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81.
K Kaneko H Nishiyama K Ohba A Shibasaki T Hirose K Totsune K Furuyama K Takahashi 《Peptides》2012,37(2):285-289
The renin-angiotensin system is known to enhance erythropoiesis. (Pro)renin receptor ((P)RR), a specific receptor for renin and prorenin, has recently been identified. However, expression of (P)RR in erythroid cells has not been studied. The aim of the present study is to clarify expression of (P)RR in erythroid cells, and the effects of erythropoietin, angiotensin II, transforming growth factor-β1 (TGF-β1), interferon-γ (IFN-γ) and interleukin-1β (IL-1β) on its expression. Western blot analysis showed that (P)RR protein was expressed in human cultured erythroid cell lines, YN-1 and YN-1-0-A (a clonal variant cell line of YN-1). Erythropoietin (1IU/ml) increased (P)RR mRNA expression levels in YN-1-0-A cells (1.7-fold increase compared with control), but angiotensin II did not. Treatment of YN-1-0-A cells with IFN-γ (10ng/ml) for 48h increased the expression levels of (P)RR protein significantly (1.4-fold increase compared with control), whereas it had no significant effects on expression levels of (P)RR mRNA. Treatment of YN-1-0-A cells with TGF-β1 or IL-1β for 24 or 48h had no significant effects on expression levels of (P)RR. The present study has shown for the first time expression of (P)RR in erythroid cells, raising the possibility that (P)RR may have a role in erythropoiesis and the pathophysiology of certain types of anemia. 相似文献
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Alam M Nur-A-Hasan Ahsan S Pazhani GP Tamura K Ramamurthy T Gomes DJ Rahman SR Islam A Akhtar F Shinoda S Watanabe H Faruque SM Nair GB 《Microbiology and immunology》2006,50(5):359-370
Pathogenic Escherichia coli remains important etiological agent of infantile diarrhea in Bangladesh. Previous studies have focused mostly on clinical strains, but very little is known about their presence in aquatic environments. The present study was designed to characterize potentially pathogenic E. coli isolated between November 2001 and December 2003 from aquatic environments of 13 districts of Bangladesh. Serotyping of 96 randomly selected strains revealed O161 to be the predominant serotype (19%), followed by O55 and O44 (12% each), and 11% untypable. Serotype-based pathotyping of the E. coli strains revealed 47%, 30%, and 6% to belong to EPEC, ETEC, and EHEC pathotypes, respectively. The majority of the 160 strains tested were resistant to commonly used antimicrobial agents. Plasmid pro-filing showed a total of 17 different bands ranging from 1.3 to 40 kb. However, 35% of the strains did not contain any detectable plasmid, implying no correlation between plasmid and drug resistance. Although virulence gene profiling revealed 97 (61%) of the strains to harbor the gene encoding heat-stable enterotoxin (ST), 2 for the gene encoding Shiga toxin (Stx), and none for the gene for heat-labile enterotoxin (LT), serotype-based pathotyping of E. coli was not fully supported by this gene profiling. A dendrogram derived from the PFGE patterns of 22 strains of three predominant serogroups indicated two major clusters, one containing mainly serogroup O55 and the other O8. Three strains of identical PFGE profiles belonging to serogroup O55 were isolated from three distinct areas, which may be of epidemiological significance. Finally, it may be concluded that serotype-based pathotyping may be useful for E. coli strains of clinical origin; however, it is not precise enough for reliably identifying environmental strains as diarrheagenic. 相似文献
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Quantification of proton budgets in soils of cropland and adjacent forest in Thailand and Indonesia 总被引:1,自引:0,他引:1
Fujii Kazumichi Funakawa Shinya Hayakawa Chie Sukartiningsih Kosaki Takashi 《Plant and Soil》2009,315(1-2):241-255
Mechanisms for tolerance and avoidance of salinity at germination were examined in five self-regenerating annual pasture legumes of Mediterranean origin (Medicago polymorpha, Melilotus siculus, Trifolium subterraneum, T. michelianum and T. tomentosum). The maximum NaCl concentrations, for which no reduction in germination percentage occurred, were 300 mM for M. siculus, 240 mM for M. polymorpha and 120 mM for T. michelianum, T. subterraneum and T. tomentosum. Following 21 days exposure to 300 mM NaCl, imbibed seeds of T. subterraneum showed reduced germinability upon transfer to non-saline solution, whereas those of the four other species recovered full germinability. Following exposure to 600 mM NaCl, however, only T. michelianum and Melilotus siculus had some degree of recovery, with 38% and 31% germinability, respectively. Na+ in imbibed seed tissues of all species increased markedly with increasing NaCl concentration, while K+ decreased in all but Melilotus siculus. Seed coat impermeability (‘hard seeds’) acted as protection against the toxic effects of salinity. Melilotus siculus, Medicago polymorpha and T. tomentosum showed a delay in hard-seed breakdown (‘seed softening’) over the summer–autumn period, compared to T. subterraneum and T. michelianum, which acts to defer germination until soil surface salinity levels are likely to be lower. These three species also had higher levels of residual hard seeds. The results from this study support field observations that Melilotus siculus and Medicago polymorpha are the best adapted annual pasture legumes for highly saline soils in southern Australia, T. tomentosum and T. michelianum have some adaptation to moderately and mildly saline soils, respectively, whereas T. subterraneum has no adaptive traits for even mildly saline environments. A model for annual legume germination on saline land in southern Australia is also presented. 相似文献
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Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones (MICs of ciprofloxacin, 0.25 to 2 microg/ml) have a mutation at codon either Ser-83 or Asp-87 of gyrA gene. A screening method by PCR-restriction fragment length polymorphism (PCR-RFLP) was designed to screen the mutations at codon Ser-83 and Asp-87 of the gyrA gene of S. enterica serovar Typhi and serovar Paratyphi A clinical isolates. This method successfully screened the gyrA mutations of S. enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones. 相似文献
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Kanji Miki Kazumichi Tamura Riichi Sakazaki Yoshimasa Kosako 《Microbiology and immunology》1996,40(12):915-921
The antigenic scheme for the Bethesda-Ballerup group of bacteria established by West and Edwards in 1954 has continued to be applied as a serotyping scheme for Citrobacter freundii. In 1993, however, the classification of the Citrobacter was drastically revised and the species C. freundii redefined by Brenner et al. Accordingly, to judge the propriety to continuously use a single antigenic scheme for the C. freundii complex, the 90 reference strains listed in the antigenic scheme for C. freundii by West and Edwards were characterized phenotypically and specified based on the revised classification. Of these 90 strains, two strains of Hafnia alvei and one of Escherichia coli were found. Among the remaining 87 reference strains, Citrobacter youngae was the predominant species (40 strains), followed by Citrobacter braakii (25 strains), Citrobacter werkmanii (13 strains), and the unnamed Citrobacter genospecies 10 of Brenner et al (six strains). Citrobacter freundii, as redefined, accounted for only three strains and ranked behind the other four species. No overlapping with most of the 42 O-groups and 82 H-antigens was recognized between species with few exceptions. O-groups 1–9 inclusive, which were estimated to represent more than 90% of the former C. freundii strains, occurred in strains of C. youngae and C. braakii; and all nine strains of O-group 29, formerly known as the Ballerup group, were identified as C. braakii. These findings suggest that further study of the serotyping system is needed for all H2S-producing Citrobacter species. 相似文献
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Jinsen Zheng Alberto Canarini Kazumichi Fujii William N. Mmari Method M. Kilasara Shinya Funakawa 《Global Change Biology》2023,29(6):1514-1529
Sub-Saharan Africa (SSA) must undertake proper cropland intensification for higher crop yields while minimizing climate impacts. Unfortunately, no studies have simultaneously quantified greenhouse gas (GHG; CO2, CH4, and N2O) emissions and soil organic carbon (SOC) change in SSA croplands, leaving it a blind spot in the accounting of global warming potential (GWP). Here, based on 2-year field monitoring of soil emissions of CO2, CH4, and N2O, as well as SOC changes in two contrasting soil types (sandy vs. clayey), we provided the first, full accounting of GWP for maize systems in response to cropland intensifications (increasing nitrogen rates and in combination with crop residue return) in SSA. To corroborate our field observations on SOC change (i.e., 2-year, a short duration), we implemented a process-oriented model parameterized with field data to simulate SOC dynamic over time. We further tested the generality of our findings by including a literature synthesis of SOC change across maize-based systems in SSA. We found that nitrogen application reduced SOC loss, likely through increased biomass yield and consequently belowground carbon allocation. Residue return switched the direction of SOC change from loss to gain; such a benefit (SOC sequestration) was not compromised by CH4 emissions (negligible) nor outweighed by the amplified N2O emissions, and contributed to negative net GWP. Overall, we show encouraging results that, combining residue and fertilizer-nitrogen input allowed for sequestering 82–284 kg of CO2-eq per Mg of maize grain produced across two soils. All analyses pointed to an advantage of sandy over clayey soils in achieving higher SOC sequestration targets, and thus call for a re-evaluation on the potential of sandy soils in SOC sequestration across SSA croplands. Our findings carry important implications for developing viable intensification practices for SSA croplands in mitigating climate change while securing food production. 相似文献
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The gene encoding FtZ was cloned from triangular disc-shaped extremely halophilic archaeon Haloarcula japonica strain TR-1. Nucleotide sequencing analysis of the possible ftsZ gene revealed that the structural gene consisted of an open reading frame of 1,182 nucleotides encoding 394 amino acids. The deduced amino acid sequence of the Ha. japonica FtsZ showed high identities with those Halobacterium salinarom, Haloferax volcanii and Haloferax mediterranei FtsZs. 相似文献
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Yue Li Kazumichi Fujiwara Naoki Osada Yosuke Kawai Toyoyuki Takada Alexey P. Kryukov Kuniya Abe Hiromichi Yonekawa Toshihiko Shiroishi Kazuo Moriwaki Naruya Saitou Hitoshi Suzuki 《Heredity》2021,126(1):132
The Eurasian house mouse Mus musculus is useful for tracing prehistorical human movement related to the spread of farming. We determined whole mitochondrial DNA (mtDNA) sequences (ca. 16,000 bp) of 98 wild-derived individuals of two subspecies, M. m. musculus (MUS) and M. m. castaneus (CAS). We revealed directional dispersals reaching as far as the Japanese Archipelago from their homelands. Our phylogenetic analysis indicated that the eastward movement of MUS was characterised by five step-wise regional extension events: (1) broad spatial expansion into eastern Europe and the western part of western China, (2) dispersal to the eastern part of western China, (3) dispersal to northern China, (4) dispersal to the Korean Peninsula and (5) colonisation and expansion in the Japanese Archipelago. These events were estimated to have occurred during the last 2000–18,000 years. The dispersal of CAS was characterised by three events: initial divergences (ca. 7000–9000 years ago) of haplogroups in northernmost China and the eastern coast of India, followed by two population expansion events that likely originated from the Yangtze River basin to broad areas of South and Southeast Asia, including Sri Lanka, Bangladesh and Indonesia (ca. 4000–6000 years ago) and to Yunnan, southern China and the Japanese Archipelago (ca. 2000–3500). This study provides a solid framework for the spatiotemporal movement of the human-associated organisms in Holocene Eastern Eurasia using whole mtDNA sequences, reliable evolutionary rates and accurate branching patterns. The information obtained here contributes to the analysis of a variety of animals and plants associated with prehistoric human migration.Subject terms: Evolution, Genetic variation 相似文献