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71.
Effect of host shoot clipping on carbon and nitrogen sources for arbuscular mycorrhizal fungi 总被引:2,自引:0,他引:2
The effect of clipping of the host-plant shoot on the sources of carbon and nitrogen for the arbuscular mycorrhizal (AM) fungus Gigaspora margarita was determined by measuring 13C in spores and hyphae in cocultures of C3 and C4 plants and by differential 15N labeling. C3 and C4 plants, which have different δ13C values, were grown in the same container separated by a series of hyphal compartments. The C3 and C4 plants were applied with 14N- and 15N-urea, respectively. After clipping of the C3 shoots, spore δ
13C gradually approached that of the C4 roots. Hyphal δ
13C paralleled that of spores. Spore % 15N was similar to that of mineral N in the C4 plant compartment. Thus C in G. margarita coming from the clipped plants decreased with time. This demonstrates that C in AM fungi comes from living plants, whilst
the N in spores comes mostly from the soil.
Accepted: 28 November 2000 相似文献
72.
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74.
Haruna Ishikawa Xiaoxu Guo Saeko Sugawara Yui Iwagaki Kazushi Yamamoto 《Bioscience, biotechnology, and biochemistry》2018,82(3):515-524
We examined the effects on offspring of ingestion of the 1975 Japanese diet during pregnancy and lactation and after weaning in mice. Pregnant dams were divided into groups that were fed the Japanese diet or a control diet and raised until offspring were weaned. The offspring after weaning were further divided into groups that were raised on the Japanese diet or the control diet. Ingestion of the Japanese diet after weaning suppressed accumulation of visceral fat in offspring, and reduced the amount of lipids in serum and liver. This effect was weakened if the Japanese diet was only ingested during pregnancy and lactation. Therefore, it was suggested that ingestion of the Japanese diet of mothers during pregnancy and lactation weakens the lipid accumulation inhibitory effect of the Japanese diet in children. 相似文献
75.
Kazushi Yukiiri Naohisa Hosomi Takayuki Naya Tsutomu Takahashi Hiroyuki Ohkita Mao Mukai Hisashi Masugata Koji Murao Masaki Ueno Takehiro Nakamura Hiroaki Dobashi Takanori Miki Yasuhiro Kuroda Masakazu Kohno 《BMC neurology》2008,8(1):1-8
Background
Cardioembolic stroke generally results in more severe disability, since it typically has a larger ischemic area than the other types of ischemic stroke. However, it is difficult to differentiate cardioembolic stroke from non-cardioembolic stroke (atherothrombotic stroke and lacunar stroke). In this study, we evaluated the levels of plasma brain natriuretic peptide in acute ischemic stroke patients with cardioembolic stroke or non-cardioembolic stroke, and assessed the prediction factors of plasma brain natriuretic peptide and whether we could differentiate between stroke subtypes on the basis of plasma brain natriuretic peptide concentrations in addition to patient's clinical variables.Methods
Our patient cohort consisted of 131 consecutive patients with acute cerebral infarction who were admitted to Kagawa University School of Medicine Hospital from January 1, 2005 to December 31, 2007. The mean age of patients (43 females, 88 males) was 69.6 ± 10.1 years. Sixty-two patients had cardioembolic stroke; the remaining 69 patients had non-cardioembolic stroke (including atherothrombotic stroke, lacunar stroke, or the other). Clinical variables and the plasma brain natriuretic peptide were evaluated in all patients.Results
Plasma brain natriuretic peptide was linearly associated with atrial fibrillation, heart failure, chronic renal failure, and left atrial diameter, independently (F4,126 = 27.6, p < 0.0001; adjusted R2 = 0.45). Furthermore, atrial fibrillation, mitral regurgitation, plasma brain natriuretic peptide (> 77 pg/ml), and left atrial diameter (> 36 mm) were statistically significant independent predictors of cardioembolic stroke in the multivariable setting (Χ2 = 127.5, p < 0.001).Conclusion
It was suggested that cardioembolic stroke was strongly predicted with atrial fibrillation and plasma brain natriuretic peptide. Plasma brain natriuretic peptide can be a surrogate marker for cardioembolic stroke. 相似文献76.
77.
Shin Iwamoto Norihisa Nishimichi Yoshiko Tateishi Yuko Sato Hiroyuki Horiuchi Shuichi Furusawa Tatsuya Sawamura Haruo Matsuda 《MABS-AUSTIN》2009,1(4):357-363
Lectin-like oxidized low-density lipoprotein (LDL) receptor-1 (LOX-1) is the major receptor for oxidized LDL (oxLDL), and plays a key role in the pathogenesis of atherosclerosis and cardiovascular diseases. Monoclonal antibodies (mAbs) specific for human LOX-1 (hLOX-1) were generated by a phage display technique using chickens immunized with recombinant hLOX-1 (rhLOX-1). A total of 53 independent scFv clones reactive for rhLOX-1 were obtained. Of the 53 clones, 49 recognized the C-type lectin-like domain (CTL domain), which contributes to the binding of oxLDL. Of these, 45 clones inhibited oxLDL-binding with LOX-1. Furthermore, some of these clones cross-reacted with rabbit, pig and/or mouse LOX-1. For possible application as therapeutic agents in the future, two cross-reactive mAbs were re-constructed as chicken-human chimeric antibodies. The chimeric antibodies showed similar characteristics compared to the original antibodies, and inhibited oxLDL binding to LOX-1 expressed on CHO cells. The results obtained in this study indicate that anti-LOX-1 mAbs might be useful tools for functional analyses and development of therapeutic agents for cardiovascular indications such as atherosclerosis.Key words: LOX-1, oxLDL, chicken monoclonal antibody, chimeric antibody, neutralizing antibody 相似文献
78.
Jun Sawai Kazushi Matsumoto Taka-aki Saito Yasuhiko Isomura Risei Wada 《International biodeterioration & biodegradation》2009,63(2):196-200
The influence of infrared (IR) radiation on the viability and heat-activation of Bacillus subtilis spores, suspended in phosphate-buffered saline, was investigated. Two types of IR heaters with different spectral distributions were used. Near-infrared (NIR) and far-infrared (FIR) heaters with main wavelengths of approximately 1 μm and 3–6 μm, respectively, were utilized. Although both irradiation treatments decreased the number of B. subtilis colonies at a bulk temperature of approximately 75 °C, the mode of action was clearly different. In the case of the NIR heater, the number of colony-counts decreased gradually. In contrast, use of the FIR heater resulted in heat activation of the spores during the early stage of irradiation at a low bulk temperature (40–60 °C) over several minutes, followed by a decrease in the number of colonies. Consequently, FIR irradiation inactivated 90% of B. subtilis spores more effectively as compared to NIR irradiation for 20 min with a suspension volume of 20 ml and irradiation energy of 7.57 kW m?2. Spore exposure to FIR irradiation accelerated their germination rate in nutrient broth; however, this was not true for treatment with the NIR heater. The absorption IR spectrum of B. subtilis spores indicated that FIR radiation was absorbed easily by the spore cell components and might activate the bioactive substances involved in germination. Even at the same irradiation energy, the influence of infrared radiation on spore germination was dependent on the IR spectral distribution. Bacterial spores undergoing germination lose their resistance to stressors, such as heat, chemicals and ultraviolet rays. FIR heating promotes heat activation and germination, thereby producing vegetative cells that are more susceptible to other killing methods, enabling the killing of bacterial spores at lower stress without product damage. 相似文献
79.
Makoto Yanagihara Masako Kawasaki Hiroshi Ishizaki Kazushi Anzawa Shun-ichi Udagawa Takashi Mochizuki Yukio Sato Nobuyuki Tachikawa Hiroyoshi Hanakawa 《Mycoscience》2010,51(3):224-233
A 74-year-old healthy Japanese man had noticed an itchy lesion with two tiny thick brown scales on the fourth interdigital
web of his right foot 3 months earlier. The brown fungus isolated from the scales had demonstrated brown, ellipsoidal, obovoid
or clavate, 3-septate conidia. Morphologically, the fungus was identified as Curvularia sp., and was differentiated from other human pathogenic species of the genus, such as C. lunata, C. pallescens, C. trifolii, C. clavata, and C. brachyspora, by molecular analysis based on the DNA sequence data. The fungus grows well on keratotic materials (hairs, nails, and callus),
which indicates that it might have the ability to infect the skin surface. 相似文献
80.
Kentaro Honda Nobuyuki Takahashi Keiichi Yamamoto Haruka Kagiwada Yuichi Tsuda Yoko Mitani Kazushi Miyashita 《Ichthyological Research》2017,64(3):357-364
Behavior of adult Parahucho perryi was examined using bio-logging and acoustic telemetry concurrently in the Bekanbeushi River system, eastern Hokkaido, Japan, in 2009 and 2010. Based on 46.1–87.9 h data from five P. perryi (69.0–80.0 cm fork length) caught from Lake Akkeshi, they used upstream (n = 2), midstream (n = 3), and downstream (n = 4) habitats. Large variability in diel activity and depth occupation existed in each stream habitat; however, fish in the downstream habitat tended to be more active than those in the upper habitats and mainly occupied shallower depths than mean bottom depth in this habitat. 相似文献