全文获取类型
收费全文 | 8755篇 |
免费 | 545篇 |
国内免费 | 7篇 |
专业分类
9307篇 |
出版年
2022年 | 36篇 |
2021年 | 81篇 |
2020年 | 43篇 |
2019年 | 55篇 |
2018年 | 82篇 |
2017年 | 89篇 |
2016年 | 123篇 |
2015年 | 184篇 |
2014年 | 222篇 |
2013年 | 497篇 |
2012年 | 407篇 |
2011年 | 405篇 |
2010年 | 270篇 |
2009年 | 274篇 |
2008年 | 414篇 |
2007年 | 441篇 |
2006年 | 413篇 |
2005年 | 419篇 |
2004年 | 413篇 |
2003年 | 388篇 |
2002年 | 368篇 |
2001年 | 346篇 |
2000年 | 347篇 |
1999年 | 283篇 |
1998年 | 139篇 |
1997年 | 131篇 |
1996年 | 94篇 |
1995年 | 96篇 |
1994年 | 84篇 |
1993年 | 79篇 |
1992年 | 217篇 |
1991年 | 174篇 |
1990年 | 163篇 |
1989年 | 177篇 |
1988年 | 160篇 |
1987年 | 122篇 |
1986年 | 119篇 |
1985年 | 110篇 |
1984年 | 89篇 |
1983年 | 78篇 |
1982年 | 51篇 |
1981年 | 47篇 |
1980年 | 54篇 |
1979年 | 56篇 |
1978年 | 59篇 |
1977年 | 43篇 |
1976年 | 36篇 |
1975年 | 36篇 |
1974年 | 46篇 |
1973年 | 51篇 |
排序方式: 共有9307条查询结果,搜索用时 0 毫秒
941.
942.
Tajino K Matsumura K Kosada K Shibakusa T Inoue K Fushiki T Hosokawa H Kobayashi S 《American journal of physiology. Regulatory, integrative and comparative physiology》2007,293(5):R2128-R2135
When ambient temperature is decreased in mammals, autonomic and behavioral heat-gain responses occur to maintain their core temperatures. However, what molecules in cutaneous sensory nerve endings mediate cooling-induced responses is unclear. Recently, transient receptor potential melastatin-8 (TRPM8) has been identified in cell bodies of sensory neurons as low-temperature and menthol-activated cation channel. We hypothesized that TRPM8 mediates cooling-induced autonomic and behavioral heat-gain responses. To activate TRPM8 specifically, we applied 1-10% menthol to the skin of whole trunk in mice instead of cooling and measured core temperatures and autonomic and behavioral heat-gain responses. Solvent of menthol (100% ethanol) was used as control. Significant elevation of core temperatures was observed between 20 and 120 min after menthol application. Pretreatment with diclofenac sodium, an antipyretic drug, did not affect this hyperthermia, indicating that the menthol-induced hyperthermia is not fever. Menthol application induced a rise in oxygen consumption, shivering-like muscle activity, tail skin vasoconstriction (autonomic responses), and heat-seeking behavior. All of them are typical heat-gain responses. These results support the hypothesis that TRPM8 mediates cooling-induced autonomic and behavioral heat-gain responses. 相似文献
943.
Ohshita K Ishiyama H Oyanagi K Nakata H Kobayashi J 《Bioorganic & medicinal chemistry》2007,15(9):3235-3240
Four hybrid molecules (1 and 12-14) of caffeine and eudistomin D, a beta-carboline alkaloid from a marine tunicate, were synthesized, and their affinity and selectivity for adenosine receptors A(1), A(2A), and A(3) were examined. It was found that all the compounds showed better potency as adenosine receptor ligands as compared with caffeine. Among them, a compound (13) possessing a nitrogen at the delta-position of the pyridine ring (delta-N type) showed the most potent affinity for adenosine receptor A(3) subtype, while N-methylation (14) of a pyrrole ring in 13 significantly lowered the potency as adenosine receptor ligands. Compounds (1 and 12) having a nitrogen at the beta-position of the pyridine ring (beta-N type) showed lower affinity than the corresponding delta-N type compounds (13 and 14), while compounds (10, 11, and 17) lacking a pyrrole ring between the pyridine and pyrimidine rings exhibited almost no affinity to the adenosine receptor subtypes examined. 相似文献
944.
Structure-activity relationship and biological property of cortistatins, anti-angiogenic spongean steroidal alkaloids 总被引:1,自引:0,他引:1
Aoki S Watanabe Y Tanabe D Arai M Suna H Miyamoto K Tsujibo H Tsujikawa K Yamamoto H Kobayashi M 《Bioorganic & medicinal chemistry》2007,15(21):6758-6762
Previously, bioassay-guided separation led us to isolate eleven novel steroidal alkaloids named cortistatins from the marine sponge Corticium simplex. These cortistatins were classified into three types based on the chemical structure of the side chain part, that is, isoquinoline, N-methyl piperidine or 3-methylpyridine units. From the structure-activity relationship study, the isoquinoline unit in the side chain was found to be crucial for the anti-angiogenic activity of cortistatins. Cortistatin A (1) showed cytostatic growth-inhibitory activity against human umbilical vein endothelial cells (HUVECs). Cortistatin A (1) also inhibited VEGF-induced migration of HUVECs and bFGF-induced tubular formation. Although cortistatin A (1) showed no effect on VEGF-induced phosphorylation of ERK1/2 and p38, which are one of the signaling pathways for migration and tubular formation, the phosphorylation of the unidentified 110kDa protein in HUVECs was inhibited by the treatment with cortistatin A. 相似文献
945.
Kobayashi E Shimazaki M Miyamoto Y Masuno H Yamamoto K DeLuca HF Yamada S Shimizu M 《Bioorganic & medicinal chemistry》2007,15(3):1475-1482
We have synthesized four new geometric isomers of 1alpha,25-dihydroxy-2-(2'-fluoroethylidene)-19-norvitamin D analogs 1 and 2 having a 20R- and 20S-configuration, whose structures are correlated with 2MD possessing high potencies in stimulating bone formation in vitro and in vivo. The E-isomers of (20R)- and (20S)-2-fluoroethylidene analogs 1a and 1b were comparable with the natural hormone 1alpha,25-(OH)(2)D(3) in binding to the vitamin D receptor (VDR), while two Z-isomers 2a and 2b were about 15-20 times less active than the hormone. In inducing expression of the vitamin D responsive element-based luciferase reporter gene, the E-isomers 1a and 1b were 1.2- and 8.6-fold more potent than the hormone, respectively, while the Z-isomers 2a and 2b had 27-55% of the potency. On the basis of the biological activities and a docking simulation based on X-ray crystallographic analysis of the VDR ligand-binding pocket, the structure-activity relationships of the fluorinated 19-norvitamin D analogs are discussed. 相似文献
946.
947.
In South America, the largest savanna region is the Brazilian cerrado, in which there are few areas that become waterlogged in the rainy season. However, we found a small cerrado area in which the soil is poorly drained and becomes waterlogged at the end of the rainy season, allowing the appearance of a hyperseasonal cerrado. We investigated the soil–vegetation relationships in three vegetation forms: hyperseasonal cerrado, seasonal cerrado, and wet grassland. We collected vegetation and soil samples in these three vegetation forms and submitted obtained data to a canonical correspondence analysis. Our results showed a distinction among hyperseasonal cerrado, seasonal cerrado and wet grassland, which presented different floristic compositions and species abundances. The edaphic variables best related to the hyperseasonal and seasonal cerrados were sand, base saturation, pH, and magnesium. The wet grassland was related to higher concentrations of clay, organic matter, aluminium saturation, aluminium, phosphorus, and potassium. Although it is not possible to infer causal relationships based on our results, we hypothesize that the duration of waterlogging in the hyperseasonal cerrado may not be long enough to alter most of its soil characteristics, such as organic matter, phosphorus, and potassium, but may be long enough to alter some, such as pH and base saturation, as the soils under both cerrados were more similar to one another than to the soil under the wet grassland. Since waterlogging may alter soil characteristics and since these characteristics were enough to explain the plant community variation, we may conclude that water excess—permanent or seasonal—is one of the main factors to distinguish the three vegetation forms, which presented different floristic compositions and species abundances. 相似文献
948.
Aoki S Morohashi K Sunoki T Kuramochi K Kobayashi S Sugawara F 《Bioconjugate chemistry》2007,18(6):1981-1986
Paclitaxel (Taxol), an effective anticancer agent, is known to bind to tubulin and induce tubulin polymerization. Several other binding proteins of paclitaxel, such as Bcl-2, heat shock proteins, and NSC-1, have also been reported. Here, we describe a T7 phage-based display to screen for paclitaxel-binding molecules from a random peptide library using paclitaxel-photoimmobilized TentaGel resin. Specific phage particles that bind the paclitaxel-immobilized resin were obtained. Among them, two phage clones included the same consensus amino acid sequence (KACGRTRVTS). Analysis of the protein database using BLAST revealed that a portion of this sequence is conserved in the zinc finger domain of human NFX1. Binding affinity of paclitaxel against the partial recombinant protein of NFX1 (424aa-876aa) was confirmed by pull-down assays and surface plasmon resonance analyses. 相似文献
949.
To assess delayed fertility in male growth-retarded (grt) mice with congenital primary hypothyroidism, their testes were chronologically examined. The testicular weight in grt mice was significantly lower than age-matched normal mice until 8 weeks but was comparable at 13 and 26 weeks. While normal mice had mature sperm cells in both testes and epididymides at 5 weeks, age-matched grt mice did not. The size of the seminiferous tubules in testes of grt mice was smaller than that of normal mice before 13 weeks but was comparable at 26 weeks. These findings suggest that male grt mice might need more than 13 weeks to develop mature testes. 相似文献
950.
Shi R Zhang J Li C Kazumi Y Sugawara I 《Microbes and infection / Institut Pasteur》2007,9(14-15):1538-1544
China is regarded by the World Health Organization as a major hot-spot region for Mycobacterium tuberculosis infection. Streptomycin has been deployed in China for over 50 years and is still widely used for tuberculosis treatment. We have developed a denaturing HPLC (DHPLC) method for detecting various gene mutations conferring drug resistance in M. tuberculosis. The present study focused on rpsL and rrs mutation analysis. Two hundred and fifteen M. tuberculosis clinical isolates (115 proved to be streptomycin-resistant and 100 susceptible by a routine proportional method) from China were tested to determine the streptomycin minimal inhibitory concentration (MIC), and subjected to DHPLC and concurrent DNA sequencing to determine rpsL and rrs mutations. The results showed that 85.2% (98/115) of streptomycin-resistant isolates harbored rpsL or rrs mutation, while rpsL mutation (76.5%, 88/115) dominated. MIC of 98 mutated isolates revealed no close correlation between mutation types and levels of streptomycin resistance. No mutation was found in any of the susceptible isolates. The DHPLC results were completely consistent with those of sequencing. The DHPLC method devised in this study can be regarded as a useful and powerful tool for detection of streptomycin resistance. This is the first report to describe DHPLC analysis of mutations in the rpsL and rrs genes of M. tuberculosis in a large number of clinical isolates. 相似文献