and wealth are important predictors of bushmeat consumption, but the magnitude and direction of the effect varies between species. Bushmeat species are not preferred and are considered inferior to fish and domestic animals. Taboos have provided protection to some species, particularly the Endangered Indri, but we present evidence that this taboo is rapidly eroding. By considering a variety of potential influences on consumption in a single study we have improved understanding of who is eating bushmeat and why. Evidence that bushmeat species are not generally preferred meats suggest that projects which increase the availability of domestic meat and fish may have success at reducing demand. We also suggest that enforcement of existing wildlife and firearm laws should be a priority, particularly in areas undergoing rapid social change. The issue of hunting as an important threat to biodiversity in Madagascar is only now being fully recognised. Urgent action is required to ensure that heavily hunted species are adequately protected. 相似文献
97.
Coronaviruses (CoVs) are positive-sense, single-stranded, enveloped RNA viruses that infect a variety of vertebrate hosts. The CoV nucleocapsid (N) protein contains two structurally independent RNA binding domains, designated the N-terminal domain (NTD) and the dimeric C-terminal domain (CTD), joined by a charged linker region rich in serine and arginine residues (SR-rich linker). An important goal in unraveling N function is to molecularly characterize N-protein interactions. Recent genetic evidence suggests that N interacts with nsp3a, a component of the viral replicase. Here we present the solution nuclear magnetic resonance (NMR) structure of mouse hepatitis virus (MHV) nsp3a and show, using isothermal titration calorimetry, that MHV N219, an N construct that extends into the SR-rich linker (residues 60 to 219), binds cognate nsp3a with high affinity (equilibrium association constant [Ka], [1.4 ± 0.3] × 106 M−1). In contrast, neither N197, an N construct containing only the folded NTD (residues 60 to 197), nor the CTD dimer (residues 260 to 380) binds nsp3a with detectable affinity. This indicates that the key nsp3a binding determinants localize to the SR-rich linker, a finding consistent with those of reverse genetics studies. NMR chemical shift perturbation analysis reveals that the N-terminal region of an MHV N SR-rich linker peptide (residues 198 to 230) binds to the acidic face of MHV nsp3a containing the acidic α2 helix with an affinity (expressed as Ka) of 8.1 × 103 M−1. These studies reveal that the SR-rich linker of MHV N is necessary but not sufficient to maintain this high-affinity binding to N. 相似文献
98.
Almeida CA Bronke C Roberts SG McKinnon E Keane NM Chopra A Kadie C Carlson J Haas DW Riddler SA Haubrich R Heckerman D Mallal S John M 《Journal of immunology (Baltimore, Md. : 1950)》2011,187(5):2502-2513
Strong statistical associations between polymorphisms in HIV-1 population sequences and carriage of HLA class I alleles have been widely used to identify possible sites of CD8 T cell immune selection in vivo. However, there have been few attempts to prospectively and systematically test these genetic hypotheses arising from population-based studies at a cellular, functional level. We assayed CD8 T cell epitope-specific IFN-γ responses in 290 individuals from the same cohort, which gave rise to 874 HLA-HIV associations in genetic analyses, taking into account autologous viral sequences and individual HLA genotypes. We found immunological evidence for 58% of 374 associations tested as sites of primary immune selection and identified up to 50 novel HIV-1 epitopes using this reverse-genomics approach. Many HLA-adapted epitopes elicited equivalent or higher-magnitude IFN-γ responses than did the nonadapted epitopes, particularly in Nef. At a population level, inclusion of all of the immunoreactive variant CD8 T cell epitopes in Gag, Pol, Nef, and Env suggested that HIV adaptation leads to an inflation of Nef-directed immune responses relative to other proteins. We concluded that HLA-HIV associations mark viral epitopes subject to CD8 T cell selection. These results can be used to guide functional studies of specific epitopes and escape mutations, as well as to test, train, and evaluate analytical models of viral escape and fitness. The inflation of Nef and HLA-adapted variant responses may have negative effects on natural and vaccine immunity against HIV and, therefore, has implications for diversity coverage approaches in HIV vaccine design. 相似文献
99.
Peter Z. Fulé Thomas W. Swetnam Peter M. Brown Donald A. Falk David L. Peterson Craig D. Allen Gregory H. Aplet Mike A. Battaglia Dan Binkley Calvin Farris Robert E. Keane Ellis Q. Margolis Henri Grissino‐Mayer Carol Miller Carolyn Hull Sieg Carl Skinner Scott L. Stephens Alan Taylor 《Global Ecology and Biogeography》2014,23(7):825-830
Reconstructions of dry western US forests in the late 19th century in Arizona, Colorado and Oregon based on General Land Office records were used by Williams & Baker (2012; Global Ecology and Biogeography, 21 , 1042–1052; hereafter W&B) to infer past fire regimes with substantial moderate and high‐severity burning. The authors concluded that present‐day large, high‐severity fires are not distinguishable from historical patterns. We present evidence of important errors in their study. First, the use of tree size distributions to reconstruct past fire severity and extent is not supported by empirical age–size relationships nor by studies that directly quantified disturbance history in these forests. Second, the fire severity classification of W&B is qualitatively different from most modern classification schemes, and is based on different types of data, leading to an inappropriate comparison. Third, we note that while W&B asserted ‘surprising’ heterogeneity in their reconstructions of stand density and species composition, their data are not substantially different from many previous studies which reached very different conclusions about subsequent forest and fire behaviour changes. Contrary to the conclusions of W&B, the preponderance of scientific evidence indicates that conservation of dry forest ecosystems in the western United States and their ecological, social and economic value is not consistent with a present‐day disturbance regime of large, high‐severity fires, especially under changing climate. 相似文献
100.
Oil and gas projects in the Western Amazon: threats to wilderness, biodiversity, and indigenous peoples 总被引:3,自引:0,他引:3