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951.
A porometer, which can be easily constructed with a common photometricunit and yet records the air flow through a leaf blade accuratelyand sensitively, was developed and used to record the flow changesthrough Brassica chinensis leaves under several light, air andwater conditions. (Received May 17, 1973; ) 相似文献
952.
Kazuo Yamashita 《Cell and tissue research》1972,124(4):465-478
Summary Anterior pituitaries of mice were incubated for periods up to four hours in Krebs-Ringer bicarbonate glucose gassed with 95% O25% CO2. The incubated explants survived and retained a fine structure that approximated the condition in situ. The few necrotic cells were sharply localized, and were found to be due to initial mechanical damage to the tissue.Some cells of the six granulated types exhibited slight but significant changes attributable to the liberation from the hypothalamic control: in LTH cells there was a release of preexisting granules and a development of cell organelles, whereas in other cell types there was an inhibition of release of granules and an enhanced digestion of the accumulated granules by the lysosomal system.Follicular cells responded uniquely to the changed environment by hypertrophy of the cytoplasm and were found to phagocytize cell debris. A part of non-epithelial elements of the gland showed a tendency to modulate cytologically.The author would like to express his appreciation to Mr. T. Anzai and Mr. S. Terada for their excellent technical assistance. 相似文献
953.
Isoprenoid quinones and fatty acids ofZoogloea 总被引:1,自引:0,他引:1
Akira Hiraishi Yong Kook Shin Junta Sugiyama Kazuo Komagata 《Antonie van Leeuwenhoek》1992,61(3):231-236
NineZoogloea strains including the type strain ofZ. ramigera (IAM 12136=ATCC 19544=N.C. Dondero 106) and newly isolated strains were investigated for isoprenoid quinone composition and whole-cell fatty acid profiles. Seven of the tested strains, having phenotypic properties typical ofZoogloea, were characterized by their production of both ubiquinone-8 and rhodoquinone-8 as major quinones, whereas the remaining two strains,Z. ramigera IAM 12669 (=K. Crabtree I-16-M) and IAM 12670 (=P.R. Dugan 115), formed ubiquinone-10 and ubiquinone-8, respectively, as the sole quinone. All rhodoquinone-producing strains contained palmitoleic acid and 3-hydroxy-decanoic acid as the major components of nonpolar and hydroxylated fatty acids, respectively. Marked differences were noted in the fatty acid composition between the strains with and without rhodoquinones. The chemotaxonomic data suggested that the rhodoquinonelacking strains should be excluded from the genusZoogloea. Since there have been no reliable taxonomic tools forZoogloea, rhodoquinone analysis may provide a new criterion of great promise for identifyingZoogloea strains. 相似文献
954.
From Picrasma javanica, five new quassinoid glucosides, javanicinosides D-H, together with known quassinoids, neoquassin and picrasin A and triterpenoids, hispidol A and lanosta-7,24-dien-3-one were isolated. The structures have been determined by spectral analysis and chemical evidence. 相似文献
955.
Shinobu Tanaka Takakazu Suzuki Mitsuru Sakaizumi Yoshinobu Harada Yoshibumi Matsushima Nobumoto Miyashita Yasuo Fukumori Shinya Inai Kazuo Moriwaki Hiromichi Yonekawa 《Immunogenetics》1991,33(1):18-23
Sera from 73 strains of mice were tested for hemolytic activity through the classical and the alternative pathways (CP and AP) in a single radical hemolysis assay. Sera from 16 out of 45 laboratory inbred strains had n o lytic activity, and Ouchternoly analysis with anti-C5 serum showed them to be C5-deficient. Sera from 2 out of 28 strains derived from wild mice also had no lytic activity, but the C5 molecule was detectable in both. The hemolytic activity of sera from these strains can be restored serum deficient in C8-, leading us to conclude that strains M.MOL-MSM (MSM) and Mae are deficient in the subunit of C8. Typing of (DBA/2J ×MSM)F1 hybrids and of progeny of a backcross to MSM showed that this C8 deficiency is controlled by a singles recessive gene, designated C8b; the allele with hemolytic activity is C8b
1; and the allele with no activity C8b
0. Because of synteny homologies in mouse and human , we looked for and found close linkage between C8b and Pgm-2. Typing of recombinant mice for Mup-1 mapped the C8b locus 2.3 centimorgans (cM) telomeric to Pgm-2 on mouse chromosome 4. 相似文献
956.
957.
Kazuo Shinozaki Chong-Rong Sun Masahiro Sugiura 《Molecular & general genetics : MGG》1984,197(3):363-367
Summary Six protein genes have been mapped on broad bean chloroplast DNA by Southern hybridization using the tobacco chloroplast genes as probes. In broad bean chloroplast DNA, the genes for the and subunits of proton-translocating ATPase and the 32,000 dalton thylakoid membrane protein are located near the large subunit gene of ribulose-1,5-bisphosphate carboxylase/oxygenase. The gene for the subunit of proton-translocating ATPase is distantly located from the and genes. The gene for the ribosomal protein CS19 was found close to the ribosomal RNA operon. The gene organization of broad bean chloroplast DNA is therefore quite different from that of tobacco chloroplast DNA. The nucleotide sequence of the spacer region between the large subunit and the genes of the broad bean has been determined. Conserved sequences are found among the putative promoter regions of the chloroplast protein genes. 相似文献
958.
The Protein Journal - The amino acid sequences of the tetracycline-resistance (Tcr) determinants of Bacillus subtilis plasmid pNS1981 and Staphylococcus aureus plasmid pTP5 have been deduced from... 相似文献
959.
This study was performed in order to identify the fungi of four species (Aspergillus fumigatus, Fusarium anthophilum, Candida albicans, Cryptococcus neoformans) in formalin-fixed, paraffin-embedded tissue sections by the indirect method of immunoperoxidase staining. Mature albino rabbits were immunized by formalin-killed organisms. The antibodies were prepared by precipitation at a 50% saturation of ammonium sulfate and were checked for cross-reactivities by Ouchterlony's double immunodiffusion and precipitin test. The immunoperoxidase staining was applied to the paraffin-embedded tissue sections of infected mice, human autopsy and biopsy specimens. Although each fungus was stained clearly the cell wall, cross-reactivities appeared among them, however it was possible to identify four fungi by absorption and dilution of the antisera. 相似文献
960.
Hiroyuki Tsuda Tadashi Inoue Makoto Asamoto Shoji Fukushima Nobuyuki Ito Takehiko Okamura Kazuo Ohtaguro Hiroto Washida Kimihiko Satoh Zahra Amelizad Franz Oesch 《Virchows Archiv. B, Cell pathology including molecular pathology》1988,56(1):307-316
The expression of a number of enzymes involved in drug metabolism, membrane function etc. was compared in hyperplastic and
neoplastic lesions of the rat bladder and in human bladder tumours. Transitional cell carcinomas (TCC) in both rat and Man
were characterized by decreased alkaline phosphatase (ALP) and increased gammaglutamyl transpeptidase (GGT), β-glucuronidase
(β-Gl), succinate dehydrogenase (SD) and glucose-6-phosphate dehydrogenase (G6PD) activities. In addition, binding for antibodies
specific for different cytochrome P-450 species (UT50, PB3a, MC1, MC2) and microsomal epoxide hydrolase (mEHb) was elevated in both murine and human tumours. Comparison of the enzyme phenotype
in hyperplastic lesions induced by freeze ulceration or uracil administration with that in preneoplastic papillary or nodular
hyperplasia (PNH) and TCC suggested, however, that most of the alteration in enzyme content or activity was non-specific and
related to requirements for epithelial cell proliferation. On the other hand, the decreased ALP, and increased GGT and β -
Gl activity appeared more directly related to neoplastic transformation. The results suggested that qualitative differences
exist between reactive hyperplasia and preneoplastic or neoplastic lesions in the urinary bladder. The finding of increased
cytochrome P-450, in clear contrast to the reduction characteristic of preneoplastic hepatic lesions, may be important with
regard to the observed difference in neoplastic transformation between the bladder and liver in response to drug metabolising
enzyme inducers.
Supported by Grants-in-aid for Cancer Research and a Comprehensive 10 Year Strategy for Cancer Control from the Ministry of
Health and Welfare, for Cancer Research and Scientific Research from the Ministry of Education, Science and Culture, and by
a grant from the Deutsche Forschungsgemeinschaft 相似文献