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排序方式: 共有557条查询结果,搜索用时 15 毫秒
81.
T. Raz Tim Barrett Raymonde Szargel Hanna Mandel Ellis J. Neufeld Kazuto Nosaka Marcos B. Viana N. Cohen 《Human genetics》1998,103(4):455-461
Thiamine-responsive megaloblastic anemia (TRMA, also known as Rogers syndrome, OMIM 249270) is a rare autosomal recessive
disorder characterized by a triad of megaloblastic anemia, diabetes mellitus, and sensorineural deafness. Patients respond,
to varying degrees, to treatment with megadoses of thiamine. We have recently shown genetic linkage of the TRMA gene to a
16-centimorgan (cM) region on 1q23.2–1q23.3 based on the analysis of four large, inbred families of Alaskan, Italian, and
Israeli-Arab origin. Here we narrow the TRMA interval down to 4 cM based on genetic recombination, homozygosity mapping, and
linkage disequilibrium (highest LOD score of 12.5 at D1S2799, at a recombination fraction of 0). We provide further evidence that the TRMA gene is located in this region and confirm
the homogeneity of the disease. In this analysis, we genotyped seven additional families of diverse ethnic origin (Pakistani,
Indian, Italian, Brazilian, and Japanese), and analyzed additional markers in two previously reported families showing evidence
of linkage disequilibrium in a large area of their haplotypes. The multi-system manifestations of TRMA suggest that thiamine
has a pivotal role in a multiplicity of physiological processes. Mapping the TRMA gene and understanding the molecular basis
of the disease might, thus, shed light on the role of thiamine in common disorders such as deafness, anemia, and diabetes.
Received: 16 April 1998 / Accepted: 6 July 1998 相似文献
82.
N Morisaki S Watanabe K Fukuda Y Saito 《Cellular and molecular biology, including cyto-enzymology》1999,45(1):67-77
Retinal endothelial cells (ECs) and pericytes (PCs) were cloned and cultured from normal and diabetic rabbits to clarify the mechanism of diabetic proliferative retinopathy from the viewpoint of the interaction between ECs and PCs, and phenotypic changes of diabetic cells. PC-conditioned medium (PC-CM) from normal rabbits stimulated in vitro angiogenesis of diabetic ECs more than that of normal ECs. in vitro angiogenesis was also more stimulated in diabetic ECs than in normal ECs by basic fibroblast growth factor (bFGF) or transforming growth factor-beta 1, indicating that diabetic ECs are different from normal ECs in terms of angiogenic potential. One mechanism of this property of diabetic ECs was the acceleration of cell proliferation but not of cell migration, because diabetic ECs grew more rapidly but did not migrate more than normal ECs in response to PC-CM or bFGF. Moreover, PC-CM from diabetic PCs stimulated angiogenesis of normal ECs more than that from normal PCs, indicating that diabetic PCs secreted more angiogenic factor(s) than normal PCs. The angiogenic, mitogenic and migratory activities of PC-CM both from normal and diabetic PCs were similarly inhibited by an anti-bFGF antibody. Western blot analysis revealed this factor to be a bFGF-like molecule. These data indicate that the interaction between ECs and PCs and the phenotypic changes of diabetic ECs and PCs both contribute to the proliferative retinopathy in diabetes. 相似文献
83.
Y Nishiyama T Hirota T Morisaki T Hara T Marumoto S Iida K Makino H Yamamoto T Hiraoka N Kitamura H Saya 《FEBS letters》1999,459(2):159-165
We identified a human homolog of Drosophila warts tumor suppressor gene, termed h-warts, which was mapped at chromosome 6q24-25.1. The h-warts protein has a serine/threonine kinase domain and is localized to centrosomes in interphase cells. However, it becomes localized to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, in a highly dynamic manner during mitosis. Furthermore, h-warts is specifically phosphorylated in cells at mitotic phase, most likely by Cdc2 kinase. These findings suggest that h-warts functions as a component of the mitotic apparatus and is involved in proper progression of mitosis. 相似文献
84.
Tanaka Y Ogasawara T Asawa Y Yamaoka H Nishizawa S Mori Y Takato T Hoshi K 《Cell biology international》2008,32(5):505-514
To discuss the autologous serum production for cartilage tissue engineering, we compared three kinds of sera: whole blood-derived serum (WBS), platelet-containing plasma-derived serum (PCS), and plasma-derived serum (PDS), on the growth factor contents and their biological effects on human auricular chondrocytes. EGF, VEGF and PDGF levels were highest in WBS, while PCS and PDS followed WBS. The proliferation effects of WBS were the most pronounced, followed by that of PCS, both of which realized a 1000-fold-increase in chondrocyte numbers at the third passage, whereas PDS reached it after passage 4. No significant differences were observed in histology or cartilaginous matrix measurements of tissue-engineered cartilage produced from chondrocytes cultured under different serum conditions. WBS would be clinically useful because of its potent proliferation effects, while PCS, which possibly saves the red cell concentrate, may be an option in cases where there are elevated risks of blood loss. 相似文献
85.
Liu D Yumoto H Hirota K Murakami K Takahashi K Hirao K Matsuo T Ohkura K Nagamune H Miyake Y 《Cellular microbiology》2008,10(1):262-276
Streptococcus intermedius is a commensal associated with serious, deep-seated purulent infections in major organs, such as the brain and liver. Histone-like DNA binding protein (HLP) is an accessory architectural protein in a variety of bacterial cellular processes. In this study, we investigated the mechanisms of pro-inflammatory cytokine inductions in THP-1 cells by stimulation with recombinant HLP of S. intermedius (r Si -HLP). r Si -HLP stimulation-induced production of pro-inflammatory cytokines (IL-8, IL-1β and TNF-α) occurred in a time- and dose-dependent manner. In contrast with the heat-stable activity of DNA binding, the induction activity of r Si -HLP was heat-unstable. In subsequent studies, r Si -HLP acted cooperatively with lipoteichoic acid, the synthetic Toll-like receptor 2 agonist, Pam3CSK4, and the cytosolic nucleotide binding oligomerization domain 2 receptor agonist, muramyldipeptide. Furthermore, Western blot and blocking assays with specific inhibitors showed that r Si -HLP stimulation induced the activation of cell signal transduction pathways, extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase (JNK). In addition to its physiological role in bacterial growth through DNA binding, these results indicate that Si -HLP can trigger a cascade of events that induce pro-inflammatory responses via ERK1/2 and JNK signal pathways, and suggest that bacterial HLP may contribute to the activation of host innate immunity during bacterial infection. 相似文献
86.
87.
88.
Ko Nifuku Kazuto Kodama Yasunari Shigeta Hajime Naruse 《Palaeogeography, Palaeoclimatology, Palaeoecology》2009,271(1-2):84-95
A combined magnetostratigraphic and biostratigraphic study has been performed on the Maastrichtian Senpohshi Formation in eastern Hokkaido Island, northern Japan, which is an approximately 1300 m thick section mainly composed of hemipelagic mudstone. The identification of magnetic polarity was possible at 51 horizons, whereby four magnetozones were recognized. These magnetozones were correlatable to geomagnetic polarity chrons C31r to C30n, suggesting that the age of the Senpohshi Formation is spanning from middle to upper part of the Maastrichtian (ca. 69–67 Ma).The magnetostratigraphy of the Senpohshi Formation established in this study enables a direct age correlation to the Maastrichtian successions in other regions. Thus, this detailed chronology of the formation contributes to paleontological studies of the Maastrichtian in the North Pacific region. For instance, this magnetostratigraphic age assessment implies the following: (1) the stratigraphic range of the ammonite Pachydiscus flexuosus contains polarity chrons from the lower part of C31r to the lower part of C31n, (2) the first occurrence (FO) of the calcareous nannofossil Nephrolithus frequens in the North Pacific region is correlatable to polarity chron C30n or below, and (3) the FO of the bivalve “Inoceramus” awajiensis is located within polarity chrons from C31r to the upper part of C31n. This suggests that the inoceramid extinction event in the North Pacific region might have occurred during polarity chrons from C31r to the upper part of C31n (ca. 70.5–67.8 Ma), which is 2.3–5.0 Myr prior to the Cretaceous/Paleogene boundary. The trend of the Maastrichtian faunal turnover in the North Pacific is well consistent with those of other regions, brings a new evidence for understanding the global faunal turnover in the Maastrichtian, just before Cretaceous/Paleogene mass extinction. 相似文献
89.
Yoshinori Harada Ping Dai Yoshihisa Yamaoka Mitsugu Ogawa Hideo Tanaka Kazuto Nosaka Kenichi Akaji Tetsuro Takamatsu 《Histochemistry and cell biology》2009,132(1):39-46
Most molecular imaging technologies require exogenous probes and may have some influence on the intracellular dynamics of
target molecules. In contrast, Raman scattering light measurement can identify biomolecules in their innate state without
application of staining methods. Our aim was to analyze intracellular dynamics of topoisomerase I inhibitor, CPT-11, by using
slit-scanning confocal Raman microscopy, which can take Raman images with high temporal and spatial resolution. We could acquire
images of the intracellular distribution of CPT-11 and its metabolite SN-38 within several minutes without use of any exogenous
tags. Change of subcellular drug localization after treatment could be assessed by Raman imaging. We also showed intracellular
conversion from CPT-11 to SN-38 using Raman spectra. The study shows the feasibility of using slit-scanning confocal Raman
microscopy for the non-labeling evaluation of the intracellular dynamics of CPT-11 with high temporal and spatial resolution.
We conclude that Raman spectromicroscopic imaging is useful for pharmacokinetic studies of anticancer drugs in living cells.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
90.
Shinichi Kuriyama Yasushi Taguchi Kazuto Watanabe Kanako Nishimura Kazutoshi Yanagibashi Yoshiki Katayama Takuro Niidome 《Bioorganic & medicinal chemistry》2009,17(22):7643-7646
The amphiphilic α-helical peptide, Td3717, is a bi-functional synthetic peptide that acts as both a polycation for DNA binding and a ligand for targeted delivery to tumor cells. Td3717 forms a stable complex with plasmid DNA, and the complex maintained high transfection efficiency after storage at 4 °C for six months and after four freeze/thaw cycles. During the storage and freeze/thaw cycling, the particle size of the DNA/Td3717 complex remained less than 100 nm. The size of the complex is an important factor for its internalization into cells via the endocytosis pathway; therefore, the stability of the particles will strongly contribute to high transfection efficiencies after storage and repeated freezing/thawing. 相似文献