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241.
Naoshi Ohta Masato Sato Kiminori Ushida Mami Kokubo Takayuki Baba Kayoko Taniguchi Makoto Urai Koji Kihira Joji Mochida 《BMC biotechnology》2009,9(1):1-11
Background
Enzyme production in microbial cells has been limited to secreted enzymes or intracellular enzymes followed by expensive down stream processing. Extracellular enzymes consists mainly of hydrolases while intracellular enzymes exhibit a much broader diversity. If these intracellular enzymes could be secreted by the cell the potential of industrial applications of enzymes would be enlarged. Therefore a novel secretion pathway for intracellular proteins was developed, using peroxisomes as secretion vesicles.Results
Peroxisomes were decorated with a Golgi derived v-SNARE using a peroxisomal membrane protein as an anchor. This allowed the peroxisomes to fuse with the plasma membrane. Intracellular proteins were transported into the peroxisomes by adding a peroxisomal import signal (SKL tag). The proteins which were imported in the peroxisomes, were released into the extra-cellular space through this artificial secretion pathway which was designated peroxicretion. This concept was supported by electron microscopy studies.Conclusion
Our results demonstrate that it is possible to reroute the intracellular trafficking of vesicles by changing the localisation of SNARE molecules, this approach can be used in in vivo biological studies to clarify the different control mechanisms regulating intracellular membrane trafficking. In addition we demonstrate peroxicretion of a diverse set of intracellular proteins. Therefore, we anticipate that the concept of peroxicretion may revolutionize the production of intracellular proteins from fungi and other microbial cells, as well as from mammalian cells. 相似文献242.
The p38 mitogen-activated protein (MAP) kinase is the central signaling molecule regulating the cellular response to a multitude of external stimuli. Thus, inhibitors of this enzyme are postulated to have significant therapeutic potential for the treatment of some diseases, especially where aberrant cytokine signaling is the driver of disease. Here we established a simple inhibitor screening method for a human protein by using bacteria in combination with the growth recovery as an index. The screening successfully identified benzyl coumarin derivatives as p38 inhibitors. These compounds not only rescue growth retardation of p38-transformed bacteria but also inhibit p38 activity in vitro and in human cells. This study demonstrates that this is a promising and economical inhibitor screening method not only for p38 but also for other proteins. 相似文献
243.
Takada K Hayashi K Sasaki K Sato T Hirasawa M 《Journal of microbiological methods》2006,66(3):460-465
An evaluation on the applicability of Mitis Salivarius agar (MS) medium, commonly used for the detection of oral streptococci in human and animals, to dog specimens and the development of a new selective medium for isolating streptococci from the canine oral cavity are described. Oral samples from dogs were cultured on MS medium under anaerobic conditions. The predominant facultative anaerobic bacteria on MS plates were gram-negative rods. Selectivity of streptococci on MS medium was 21.2%. A new selective medium, designated MS-CAN-AE, was developed for the isolation of streptococci from the canine oral cavity. The average growth recovery of laboratory and clinically isolated strains of streptococci on MS-CAN-AE medium was 84.1% of that on MS medium. Gram-positive rods and gram-negative rods and cocci rarely grew on the MS-CAN-AE. The selectivity of MS-CAN-AE was 95.0% for clinical samples. MS-CAN-AE medium will be helpful for investigations of streptococci in the canine oral cavity. 相似文献
244.
Pattern of natural 15N abundance in lakeside forest ecosystem affected by cormorant-derived nitrogen
Kayoko Kameda Keisuke Koba Satoru Hobara Takashi Osono Masakazu Terai 《Hydrobiologia》2006,567(1):69-86
Waterbirds are one of the most important groups of organisms inhabiting the land–water interface, especially with regard to
mediating the transport of materials from the aquatic to the terrestrial environment. The great cormorant (Phalacrocorax carbo) is a colonial piscivorous bird that transports nutrients from fresh water to forest. We measured cormorant-derived nitrogen
at two nesting colonies on the Isaki Peninsula and Chikubu Island at Lake Biwa, Japan, and analyzed the long-term effects
of cormorant colonization on the forest nitrogen cycle, and the mechanisms of nitrogen retention. Three sites were examined
in each colony: a currently occupied area, a previously occupied but now abandoned area, and a control area never colonized
by cormorants. High nitrogen stable isotope ratios of cormorant excreta, the forest floor, mineral soil, and living plants
showed cormorant-derived nitrogen in both occupied and abandoned areas. The relationship between δ15N and N content showed that the high δ15N of the excreta and N turnover in the soil were important at the occupied sites, whereas high δ15N of litter was important at the abandoned sites. Physiological changes of various organisms are also important for the N
decomposition process. In conclusion, cormorant-derived nitrogen remains in the forest ecosystem as a result of two cormorant
activities: heavy deposition of excreta and collection of nitrogen-rich nest material. Colony stage (occupied, abandoned,
or never inhabited) and historical change of N decomposition process of an area can be identified from the relationship between
δ15N and N content. 相似文献
245.
246.
Asami J Odani H Ishii A Oide K Sudo T Nakamura A Miyata N Otsuka N Maeda K Nakagawa J 《Bioscience, biotechnology, and biochemistry》2006,70(12):2899-2905
Unilateral ureteral obstruction (UUO) of kidneys causes acute generation of carbonyl stress. By electrospray ionization/liquid chromatography/mass spectrometry (ESI/LC/MS) we measured the content of methyl glyoxal, glyoxal, and 3-deoxyglucosone in mouse kidney extracts following UUO. UUO resulted in elevation of these dicarbonyls in the obstructed kidneys. Furthermore, the accumulation of 3-deoxyglucosone was significantly reduced in the kidneys of mice transgenic for alpha-dicarbonyl/L-xylulose reductase (DCXR) as compared to their wild-type littermates, demonstrating 4.91+/-2.04 vs. 6.45+/-1.85 ng/mg protein (P=0.044) for the obstructed kidneys, and 3.68+/-1.95 vs. 5.20+/-1.39 ng/mg protein (P=0.026) for the contralateral kidneys. On the other hand, collagen III content in kidneys showed no difference as monitored by in situ hybridization. Collectively, DCXR may function in the removal of renal alpha-dicarbonyl compounds under oxidative circumstances, but it was not sufficient to suppress acute renal fibrosis during 7 d of UUO by itself. 相似文献
247.
Cao Z Jensen LD Rouhi P Hosaka K Länne T Steffensen JF Wahlberg E Cao Y 《Nature protocols》2010,5(12):1903-1910
Hypoxia-induced vascular responses, including angiogenesis, vascular remodeling and vascular leakage, significantly contribute to the onset, development and progression of retinopathy. However, until recently there were no appropriate animal disease models recapitulating adult retinopathy available. In this article, we describe protocols that create hypoxia-induced retinopathy in adult zebrafish. Adult fli1:EGFP zebrafish are placed in hypoxic water for 3-10 d and retinal neovascularization is analyzed using confocal microscopy. It usually takes 11 d to obtain conclusive results using the hypoxia-induced retinopathy model in adult zebrafish. This model provides a unique opportunity to study kinetically the development of retinopathy in adult animals using noninvasive protocols and to assess therapeutic efficacy of orally active antiangiogenic drugs. 相似文献
248.
Yasukiyo Yoshioka Yue Qi Ye Kiyoshi Okada Kayoko Taniguchi Ayaka Yoshida Kouichi Sugaya Jun-ichi Onose Hiroyuki Koshino Shunya Takahashi Arata Yajima Shunsuke Yajima Naoki Abe 《PloS one》2013,8(12)
Tumor necrosis factor alpha (TNF-α), a central mediator of the inflammatory response, is released from basophilic cells and other cells in response to a variety of proinflammatory stimuli. Vialinin A is a potent inhibitor of TNF-α production and is released from RBL-2H3 cells. Ubiquitin-specific peptidase 5 (USP5), a deubiquitinating enzyme, was identified as a target molecule of vialinin A and its enzymatic activity was inhibited by vialinin A. Here we report production of TNF-α is decreased in USP5 siRNA-knockdown RBL-2H3 cells, compared with control cells. The finding of the present study strongly suggests that USP5 is one of the essential molecules for the production of TNF-α in RBL-2H3. 相似文献
249.
Okuyama K Nishiura C Mizushima F Minoura K Sumida M Taniguchi T Tomoo K Ishida T 《The FEBS journal》2008,275(7):1529-1539
Although one of the priorities in Alzheimer's research is to clarify the filament formation mechanism for the tau protein, it is still unclear how it is transformed from a normal structure in a neuron. To examine the linkage-dependent contribution of each repeat peptide (R1-R4) to filament formation of the three- or four-repeat microtubule-binding domain (MBD) in the tau protein, four two-repeat peptides (R12, R13, R23 and R34) and two three-repeat peptides (R123 and R234) were prepared, and their in vitro self-aggregation was investigated by thioflavin S fluorescence and circular dichroism measurements, and by electron microscopy in neutral buffer (pH 7.6). Comparison of these aggregation behaviors with previous results for single-repeat peptides and wild-type 3RMBD (R134) and 4RMBD (R1234) indicated that (a) the two-repeat R23, not the R2 or R3 single repeat, forms the core structure in self-aggregation of 4RMBD, whereas that of 3RMBD comprises the R3 single repeat, (b) co-existence of R1 and R4 repeats is necessary for the aggregation behavior inherent in 3RMBD and 4RMBD, whereas the R1 or R4 repeat alone functions as a repressor or modifier of the filament formation, (c) 4RMBD aggregation is accompanied by R1-driven transition from random and alpha-helix structures to a beta-sheet structure, whereas 3RMBD aggregation involves three-repeat R134-specific transition from a random structure to an alpha-helix structure without the participation of a beta-sheet structure, and (d) the peptides that include the R1 repeat form a long filament irrespective of the absence or presence of the R4 repeat, whereas those that include the R4 repeat, but not the R1 repeat, form a relatively short filament. To the best of our knowledge, a systematic study of the linkage-dependent contribution of each repeat peptide to the paired helical filament formation of tau MBD has not been carried out previously, and thus the present information is useful for understanding the essence of the filament formation of tau MBD. 相似文献
250.
Kanae Gamo Hiroyuki Miyachi Kayoko Nakamura 《Bioscience, biotechnology, and biochemistry》2013,77(6):1052-1059
In previous reports, hesperidin, a flavonoid glucoside from citrus fruit, is hydrolyzed to hesperetin, an aglycone of hesperidin, and converted to the hesperetin glucuronides (H7-OG and H3′-OG) in vivo and depresses blood glucose levels. But there are no reports on the activity of hesperetin glucuronides. To determine the activity of hesperetin glucuronides, H7-OG and H3′-OG were synthesized and peroxisome proliferator-activated receptor-γ (PPARγ) agonist activity was observed at 250?μM. These glucuronides accelerated the differentiation of 3T3-L1 cells into adipocytes at 10?μM. Furthermore, H7-OG showed additive effects in reporter gene assays and caused noncompetitive reactions in time-resolved fluorescence resonance energy transfer assays with a thiazolidinedione derivative. Our results indicated that hesperetin glucuronides activated PPARγ, accelerated adipocyte differentiation. 相似文献