全文获取类型
收费全文 | 12005篇 |
免费 | 622篇 |
国内免费 | 11篇 |
出版年
2022年 | 66篇 |
2021年 | 133篇 |
2020年 | 62篇 |
2019年 | 94篇 |
2018年 | 137篇 |
2017年 | 123篇 |
2016年 | 186篇 |
2015年 | 300篇 |
2014年 | 351篇 |
2013年 | 723篇 |
2012年 | 619篇 |
2011年 | 556篇 |
2010年 | 345篇 |
2009年 | 352篇 |
2008年 | 598篇 |
2007年 | 568篇 |
2006年 | 564篇 |
2005年 | 539篇 |
2004年 | 545篇 |
2003年 | 546篇 |
2002年 | 496篇 |
2001年 | 433篇 |
2000年 | 429篇 |
1999年 | 354篇 |
1998年 | 172篇 |
1997年 | 131篇 |
1996年 | 104篇 |
1995年 | 100篇 |
1994年 | 87篇 |
1993年 | 97篇 |
1992年 | 261篇 |
1991年 | 258篇 |
1990年 | 207篇 |
1989年 | 182篇 |
1988年 | 189篇 |
1987年 | 165篇 |
1986年 | 155篇 |
1985年 | 152篇 |
1984年 | 131篇 |
1983年 | 119篇 |
1982年 | 95篇 |
1981年 | 75篇 |
1980年 | 66篇 |
1979年 | 98篇 |
1978年 | 90篇 |
1977年 | 67篇 |
1976年 | 61篇 |
1975年 | 53篇 |
1973年 | 61篇 |
1970年 | 49篇 |
排序方式: 共有10000条查询结果,搜索用时 17 毫秒
161.
Two-dimensional gel studies of genetic variation in the plasma proteins of Amerindians and Japanese 总被引:7,自引:0,他引:7
Jun-ichi Asakawa Norio Takahashi Barnett B. Rosenblum James V. Neel 《Human genetics》1985,70(3):222-230
Summary Genetic variation has been studied in plasma samples from 107 Amerindian children and their parents, and 110 Japanese children and their parents by means of two-dimensional polyacrylamide gel electrophoresis. Twenty-three polypeptides were scored; the identity of nine of these is at present still unknown. Genetic variation was encountered in 11 of these polypeptides. We have previously reported that the index of heterozygosity was 6.2±0.7% for 20 randomly selected, silver stained polypeptides scored for genetic variation in Caucasoids (Rosenblum et al. 1983b). For technical reasons only 11 of these 20 polypeptides could be routinely scored in preparations from the Amerindian samples. For these 11 polypeptides, the indices of heterozygosity in the three populations were: Amerindians, 4.5±0.6%; Japanese, 5.7±0.7%; Caucasoids, 8.0±1.1%. Even with these relatively small numbers some striking ethnic differences as regards individual polypeptides are apparent. 相似文献
162.
Characterization of a Mr = 56,000 polypeptide associated with 10S DNA polymerase alpha purified from calf thymus using monoclonal antibody. 总被引:1,自引:0,他引:1 下载免费PDF全文
S Masaki K Tamai R Suzuki K Tanabe T Takahashi S Yoshida 《Nucleic acids research》1985,13(18):6635-6649
Existence of a Mr = 56,000 polypeptide associated with 10S DNA polymerase alpha was shown by production of a monoclonal anti-calf thymus 10S DNA polymerase alpha antibody secreted from a hybridoma line named 3H1. The antibody bound three polypeptides with Mr = 180,000, 56,000 and 32,000 in hydroxylapatite fraction of 10S DNA polymerase alpha by immunoblot. The antibody co-precipitated the polypeptides with the large polypeptide (Mr = 150,000-140,000) of 10S DNA polymerase alpha with the aid of second antibody. Among three polypeptides, the Mr = 56,000 polypeptide was co-purified with DNA polymerase alpha through DNA-cellulose chromatography and repeated sucrose rate-zonal centrifugations. The Mr = 56,000 polypeptide was still associated with 10S DNA polymerase alpha after second sucrose rate-zonal centrifugation, but the amount of it was reduced. The polypeptide was banded at pH 7.2-8.0 and displayed microheterogeneity in respect of isoelectric point by isoelectrofocusing with 7 M urea, and showed weak DNA-binding property after blotting onto a nitrocellulose. The antibody against the polypeptide precipitated DNA polymerase alpha from human, rat, and mouse, and Mr = 56,000 and 32,000 polypeptides were detected in these DNA polymerase alpha fractions by immunoblot. These results suggest that the polypeptide with Mr = 56,000 may take part in the DNA polymerase reaction. 相似文献
163.
Reverse effect of gram-positive bacteria vs. gram-negative bacteria on adjuvant-induced arthritis in germfree rats 总被引:5,自引:0,他引:5
O Kohashi Y Kohashi T Takahashi A Ozawa N Shigematsu 《Microbiology and immunology》1985,29(6):487-497
Germfree (GF) F344 rats developed severe adjuvant-induced arthritis with a 100% incidence after a single intradermal injection of heat-killed Mycobacterium bovis (BCG). Specific pathogene-free (SPF) rats developed less severe arthritis with a lower incidence. The rats colonized with Escherichia coli or Bacteroides developed mild disease comparable to that in SPF rats. The rats colonized with Bifidobacterium, Propionibacterium acnes, Lactobacillus casei, L. fermentum, L. murini, and L. acidophilus developed more severe disease than that in GF rats. Furthermore, the rats colonized with a mixture of E. coli and the above lactobacilli developed very mild disease similar to that in SPF rats. These results suggest that gram-negative bacteria, such as E. coli and Bacteroides, may suppress the disease, possibly through their lipopolysaccharides, and may be responsible for the lower susceptibility of SPF rats; gram-positive bacteria, such as Bifidobacterium, P. acnes, and lactobacilli, may enhance the disease, possibly through their peptidoglycans; and E. coli may play a dominant role in modulating the development of adjuvant-induced arthritis. 相似文献
164.
Purification of cell envelopes of Rickettsia tsutsugamushi 总被引:1,自引:0,他引:1
165.
Selective modification of myosin SH1 with 1,2,4-trinitrobenzene. VIII. Thiols of myosin 总被引:1,自引:0,他引:1
Myosin has 2 mol of the most reactive thiol, named SH1. 1,2,4-Trinitrobenzene (TNB), a novel dinitrophenyl(DNP)ating reagent [Takahashi et al. (1983) Chem. Lett. 1445-1448], was found to react only with SH1 without any other amino acid residues in myosin under the conditions used. Its reaction with myosin SH1 was about 30 times faster than that with N-acetylcysteine (NAC). The reaction rate of TNB with SH1 was about twice compared with that of NEM, the most reactive selective reagent for SH1 so far found, although its rate with NAC was only one sixtieth that of NEM. As to the lambda max of the absorption spectrum of SH1-DNP-myosin, a large red shift of as much as 20 nm was observed compared with low molecular S-DNP derivatives. This red shift disappeared in 8 M urea. This outstanding feature of SH1 modification with TNB was discussed in terms of affinity labeling by interaction with an aromatic amino acid near SH1. 相似文献
166.
Conservation of molecular structure of DNA polymerase beta in vertebrates probed by tryptic peptide mapping 总被引:3,自引:0,他引:3
K Tanabe T Yamaguchi M Saneyoshi M Yamaguchi A Matsukage T Takahashi 《Journal of biochemistry》1984,96(2):365-370
DNA polymerase beta's from mouse myeloma, chick embryo, and cherry salmon testis were all composed of a single polypeptide of about 40K daltons as judged by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis of extensively purified enzyme preparations. Although the enzyme from bullfrog ovary was not fully purified, its molecular weight was estimated to be the same as that of the chick enzyme by immunological detection after electrophoresis. All the enzymes tested cross-reacted immunologically with the antibody against chick DNA polymerase beta, indicating that they have a common molecular structure, at least in part. Two-dimensional maps of radioiodinated tryptic peptides directly showed the presence of highly conserved amino acid sequences among mouse, chick, and cherry salmon enzymes. This conserved structure is thought to be essential for the enzyme activity, which is very similar among all these vertebrates. 相似文献
167.
Purification and characterization of a calcium-activated neutral protease from monkey brain and its action on neuropeptides 总被引:3,自引:0,他引:3
A calcium-activated neutral protease was purified from Japanese monkey brain by ammonium sulfate fractionation and sequential column chromatographies monitored by assay of caseinolytic activity. The purified enzyme gave a single protein band on non-denaturing polyacrylamide gel electrophoresis, and consisted of two subunits with molecular weights of 74,000 and 20,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme required millimolar order calcium ions for activation, and was optimally active at pH 7.5-8.0. Upon incubation with various neuropeptides as substrates, the enzyme preferentially cleaved the peptide bonds with Arg, Lys, or Tyr at the P1 position and an amino acid residue with a bulky aliphatic side chain, such as Leu, Val, or Ile, at the P2 position. The hydrolytic activity toward neuropeptides as well as casein was strongly inhibited by various thiol protease inhibitors. These results suggested that the brain calcium-activated neutral protease may participate in the degradation of neuropeptides in vivo. 相似文献
168.
S100a0 (αα) Protein Is Present in Neurons of the Central and Peripheral Nervous System 总被引:2,自引:1,他引:1
The cellular distribution of S100 subunits in human brain and peripheral nerves was studied by means of an immunohistochemical technique using antibodies specific to the alpha subunit or the beta subunit of S100 protein. The results indicate that the distribution of the alpha subunit and the beta subunit is different among cell types in the nervous tissue, and that neurons in the brain and peripheral nerves contain only the alpha subunit, or S100a0 protein. The subunit distribution also appears to be different at an intracellular level, where the immunoreaction products for the alpha subunit show granular arrangement whereas those for the beta subunit are found diffusely in the cytoplasm. 相似文献
169.
170.
Yutaka Tamaura Katsunobu Takahashi Yoh Kodera Yuji Saito Yuji Inada 《Biotechnology letters》1986,8(12):877-880
Summary A ferromagnetic modifier was prepared by reacting ferrous(Fe2+)- and ferric(Fe3+)-ions with polyethylene glycol having two carboxyl groups (MW:2000) at pH 8.0–8.5. Lipase fromPseudomonas
fragi 22–39B was coupled with the modifier using water-soluble carbodiimide. The modified lipase, which was dispersed into buffered solutions in the size range of 30–70 nm, exerted the hydrolytic activity of 8.0 U/mg. In a magnetic field of 250 Oe, the ferromagnetic-modified lipase was readily recovered from the colloidal solution. 相似文献