全文获取类型
收费全文 | 811篇 |
免费 | 66篇 |
出版年
2023年 | 3篇 |
2022年 | 7篇 |
2021年 | 18篇 |
2020年 | 8篇 |
2019年 | 3篇 |
2018年 | 13篇 |
2017年 | 10篇 |
2016年 | 21篇 |
2015年 | 28篇 |
2014年 | 23篇 |
2013年 | 38篇 |
2012年 | 38篇 |
2011年 | 54篇 |
2010年 | 19篇 |
2009年 | 21篇 |
2008年 | 36篇 |
2007年 | 41篇 |
2006年 | 34篇 |
2005年 | 39篇 |
2004年 | 29篇 |
2003年 | 41篇 |
2002年 | 48篇 |
2001年 | 34篇 |
2000年 | 39篇 |
1999年 | 22篇 |
1998年 | 15篇 |
1997年 | 9篇 |
1996年 | 8篇 |
1995年 | 6篇 |
1994年 | 12篇 |
1993年 | 9篇 |
1992年 | 16篇 |
1991年 | 18篇 |
1990年 | 25篇 |
1989年 | 17篇 |
1988年 | 21篇 |
1987年 | 14篇 |
1986年 | 4篇 |
1985年 | 9篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 3篇 |
1981年 | 3篇 |
1979年 | 4篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1970年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有877条查询结果,搜索用时 15 毫秒
21.
Akemi Tsuda Masatoshi Ito Kazuko Kishi Hideyuki Shiraishi Hideo Tsuda Chuzo Mori 《Neurochemical research》1994,19(1):1-4
To investigate the mechanism of penicillin-induced convulsions, we have studied the effects of penicillin G (PC-G) on GABA-gated chloride ion influx in brain microsac preparations of mice. In the presence of 10–4 M GABA, PC-G inhibited GABA-gated chloride ion influx in a dose-dependent manner. The dose-response curve for GABA in the presence of 10–3 M PC-G was shifted rightward and there was a decrease in maximum response. The inhibitory effects of PC-G were not reversed by RO 15-1788, an antagonist of benzodiazepine (BZ) receptors, but were reversed by washing the microsac membranes. Therefore, PC-G probably exerts its proconvulsant effect by inhibiting GABA-gated chloride ion influx. However, it appears not to act through the BZ receptor of the GABA/BZ receptor complex. 相似文献
22.
Bulb canker of garlic caused byEmbellisia allii was newly detected in Japan. Symptoms of the disease are described and the morphological characteristics of the causal fungus are illustrated and described. 相似文献
23.
24.
S Ito A Iwasaki J Syundo Y Tamura S Kishi H Mori K Ii Y Matsuda N Katsunuma 《The journal of histochemistry and cytochemistry》1989,37(5):611-615
Human liver guanase was purified and a specific antibody against it was raised in rabbits. The antiserum formed a single precipitin line with human liver extract, and also completely inhibited the activity of the liver enzyme. An immunoblotting study showed that the antibody bound specifically to one band of protein with guanase activity and not to other proteins. Therefore, we concluded that this antiserum against the liver enzyme was suitable for use in immunohistochemical demonstration of guanase. In tissue sections, the immunohistochemical reaction with this antibody was positive in the same locations as the histochemical guanase reaction with DAB (3,3'-diaminobenzidine tetrahydrochloride). 相似文献
25.
Takedomi T Aoyagi Y Konishi M Kishi H Taya K Watanabe G Sasamoto S 《Theriogenology》1995,43(7):1259-1268
This study was undertaken to determine whether a single injection of porcine FSH (pFSH) would induce a superovulatory response in cattle. Holstein heifers were given a single injection of pFSH (30 mg, s.c.) dissolved in saline (Group 1, n = 5); 50% polyvinylpyrrolidone (PVP; Group 2, n = 5); or 25% PVP (Group 3, n = 4). Group-4 heifers (n = 5) were given multiple intramuscular injections of pFSH every 12 h for 3 d at decreasing doses, for a total of 30 mg. All animals received a single injection of 750 microg PGF2 alpha 48 h after the initiation of pFSH treatment. Animals exhibiting estrus were artificially inseminated twice throughout estrus. Ova and embryos were recovered nonsurgically. Ovaries were examined by transrectal ultrasonography or by palpation per rectum on Day 7 or 8 of estrus. Plasma concentrations of pFSH, bovine FSH progesterone, estradiol-17 beta and inhibin were determined by specific radioimmunoassays. The number of corpora lutea (CL) and the numbers of total and transferable embryos which were detected and recovered in Groups 2 and 3 were equivalent to the numbers detected and recovered in Group 4. In Group 1, however, only 1 of 5 animals ovulated even a single oocyte. The present study demonstrated that only a single injection of pFSH dissolved in PVP was capable of inducing a superovulatory response by maintaining a high plasma FSH concentration to allow for the recovery of a sufficient number of embryos for transplantation. 相似文献
26.
Enzymic analyses of aqueous extracts of barley obtained at 25–100° demonstrated an exponential relationship between β-d-glucan content and temperature. Purified β-d-glucans, in particular those from extracts made at 40° and 100°, were compared by the Smith-degradation method followed by g.l.c. analysis of the methyl and the trimethylsilyl ethers of the products. All extracts yielded qualitatively the same products, including oligosaccharides which were characteristic of sequences of adjacent (1→3)-linkages. The material extracted at 100° contained relatively more of these sequences, and also showed a much higher specific viscosity, than did the material extracted at low temperatures. The structural implications of these findings are discussed. 相似文献
27.
28.
29.
Y Suminami F Kishi K Sekiguchi H Kato 《Biochemical and biophysical research communications》1991,181(1):51-58
We have cloned cDNA of squamous cell carcinoma antigen. Sequence analysis of the complete 1711 basepairs SCC antigen cDNA revealed that it coded 390 amino acids and no typical signal sequence in the NH2-terminus. Northern blot analysis of human squamous cell poly(A)+ RNA using this cDNA insert as the probe showed a single RNA species of about 1.7 kilobases. The cDNA was expressed in Escherichia coli and the product was detected by immunological methods using antibodies against SCC antigen, indicating that this cDNA encodes the entire SCC antigen sequence. The amino acid homology search revealed that SCC antigen was a member of the serine protease inhibitors family. 相似文献
30.