全文获取类型
收费全文 | 541篇 |
免费 | 40篇 |
出版年
2022年 | 1篇 |
2021年 | 4篇 |
2020年 | 5篇 |
2019年 | 2篇 |
2018年 | 11篇 |
2017年 | 9篇 |
2016年 | 13篇 |
2015年 | 13篇 |
2014年 | 22篇 |
2013年 | 33篇 |
2012年 | 28篇 |
2011年 | 23篇 |
2010年 | 22篇 |
2009年 | 29篇 |
2008年 | 38篇 |
2007年 | 37篇 |
2006年 | 40篇 |
2005年 | 46篇 |
2004年 | 49篇 |
2003年 | 41篇 |
2002年 | 35篇 |
2001年 | 5篇 |
2000年 | 10篇 |
1999年 | 8篇 |
1998年 | 8篇 |
1997年 | 7篇 |
1996年 | 3篇 |
1995年 | 6篇 |
1994年 | 4篇 |
1993年 | 6篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1980年 | 1篇 |
1978年 | 4篇 |
1977年 | 1篇 |
排序方式: 共有581条查询结果,搜索用时 46 毫秒
571.
Naoki Osumi Yoshihiro Kakehashi Shiho Matsumoto Kazunari Nagaoka Junichi Sakai Kiyotaka Miyashita Makoto Kimura Susumu Asakawa 《Archaea (Vancouver, B.C.)》2008,2(3):185-191
The gene sequences encoding disaggregatase (Dag), the enzyme
responsible for dispersion of cell aggregates of
Methanosarcina mazei to single cells, were determined
for three strains of M. mazei (S-6T, LYC
and TMA). The dag genes of the three strains were
3234 bp in length and had almost the same sequences with 97% amino
acid sequence identities. Dag was predicted to comprise 1077 amino
acid residues and to have a molecular mass of 120 kDa containing three
repeats of the DNRLRE domain in the C terminus, which is specific to
the genus Methanosarcina and may be responsible for
structural organization and cell wall function. Recombinant Dag was
overexpressed in Escherichia coli and preparations of
the expressed protein exhibited enzymatic activity. The RT-PCR
analysis showed that dag was transcribed to mRNA in
M. mazei LYC and indicated that the gene was
expressed in vivo. This is the first time the gene involved in the
morphological change of Methanosarcina spp. from
aggregate to single cells has been identified. 相似文献
572.
573.
Nobuyuki Morimoto Naruhito Ogino Tadashi Narita Kazunari Akiyoshi 《Journal of biotechnology》2009,140(3-4):246-249
An enzyme-responsive artificial chaperone system which employs an amphiphilic amylose primer (dodecyl maltopentaose, C12-MP) as a surfactant and phosphorylase b was designed to enable protein refolding. Effective refolding of carbonic anhydrase B after both heat denaturation (70 °C for 10 min) and guanidine hydrochloride (6 M) denaturation was observed by controlled association between the protein molecules and the C12-MP primer micelle through an enzymatic reaction. 相似文献
574.
Preeyanan Anwised Thai Kabbua Theeranan Temsiripong Apisak Dhiravisit Sarawut Jitrapakdee Tomohiro Araki Kazunari Yoneda Sompong Thammasirirak 《The protein journal》2013,32(3):172-182
The first report of complete nucleotide sequences for α- and β-globin chains from the Siamese hemoglobin (Crocodylus siamensis) is given in this study. The cDNAs encoding α- and β-globins were cloned by RT-PCR using the degenerate primers and by the rapid amplification of cDNA ends method. The full-length α-globin cDNA contains an open reading frame of 423 nucleotides encoding 141 amino acid residues, whereas the β-globin cDNA contains an open reading frame of 438 nucleotides encoding 146 amino acid residues. The authenticity of both α- and β-globin cDNA clones were also confirmed by the heterologous expression in Escherichia coli (E. coli). This is the first time that the recombinant C. siamensis globins were produced in prokaryotic system. Additionally, the heme group was inserted into the recombinant proteins and purified heme-bound proteins were performed by affinity chromatography using Co2+-charged Talon resins. The heme-bound proteins appeared to have a maximum absorbance at 415 nm, indicated that the recombinant proteins bound to oxygen and formed active oxyhemoglobin (HbO2). The results indicated that recombinant C. siamensis globins were successfully expressed in prokaryotic system and possessed an activity as ligand binding protein. 相似文献
575.
Yang Degang Takeshi Akama Takeshi Hara Kazunari Tanigawa Yuko Ishido Masaichi Gidoh Masahiko Makino Norihisa Ishii Koichi Suzuki 《PLoS neglected tropical diseases》2012,6(12)
Mycobacterium leprae (M. leprae) lives and replicates within macrophages in a foamy, lipid-laden phagosome. The lipids provide essential nutrition for the mycobacteria, and M. leprae infection modulates expression of important host proteins related to lipid metabolism. Thus, M. leprae infection increases the expression of adipophilin/adipose differentiation-related protein (ADRP) and decreases hormone-sensitive lipase (HSL), facilitating the accumulation and maintenance of lipid-rich environments suitable for the intracellular survival of M. leprae. HSL levels are not detectable in skin smear specimens taken from leprosy patients, but re-appear shortly after multidrug therapy (MDT). This study examined the effect of MDT components on host lipid metabolism in vitro, and the outcome of rifampicin, dapsone and clofazimine treatment on ADRP and HSL expression in THP-1 cells. Clofazimine attenuated the mRNA and protein levels of ADRP in M. leprae-infected cells, while those of HSL were increased. Rifampicin and dapsone did not show any significant effects on ADRP and HSL expression levels. A transient increase of interferon (IFN)-β and IFN-γ mRNA was also observed in cells infected with M. leprae and treated with clofazimine. Lipid droplets accumulated by M. leprae-infection were significantly decreased 48 h after clofazimine treatment. Such effects were not evident in cells without M. leprae infection. In clinical samples, ADRP expression was decreased and HSL expression was increased after treatment. These results suggest that clofazimine modulates lipid metabolism in M. leprae-infected macrophages by modulating the expression of ADRP and HSL. It also induces IFN production in M. leprae-infected cells. The resultant decrease in lipid accumulation, increase in lipolysis, and activation of innate immunity may be some of the key actions of clofazimine. 相似文献
576.
577.
Steroid hormones and their receptors in the brain 总被引:2,自引:0,他引:2
Mitsuhiro Kawata Kazunari Yuri Hitoshi Ozawa Mayumi Nishi Takao Ito Zhongting Hu Haiping Lu Maki Yoshida 《The Journal of steroid biochemistry and molecular biology》1998,65(1-6):273-280
Steroid hormones regulate several important functions of the brain by altering the expression of particular genes through their receptors. First in this paper the localization of glucocorticoid receptor immunoreactivity and mRNA in the brain was examined. Second biphasic effects of glucocorticoid on the hippocampus was described and particular emphasis was given on the apoptosis. Third the significance of estrogen receptor in the sexually dimorphic areas was discussed. These results suggest that steroids modulate the gene expression along with the alteration of cell structures in a different manner in a tissue-specific pattern. 相似文献
578.
Sixty-seven isolates of the southern blight fungus from Japan were divided into five groups based on ITS-RFLP analysis of
nuclear rDNA. Morphological characters of sclerotia varied between groups. Three groups were reidentified asSclerotium rolfsii, and two resembledS. delphinii in RFLP patterns and/or in having large sclerotia and relatively low optimal growth temperature (28°C). Sclerotia of the
latter, however, varied in size according to temperature and became indistinguishable from those ofS. rolfsii at high temperatures. Hyphal anastomosis (imperfect fusion) was observed between different ITS-RFLP groups, as well as between
different isolates belonging to the same groups. These results indicate that populations of this fungus in Japan consists
of several different subgroups, although morphological differences are not always evident. 相似文献
579.
580.
Lertvachirapaiboon Chutiparn Kiyokawa Itaru Baba Akira Shinbo Kazunari Kato Keizo 《Plasmonics (Norwell, Mass.)》2020,15(1):3-9
Plasmonics - Plasmonic nanostructures can be used to control the photoluminescence properties of various emitting materials. In this work, an efficient plasmon-induced energy transfer (quenching)... 相似文献