全文获取类型
收费全文 | 1102篇 |
免费 | 61篇 |
国内免费 | 2篇 |
出版年
2023年 | 9篇 |
2022年 | 8篇 |
2021年 | 13篇 |
2020年 | 6篇 |
2019年 | 10篇 |
2018年 | 18篇 |
2017年 | 21篇 |
2016年 | 24篇 |
2015年 | 39篇 |
2014年 | 40篇 |
2013年 | 104篇 |
2012年 | 61篇 |
2011年 | 71篇 |
2010年 | 55篇 |
2009年 | 38篇 |
2008年 | 41篇 |
2007年 | 51篇 |
2006年 | 51篇 |
2005年 | 41篇 |
2004年 | 73篇 |
2003年 | 55篇 |
2002年 | 39篇 |
2001年 | 9篇 |
2000年 | 8篇 |
1999年 | 10篇 |
1998年 | 11篇 |
1997年 | 17篇 |
1996年 | 17篇 |
1995年 | 20篇 |
1994年 | 25篇 |
1993年 | 16篇 |
1992年 | 11篇 |
1991年 | 15篇 |
1990年 | 10篇 |
1988年 | 11篇 |
1987年 | 8篇 |
1985年 | 10篇 |
1984年 | 6篇 |
1982年 | 8篇 |
1981年 | 11篇 |
1980年 | 5篇 |
1979年 | 6篇 |
1978年 | 4篇 |
1977年 | 11篇 |
1976年 | 8篇 |
1975年 | 6篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1972年 | 3篇 |
1970年 | 4篇 |
排序方式: 共有1165条查询结果,搜索用时 734 毫秒
101.
A naturally occurring functional allele of the rice waxy locus has a GT to TT mutation at the 5' splice site of the first intron 总被引:7,自引:0,他引:7
102.
Yoshikazu Horie Dong Mei Li Toshimitu Fukiharu Ruoyu Li Paride Abliz Kazuko Nishimura Duan Li Wang 《Mycoscience》1998,39(2):161-165
Emericella appendiculata, a new species isolated from soil of the Pamire Plateau, is described and illustrated. It is characterized by grayish green
non-ostiolate ascomata surrounded by a thick layer of hülle cells, membranaceous peridium, prototunicate asci, violet-brown,
lenticular ascospores which are ornamented by two stellate equatorial crests, capitate convex surfaces, and long filiform
appendages, and anAspergillus anamorph with biseriate conidiogenous cells. 相似文献
103.
The changes and biochemical features of the epidermis that accompany the differentiation and embryonic shedding complex formation in grass snake Natrix natrix L. embryos were studied ultrastructurally and immunocytochemically with two panels of antibodies (AE1, AE3, AE1/AE3; anti-cytokeratin, pan mixture, Lu-5 and PCK-26). All observed changes in the ultrastructure of the cells forming the epidermal layers were associated with the physiological changes that occurred in the embryonic epidermis, such as changing of the manner of nutrition and keratinization leading to the embryonic shedding complex formation. The layers that originated first (basal, outer and inner periderm and clear layer) differentiated very early and rapidly. Rapid differentiation was also observed in the layers that are very important for the functioning of the epidermis in Natrix embryos (oberhäutchen and beta-layers). They started to differentiate at developmental stage IX, and then fused and formed the embryonic shedding complex at developmental stage XI. During the embryonic development of the grass snake the smallest changes appeared in the ultrastructure of the cells in the mesos and alpha-layers because they perform supplementary functions in the process of embryonic molting. They were undifferentiated until the end of embryonic development and started to differentiate just before the first adult molting. AE1/AE3, anti-cytokeratin, pan mixture, Lu-5 and PCK-26 antibodies immunolabeled clear layer, oberhäutchen and beta-layers at the latest phase of developmental stage XI. It should be noted that these antibodies did not immunolabel the alpha-layer until hatching. The presence of alpha-keratin immunolabeling in layers that were keratinized, particularly in the oberhäutchen and beta-layers in embryos, indicated that they were not as hard as in fully mature individuals. 相似文献
104.
105.
Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes 总被引:4,自引:0,他引:4
106.
Subterranean rodents continuously extend their burrow systems primarily in search of food, which has an important impact on
the ecosystem in which they live. Excavated soil may be pushed either into aboveground mounds or into tunnels underground.
Factors affecting the amount of burrowing and the preference of aboveground or underground soil deposition are, nevertheless,
little known. We investigated the influence of food supply, soil hardness, and the animal’s body mass on the mode of soil
deposition in ten burrow systems of free ranging silvery mole-rats Heliophobius argenteocinereus Peters, 1846. In each burrow system, we estimated the volume of backfilled tunnels and the volume of soil deposited aboveground.
The highest amount of variation in these parameters was explained by the interaction of food supply and soil hardness. The
ratio of the volume of backfilled tunnels to the volume of mounds was not significantly dependent on any of the explanatory
variables. The proportion of backfilled tunnels decreased with the increasing volume of the complete burrow system. We propose
that both low food supply and soft soil lead to an increased amount of burrowing, which results in a larger volume of soil
deposited both above ground and under ground over a given period of time. 相似文献
107.
PATRICIA FERNANDES NERES VERÔNICA GOMES DA FONSECA‐GENEVOIS RODRIGO AUGUSTO TORRES MARIANA DA FONSECA CAVALCANTI FRANCISCO JOSÉ VICTOR DE CASTRO NEYVAN RENATO RODRIGUES DA SILVA TANIA TASSINARI RIEGER WILFRIDA DECRAEMER 《Zoological Journal of the Linnean Society》2010,158(1):1-15
A new species of Daptonema is described based upon morphological characters and 18S rRNA sequence. Daptonema matrona sp. nov. was collected in Pina Basin (north‐eastern Brazil). It differs from all other species of the genus by the presence of reduced cephalic setae and straight spicules. These features require an adaptation of the generic diagnosis. Moreover, the females are characterized by intra‐uterine development of the offspring, considered herein as their major autapomorphic feature. Molecular systematic analyses supported Daptonema matrona sp. nov. as a distinct genetic and evolutionary lineage. The data also indicate hypotheses of taxonomic synonymies amongst some related taxa from Xyalidae as well as the paraphyly of Daptonema. © 2010 The Linnean Society of London, Zoological Journal of the Linnean Society, 2010, 158 , 1–15. 相似文献
108.
Nakayama T Yamazumi K Uemura T Yoshizaki A Yakata Y Matsuu-Matsuyama M Shichijo K Sekine I 《Radiation research》2007,168(4):433-439
X rays are well known to cause genetic damage and to induce many types of carcinomas in humans. The Apc(min/+) mouse, an animal model for human familial adenomatous polyposis (FAP), contains a truncating mutation in the APC gene and spontaneously develops intestinal adenomas. To elucidate the role of X rays in the development of intestinal tumors, we examined the promotion of carcinogenesis in X-irradiated Apc(min/+) mice. Forty out of 77 (52%) X-irradiated Apc(min/+) mice developed adenocarcinomas that invaded the proprial muscle layer of the small intestine; 24 of 44 (55%) were in males, and 16 of 33 (49%) were in females. In contrast, invasive carcinomas were detected in the small intestines of only 13 of 64 (20%) nonirradiated Apc(min/+) mice; nine of 32 (28%) were in males and four of 32 (13%) were in females. These differences between X-irradiated and nonirradiated Apc(min/+) mice in the occurrence of invasive intestinal carcinomas were statistically significant (P < 0.05 for males, P < 0.005 for females). In wild-type mice, invasive carcinomas were not detected in either X-irradiated or nonirradiated mice. Apc(min/+) mice had many polyps in the large intestine with or without X irradiation; there was no difference in the number of polyps between the two groups. Also, invasive carcinomas were not detected in the large intestine with or without irradiation. The occurrence of mammary tumors, which was observed in Apc(min/+) mice, was found to be increased in irradiated Apc(min/+) mice (P < 0.01). Apc(min/+) mice had many polyps in the small and large intestines with or without X irradiation. X-irradiated Apc(min/+) mice had highly invasive carcinomas in the small intestine with multiplicities associated with invasiveness. Our results suggest that X radiation may promote the invasive activity of intestinal tumors in Apc(min/+) mice. 相似文献
109.
Kanda Y Yamada T Mori K Okazaki A Inoue M Kitajima-Miyama K Kuni-Kamochi R Nakano R Yano K Kakita S Shitara K Satoh M 《Glycobiology》2007,17(1):104-118
The structure of asparagine-linked oligosaccharides attached to the antibody constant region (Fc) of human immunoglobulin G1 (IgG1) has been shown to affect the pharmacokinetics and antibody effector functions of antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). However, it is still unclear how differences in the N-linked oligosaccharide structures impact the biological activities of antibodies, especially those lacking core fucose. Here, we succeeded in generating core fucose-lacking human IgG1 antibodies with three different N-linked Fc oligosaccharides, namely, a high-mannose, hybrid, and complex type, using the same producing clone, and compared their activities. Cultivation of an alpha-1,6-fucosyltransferase (FUT8) knockout Chinese hamster ovary cell line in the presence or absence of a glycosidase inhibitor (either swainsonine or kifunensine) yielded antibody production of each of the three types without contamination by the others. Two of three types of nonnaturally occurring atypical oligosaccharide IgG1, except the complex type, reduced the affinity for both human lymphocyte receptor IIIa (FcgammaRIIIa) and the C1q component of the complement, resulting in reduction of ADCC and CDC. The bulky structure of the nonreducing end of N-linked Fc oligosaccharides is considered to contribute the CDC change, whereas the structural change in the reducing end, i.e. the removal of core fucose, causes ADCC enhancement through improved FcgammaRIIIa binding. In the pharmacokinetic profile, although no significant difference of human neonatal Fc receptor (FcRn)-binding affinity was observed among the three types, the complex type showed longer serum half-lives than the other types irrespective of core fucosylation in mice, which also suggests the contribution of the nonreducing end structure. The present study provides basic information on the effects of core fucose-lacking N-linked Fc oligosaccharides on antibody biological activities. 相似文献
110.
Receptor-like protein kinase 2 (RPK 2) is a novel factor controlling anther development in Arabidopsis thaliana 总被引:4,自引:0,他引:4
Mizuno S Osakabe Y Maruyama K Ito T Osakabe K Sato T Shinozaki K Yamaguchi-Shinozaki K 《The Plant journal : for cell and molecular biology》2007,50(5):751-766
Receptor-like kinases (RLK) comprise a large gene family within the Arabidopsis genome and play important roles in plant growth and development as well as in hormone and stress responses. Here we report that a leucine-rich repeat receptor-like kinase (LRR-RLK), RECEPTOR-LIKE PROTEIN KINASE2 (RPK2), is a key regulator of anther development in Arabidopsis. Two RPK2 T-DNA insertional mutants (rpk2-1 and rpk2-2) displayed enhanced shoot growth and male sterility due to defects in anther dehiscence and pollen maturation. The rpk2 anthers only developed three cell layers surrounding the male gametophyte: the middle layer was not differentiated from inner secondary parietal cells. Pollen mother cells in rpk2 anthers could undergo meiosis, but subsequent differentiation of microspores was inhibited by tapetum hypertrophy, with most resulting pollen grains exhibiting highly aggregated morphologies. The presence of tetrads and microspores in individual anthers was observed during microspore formation, indicating that the developmental homeostasis of rpk2 anther locules was disrupted. Anther locules were finally crushed without stomium breakage, a phenomenon that was possibly caused by inadequate thickening and lignification of the endothecium. Microarray analyses revealed that many genes encoding metabolic enzymes, including those involved in cell wall metabolism and lignin biosynthesis, were downregulated throughout anther development in rpk2 mutants. RPK2 mRNA was abundant in the tapetum of wild-type anthers during microspore maturation. These results suggest that RPK2 controls tapetal cell fate by triggering subsequent tapetum degradation, and that mutating RPK2 impairs normal pollen maturation and anther dehiscence due to disruption of key metabolic pathways. 相似文献