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排序方式: 共有470条查询结果,搜索用时 15 毫秒
461.
Jumpei Uchiyama Mohammad Rashel Yoshihiro Maeda Iyo Takemura Shigeyoshi Sugihara Kazue Akechi Asako Muraoka Hiroshi Wakiguchi Shigenobu Matsuzaki 《FEMS microbiology letters》2008,278(2):200-206
Vancomycin-resistant Enterococcus faecalis (VRE) has become a significant threat in nosocomial settings. Bacteriophage (phage) therapy is frequently proposed as a potential alternative therapy for infections caused by this bacterium. To search for candidate therapeutic phages against Enterococcus faecalis infections, 30 Enterococcus faecalis phages were isolated from the environment. One of these, virulent phage phiEF24C, which has a broad host range, was selected for analysis. The plaque-forming ability of phiEF24C was virtually unaffected by differences in the clinical host strains. Furthermore, the phage had a shorter latent period and a larger burst size than ordinary tailed phages, indicating that phiEF24C has effective lytic activity against many Enterococcus faecalis strains, including VRE. Morphological and genomic analyses revealed that phiEF24C is a large myovirus (classified as family Myoviridae morphotype A1) with a linear double-stranded DNA genome of c. 143 kbp. Analyses of the N-terminal amino acid sequences of the virion proteins, together with the morphology and the genome size, speculated that phiEF24C is closely related to other myoviruses of Gram-positive bacteria that have been used experimentally or practically for therapy or prophylaxis. Considering these results, phiEF24C may be a potential candidate therapeutic phage against Enterococcus faecalis infections. 相似文献
462.
Shoji Tsunekawa Katsuhito Takahashi Masahiro Abe Kunio Hiwada Kazue Ozawa Takashi Murachi 《FEBS letters》1989,250(2):493-496
Calponin, a novel homologue of troponin T, purified from chicken gizzard was found to be one of the most susceptible proteins among smooth muscle contraction-associated proteins to hydrolysis by calpain I purified from human red blood cells. The high susceptibility of calponin was comparable to that reported for troponin T. The rate of degradation of calponin, unlike caldesmon and myosin light chain kinase, was accelerated when bound to calmodulin. When calponin existed as a bound form in both reconstituted actin filament and native thin filament, the rate of proteolysis was markedly retarded, indicating close association of calponin with actin filament. These observations are compatible with the view that calponin is an integral part of the actin-linked contractile machinery in smooth muscle. 相似文献
463.
Kazue Tabita Sumiko Sakaguchi Shunji Kozaki Genji Sakaguchi 《FEMS microbiology letters》1991,79(2-3):251-256
In Bordetella pertussis virulence-associated genes, including adenylate cyclase toxin (Cya), are coordinately regulated in response to environmental signals by proteins coded by the bvg-locus. We have constructed cya-lac fusions in Escherichia coli and have shown that the cya operon is not expressed in E. coli, neither is it activated by bvg, when introduced in trans. The cya-lac fusion is fully active when returned to B. pertussis by homologous recombination and responds to bvg-dependent activation and environmental regulation. These results indicate that in B. pertussis the activation of the cya operon by bvg is indirect. 相似文献
464.
Shiro Someya Kenjiro Koyama Nozomu Asami Tetsuo Kataoka Yuichi Yamamura 《Microbiology and immunology》1966,10(4):221-227
A comparative study of the tuberculin potency and reactivity to TAP and PPD-S was made in humans and guinea pigs. A comparison of reactions elicited by TAP and PPD-S was made in two groups of guinea pigs previously inoculated with killed bacilli or with living bacilli. The tuberculin reactions produced by TAP and PPD-S in human beings was studied in school children from rural districts. These children were divided randomly into three groups and each group was given a different amount of TAP and PPD-S. The results obtained are as follows: (1) While relative potency of TAP was relatively much weaker than PPD-S in guinea pigs sensitized with tubercle bacilli, in human beings the tuberculin potency of TAP was approximately 1/2 or 1/3 that of PPD-S. (2) There was a marked difference in the tuberculin reactivity to TAP and PPD-S between guinea pigs immunized with killed bacilli and guinea pigs immunized with living bacilli. 相似文献
465.
A Someya T Akiyama M Misumi N Tanaka 《Biochemical and biophysical research communications》1978,85(4):1542-1550
For the purpose of elucidating the biochemical mechanism of anthracycline cardiomyopathy, the interaction with actin and heavy meromyosin (HMM) was studied. HMM and acto-HMM Mg2+-ATPase reactions were inhibited by daunorubicin and adriamycin; but not significantly by aclacinomycin A. The three antibiotics induced G-actin polymerization. Difference absorption spectra showed a direct interaction of adriamycin or aclacinomycin A with actin or HMM. Equilibrium dialysis and spectrofluorometric studies indicated that actin monomer possesses one binding site for adriamycin or aclacinomycin A with the same order of association constants (1.4 – 7.2 × 104 M?1). Adriamycin exhibited significantly higher affinity for HMM than aclacinomycin A. 相似文献
466.
Time-lapse monitoring using infrared imaging revealed a distinct change in circumnutatory behaviour of Arabidopsis inflorescence stems by dark treatment, which drastically increased curvature and decreased nutation frequency. Re-irradiation
during dark treatment had different effect on the nutation frequency and the curvature, suggesting that radiation condition
controls them through different mechanism. 相似文献
467.
Velandia Vargas Jorge Enrique Falco Daniela Godoy da Silva Walter Arnaldo César Cavaliero Carla Kazue Nakao Seabra Joaquim Eugênio Abel 《The International Journal of Life Cycle Assessment》2019,24(10):1878-1897
The International Journal of Life Cycle Assessment - Environmental impacts of battery electric vehicles (BEVs) and internal combustion engine vehicles have been broadly studied and compared.... 相似文献
468.
Jumpei Uchiyama Mohammad Rashel Yoshihiro Maeda Iyo Takemura Shigeyoshi Sugihara Kazue Akechi Asako Muraoka Hiroshi Wakiguchi & Shigenobu Matsuzaki 《FEMS microbiology letters》2008,278(2):200-206
Vancomycin-resistant Enterococcus faecalis (VRE) has become a significant threat in nosocomial settings. Bacteriophage (phage) therapy is frequently proposed as a potential alternative therapy for infections caused by this bacterium. To search for candidate therapeutic phages against Enterococcus faecalis infections, 30 Enterococcus faecalis phages were isolated from the environment. One of these, virulent phage φEF24C, which has a broad host range, was selected for analysis. The plaque-forming ability of φEF24C was virtually unaffected by differences in the clinical host strains. Furthermore, the phage had a shorter latent period and a larger burst size than ordinary tailed phages, indicating that φEF24C has effective lytic activity against many Enterococcus faecalis strains, including VRE. Morphological and genomic analyses revealed that φEF24C is a large myovirus (classified as family Myoviridae morphotype A1) with a linear double-stranded DNA genome of c . 143 kbp. Analyses of the N-terminal amino acid sequences of the virion proteins, together with the morphology and the genome size, speculated that φEF24C is closely related to other myoviruses of Gram-positive bacteria that have been used experimentally or practically for therapy or prophylaxis. Considering these results, φEF24C may be a potential candidate therapeutic phage against Enterococcus faecalis infections. 相似文献
469.
470.
Kazuo Honda Kanji Ishizaki Mituo Ikenaga Junya Toguchida Takashi Inamoto Kouichi Tanaka Kazue Ozawa 《Human genetics》1988,79(4):297-300
Summary We have examined the c-Ha-ras locus in 145 cancer patients of a mixed group and 164 normal individuals in Japan for restriction fragment length polymorphisms and compared the allele distributions in normal and cancer populations. The c-Ha-ras gene is highly polymorphic in Japanese as previously reported in Caucasians. Two rare alleles were found to be present with increased frequencies in Japanese cancer patients. These results suggest that genotype analysis of the c-Ha-ras gene could be used to detect cancer-prone individuals. 相似文献