全文获取类型
收费全文 | 4519篇 |
免费 | 233篇 |
国内免费 | 1篇 |
专业分类
4753篇 |
出版年
2022年 | 15篇 |
2021年 | 29篇 |
2019年 | 21篇 |
2018年 | 34篇 |
2017年 | 36篇 |
2016年 | 59篇 |
2015年 | 97篇 |
2014年 | 119篇 |
2013年 | 483篇 |
2012年 | 223篇 |
2011年 | 230篇 |
2010年 | 157篇 |
2009年 | 154篇 |
2008年 | 232篇 |
2007年 | 269篇 |
2006年 | 236篇 |
2005年 | 270篇 |
2004年 | 307篇 |
2003年 | 244篇 |
2002年 | 254篇 |
2001年 | 45篇 |
2000年 | 38篇 |
1999年 | 50篇 |
1998年 | 56篇 |
1997年 | 55篇 |
1996年 | 39篇 |
1995年 | 49篇 |
1994年 | 49篇 |
1993年 | 54篇 |
1992年 | 53篇 |
1991年 | 49篇 |
1990年 | 37篇 |
1989年 | 38篇 |
1988年 | 38篇 |
1987年 | 39篇 |
1986年 | 43篇 |
1985年 | 44篇 |
1984年 | 51篇 |
1983年 | 46篇 |
1982年 | 48篇 |
1981年 | 58篇 |
1980年 | 47篇 |
1979年 | 33篇 |
1978年 | 32篇 |
1977年 | 23篇 |
1976年 | 35篇 |
1975年 | 19篇 |
1974年 | 22篇 |
1973年 | 20篇 |
1972年 | 14篇 |
排序方式: 共有4753条查询结果,搜索用时 15 毫秒
141.
Miyamoto T Hasuike S Sengoku K Takuma N Hayashi H Sasaki Y Yamashita T Ishikawa M 《Development genes and evolution》2003,213(4):199-202
Mouse Spot-1 is a DNA-binding protein with a domain (His-Thr) encoded by p(CA)n repeats. Spot-1 interacts with the nuclear localization signal (NLS) I of p53 through its His-Thr domain. In this study we describe the cloning and expression patterns of a novel gene encoding a protein containing a His-Thr domain, Spot-2. Spot-2 is exclusively expressed in the pituitary from stage E13.5 to E15.5. Mouse Lhx3 plays a critical role during early organogenesis in the pituitary. The Spot-2 gene appears to be a downstream gene of Lhx3. It is suggested that Spot-2 plays important roles in pituitary development. 相似文献
142.
Suzuki F Hayakawa M Nakagawa T Nasir UM Ebihara A Iwasawa A Ishida Y Nakamura Y Murakami K 《The Journal of biological chemistry》2003,278(25):22217-22222
We investigated the mechanism for non-proteolytic activation of human prorenin using five kinds of antibodies. Each of the antigens, L1PPTDTTTFKRI11P, T7PFKRIFLKRMP17P, I11PFLKRMPSIRESLKER26P, M16PPSIRESLKER26P, and G27PVDMARLGPEWSQPM41P, was designed from the tertiary structure of predicted prorenin. These antibodies were labeled anti-01/06, anti-07/10, anti-11/26, anti-16/26, and anti-27/41, respectively, for their binding specificities. Inactive recombinant human prorenin (0.1 nM) bound to various concentrations of anti-01/06, anti-11/26, and anti-27/41 antibodies at 4 degrees C with equilibrium dissociation constants of 138, 41, and 22 nM, respectively. However, intact prorenin (0.1 nM) did not show significant binding to 200 nM anti-07/10 and anti-16/26 antibodies for 20 h. Ninety percent of prorenin (0.1 nM) was found to be non-proteolytically activated by incubation with anti-11/26 antibodies (200 nM) at 4 degrees C for 20 h. Prorenin was not active even under complex with either anti-01/06 or anti-27/41 antibodies. Prorenin was also reversibly activated at pH 3.3 and 4 degrees C for 25 h. The acid-activated prorenin bound to anti-07/10 and anti-16/26 antibodies as well as to anti-01/06, anti-11/15, and anti-27/41 antibodies at neutral pH and 4 degrees C in 2 h. Their dissociation constants were 13, 40, 8.6, 3.6, and 14 nM, respectively. The acid-activated prorenin was re-inactivated by incubation at pH 7.4 and 4 degrees C in 50 h. Anti-07/10 and anti-11/26 antibodies inhibited such re-inactivation at 25 degrees C by more than 90% and 50%, respectively, whereas other kinds of antibodies did not prevent the re-inactivation at 25 degrees C. These results indicate that prorenin has "gate" (T7PFKR10P) and "handle" (I11PFLKR15P) regions critical for its non-proteolytic activation. 相似文献
143.
144.
Uchida H Kondo D Yamashita A Nagaosa Y Sakurai T Fujii Y Fujishiro K Aisaka K Uwajima T 《FEMS microbiology letters》2003,229(1):31-36
An aldehyde oxidase, which oxidizes various aliphatic and aromatic aldehydes using O(2) as an electron acceptor, was purified from the cell-free extracts of Pseudomonas sp. KY 4690, a soil isolate, to an electrophoretically homogeneous state. The purified enzyme had a molecular mass of 132 kDa and consisted of three non-identical subunits with molecular masses of 88, 39, and 18 kDa. The absorption spectrum of the purified enzyme showed characteristics of an enzyme belonging to the xanthine oxidase family. The enzyme contained 0.89 mol of flavin adenine dinucleotide, 1.0 mol of molybdenum, 3.6 mol of acid-labile sulfur, and 0.90 mol of 5'-CMP per mol of enzyme protein, on the basis of its molecular mass of 145 kDa. Molecular oxygen served as the sole electron acceptor. These results suggest that aldehyde oxidase from Pseudomonas sp. KY 4690 is a new member of the xanthine oxidase family and might contain 1 mol of molybdenum-molybdpterin-cytosine dinucleotide, 1 mol of flavin adenine dinucleotide, and 2 mol of [2Fe-2S] clusters per mol of enzyme protein. The enzyme showed high reaction rates toward various aliphatic and aromatic aldehydes and high thermostability. 相似文献
145.
146.
Discoidin domain receptor 2 interacts with Src and Shc following its activation by type I collagen 总被引:5,自引:0,他引:5
Ikeda K Wang LH Torres R Zhao H Olaso E Eng FJ Labrador P Klein R Lovett D Yancopoulos GD Friedman SL Lin HC 《The Journal of biological chemistry》2002,277(21):19206-19212
Discoidin domain receptor 2 (DDR2) is an unusual receptor tyrosine kinase in that its ligand is fibrillar collagen rather than a growth factor-like peptide. We examined signal transduction pathways of DDR2. Here we show that DDR2 is also unusual in that it requires Src activity to be maximally tyrosine-phosphorylated, and that Src activity also promotes association of DDR2 with Shc. The interaction with Shc involves a portion of Shc not previously implicated in interaction with receptor tyrosine kinases. These results identify Src kinase and the adaptor protein Shc as key signaling intermediates in DDR2 signal transduction. Furthermore, Src is required for DDR2-mediated transactivation of the matrix metalloproteinase-2 promoter. The data support a model in which Src and the DDR2 receptor cooperate in a regulated fashion to direct the phosphorylation of both the receptor and its targets. 相似文献
147.
148.
Thalidomide and its analogues as cyclooxygenase inhibitors 总被引:6,自引:0,他引:6
Noguchi T Shimazawa R Nagasawa K Hashimoto Y 《Bioorganic & medicinal chemistry letters》2002,12(7):1043-1046
Thalidomide showed cyclooxygenase (COX)-1/2 inhibitory activity with a potency comparable to that of aspirin. Structural development studies of thalidomide resulted in potent COX-1/2 inhibitors, and COX-1-selective and COX-2-selective inhibitors. 相似文献
149.
150.
Gibberellic-acid (GA3) treatment of azukibean epicotyls resulted in alterations of the direction of newly deposited microfibrils, on the cell walls. Cells having transverse microfibrils on the inner surface of the wall were observed more frequently in GA3-treated epicotyls than in untreated or water-treated ones. This effect of GA3 was negated by simultaneously supplied colchicine. A crossed polylamellate structure was observed in the inner portion of the walls of GA3-treated cells, but not in the inner portion of the walls of colchicine-treated cells. The wall formed under the influence of colchicine consisted of microfibrils running in the same direction.Abbreviations GA
gibberellin
- GA3
gibberellic acid (gibberellin A3) 相似文献