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991.
Kazuo Sato Toyokuni Honma Soji Sugai 《Bioscience, biotechnology, and biochemistry》2013,77(12):3563-3567
Many 1,2-benzisoxazole-3-acetamides were synthesized and their herbicidal activities in the paddy field were studied. Of the compounds tested, N-α,α-dimethylbenzyl-2-bromo-(1,2-benzisoxazol-3-yl)acetamide 10a was the most effective. Details of the synthesis and the results of herbicidal evaluations are given. 相似文献
992.
A protease from the lotus seed (Nelumbo nucifera Gaertn) was purified by acid-treatment, ammonium sulfate-fractionation, ethylalcohol-fractionation, TEAE-cellulose-treatment and Sephadex G-100 gel-filtration.The enzyme was purified about 870-fold and was homogeneous in electrophoretic and ultracentrifugal analyses.Purified lotus seed protease is an acid protease with a pH optimum at 3.8 toward urea-denatured casein. It is active for casein and hemoglobin. But other proteins such as edestin, zein, lotus seed globulin and soybean casein are slightly hydrolyzed and egg albumin is hardly hydrolyzed. This enzyme is most stable at pH 4.0 below 40°C. The enzyme is not a thiol protease, and its activity was completely inhibited by potassium permanganate, remarkably inhibited by sodium dodecylsulfate and accelerated by hydrogen peroxide. 相似文献
993.
994.
Kazuo Isozumi Robert DeLong Jocelyn Kaplan Wu-Yen Hung T. Siddique 《Human genetics》1997,99(6):701-703
Scapuloperoneal spinal muscular atrophy (SPSMA) is a neuromuscular disorder characterized by weakness in the distribution
of shoulder girdle and peroneal muscles. We have previously described a large New England kindred with autosomal dominant
SPSMA and have subsequently linked this family trait to 12q24.1-q24.31. In this family, disease expression becomes more severe
and progressive in successive generations, suggesting genetic anticipation. Accordingly, we have investigated the thirteen
known CAG/CTG repeat loci on chromosome 12 that could be tested by using the polymerase chain reaction as candidate genetic
mutations in SPSMA. None of these loci is expanded.
Received: 12 June 1996 / Revised: 24 January 1997 相似文献
995.
Identification of the replication region of Lactobacillus acidophilus plasmid pLA103 总被引:1,自引:0,他引:1
Kazuo Kanatani Takatsugu Tahara Masao Oshimura Keiji Sano chisae Umezawa 《FEMS microbiology letters》1995,133(1-2):127-130
Abstract The structure of the region necessary for replication of the plasmid pLA103 from Lactobacillus acidophilus TK8912 has been characterized. Sequence analysis revealed that the replication region contained an open reading frame (OrfA) encoding a 282-amino acid peptide preceded by a 22-bp tandem repeat sequence region. The predicted OrfA protein showed homology to the replication protein of a plasmid from Pediococcus halophilus . The plasmid containing the repeat sequence region preceding OrfA was able to replicate in the Lactobacillus host when provided with OrfA in trans , suggesting that the repeat sequence region contains the origin sequence essential for the pLA103 replication. 相似文献
996.
997.
998.
Fumiyasu Fukumori Kazuo Ohishi Toshiaki Kudo Koki Horikoshi 《FEMS microbiology letters》1987,48(1-2):65-68
Abstract A 5.7-kb Eco RI DNA fragment has been isolated from Bacillus sp. strain N-4 chromosome DNA. This fragment contained both the pNK1-encoded cellulase ( celB ) gene and the pNK2-encoded cellulase ( celA ) gene which were highly homologous [13]. These results demonstrate the tandem location of these genes on the chromosomal DNA. The homologous sequence, which may play an important role for the gene duplication, were observed 5' upstream of the celA gene, between the celA and celB genes, and 3' downstream from the celB gene. 相似文献
999.
1000.
Etsuko Kobayashi Naoko Sugihira Kazuo T. Suzuki 《Biological trace element research》1991,28(3):187-194
Effects of aging on the biological discrimination between calcium (Ca) and strontium (Sr) by the kidneys and bone were studied in male and female rats of 5 to 50 wk of age by examining Sr/Ca ratios in the plasma, urine, and bone. The Ca-Sr discrimination at the reabsorption process in the kidneys was not affected by aging in male or female rats. On the other hand, discrimination between the two elements was shown to be age-related at the absorption process in the digestive tract, and became more strict with age. The reverse situation was observed in the discrimination of Ca and Sr in the femur; younger rats discriminated the two elements more strictly than older animals. 相似文献