全文获取类型
收费全文 | 2888篇 |
免费 | 153篇 |
出版年
2023年 | 9篇 |
2022年 | 17篇 |
2021年 | 39篇 |
2020年 | 17篇 |
2019年 | 30篇 |
2018年 | 49篇 |
2017年 | 26篇 |
2016年 | 55篇 |
2015年 | 84篇 |
2014年 | 106篇 |
2013年 | 185篇 |
2012年 | 230篇 |
2011年 | 210篇 |
2010年 | 128篇 |
2009年 | 109篇 |
2008年 | 202篇 |
2007年 | 207篇 |
2006年 | 185篇 |
2005年 | 172篇 |
2004年 | 203篇 |
2003年 | 169篇 |
2002年 | 197篇 |
2001年 | 20篇 |
2000年 | 23篇 |
1999年 | 25篇 |
1998年 | 31篇 |
1997年 | 33篇 |
1996年 | 38篇 |
1995年 | 29篇 |
1994年 | 30篇 |
1993年 | 25篇 |
1992年 | 16篇 |
1991年 | 20篇 |
1990年 | 12篇 |
1989年 | 13篇 |
1988年 | 12篇 |
1987年 | 13篇 |
1986年 | 7篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 10篇 |
1982年 | 9篇 |
1981年 | 9篇 |
1980年 | 10篇 |
1979年 | 6篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 4篇 |
1970年 | 2篇 |
1969年 | 2篇 |
排序方式: 共有3041条查询结果,搜索用时 15 毫秒
991.
992.
Itoh Kazuhiro Iwasaki Hiromichi Negoro Eiju Shigemi Hiroko Tokimatsu Issei Tsutani Hiroshi Yamauchi Takahiro 《Mycopathologia》2021,186(1):113-117
Mycopathologia - Invasive trichosporonosis is a rare and lethal fungal infection that occurs in immunocompromised patients. Breakthrough trichosporonosis can occur in patients treated with... 相似文献
993.
Kentaro Ide Sanai Takahashi Keiko Sakai Yuki Taga Tomonori Ueno David Dickens Rosalind Jenkins Francesco Falciani Takako Sasaki Kazuhiro Ooi Shuichi Kawashiri Kazunori Mizuno Shunji Hattori Takao Sakai 《The Journal of biological chemistry》2021,297(1)
Collagen-derived hydroxyproline (Hyp)-containing peptides have a variety of biological effects on cells. These bioactive collagen peptides are locally generated by the degradation of endogenous collagen in response to injury. However, no comprehensive study has yet explored the functional links between Hyp-containing peptides and cellular behavior. Here, we show that the dipeptide prolyl-4-hydroxyproline (Pro-Hyp) exhibits pronounced effects on mouse tendon cells. Pro-Hyp promotes differentiation/maturation of tendon cells with modulation of lineage-specific factors and induces significant chemotactic activity in vitro. In addition, Pro-Hyp has profound effects on cell proliferation, with significantly upregulated extracellular signal–regulated kinase phosphorylation and extracellular matrix production and increased type I collagen network organization. Using proteomics, we have predicted molecular transport, cellular assembly and organization, and cellular movement as potential linked-network pathways that could be altered in response to Pro-Hyp. Mechanistically, cells treated with Pro-Hyp demonstrate increased directional persistence and significantly increased directed motility and migration velocity. They are accompanied by elongated lamellipodial protrusions with increased levels of active β1-integrin–containing focal contacts, as well as reorganization of thicker peripheral F-actin fibrils. Pro-Hyp–mediated chemotactic activity is significantly reduced (p < 0.001) in cells treated with the mitogen-activated protein kinase kinase 1/2 inhibitor PD98059 or the α5β1-integrin antagonist ATN-161. Furthermore, ATN-161 significantly inhibits uptake of Pro-Hyp into adult tenocytes. Thus, our findings document the molecular basis of the functional benefits of the Pro-Hyp dipeptide in cellular behavior. These dynamic properties of collagen-derived Pro-Hyp dipeptide could lead the way to its application in translational medicine. 相似文献
994.
995.
996.
Sohn Joon Hyuk Fukui Dai Nojiri Taro Minowa Kazuhiro Kimura Junpei Koyabu Daisuke 《Journal of Mammalian Evolution》2021,28(2):559-571
Journal of Mammalian Evolution - Anatomy of bat genital organs has been conventionally studied by gross and microscopic observations to date. Here, we employ both histological observation and... 相似文献
997.
Motosugi Nanane Nakamura Futoshi Nakajima Souta Takahata Chihiro Kawamura Kazuhiro Morimoto Junko 《Landscape and Ecological Engineering》2021,17(2):107-107
Landscape and Ecological Engineering - In the original publication of the article, the following reference was not included and provided in this correction. 相似文献
998.
Shirai Y Morioka S Sakuma M Yoshino K Otsuji C Sakai N Kashiwagi K Chida K Shirakawa R Horiuchi H Nishigori C Ueyama T Saito N 《Molecular biology of the cell》2011,22(8):1340-1352
During differentiation, keratinocytes undergo a dramatic shape change from small and round to large and flat, in addition to production of proteins necessary for the formation of epidermis. It has been shown that protein kinase C (PKC) η is crucial for keratinocyte differentiation. However, its role in this process has yet to be fully elucidated. Here, we show that catalytic activity is not necessary for enlarged and flattened morphology of human keratinocytes induced by overexpression of PKCη, although it is important for gene expression of the marker proteins. In addition, we identify the small G protein RalA as a binding partner of PKCη, which binds to the C1 domain, an indispensable region for the morphological change. The binding led activation of RalA and actin depolymerization associated with keratinocyte differentiation. siRNA techniques proved that RalA is involved in not only the keratinocyte differentiation induced by PKCη overexpression but also normal keratinocyte differentiation induced by calcium and cholesterol sulfate. These results provide a new insight into the molecular mechanism of cytoskeletal regulation leading to drastic change of cell shape. 相似文献
999.
Hagiwara M Maegawa K Suzuki M Ushioda R Araki K Matsumoto Y Hoseki J Nagata K Inaba K 《Molecular cell》2011,41(4):432-444
ER-associated degradation (ERAD) is an ER quality-control process that eliminates terminally misfolded proteins. ERdj5 was recently discovered to be a key ER-resident PDI family member protein that accelerates ERAD by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. We here solved the crystal structure of full-length ERdj5, thereby revealing that ERdj5 contains the N-terminal J domain and six tandem thioredoxin domains that can be divided into the N- and C-terminal clusters. Our systematic biochemical analyses indicated that two thioredoxin domains that constitute the C-terminal cluster form the highly reducing platform that interacts with EDEM1 and reduces EDEM1-recruited substrates, leading to their facilitated degradation. The pulse-chase experiment further provided direct evidence for the sequential movement of an ERAD substrate from calnexin to the downstream EDEM1-ERdj5 complex, and then to the retrotranslocation channel, probably through BiP. We present a detailed molecular view of how ERdj5 mediates ERAD in concert with EDEM1. 相似文献
1000.