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91.
D. Pimentel S. Cooperstein H. Randell D. Filiberto S. Sorrentino B. Kaye C. Nicklin J. Yagi J. Brian J. O’Hern A. Habas C. Weinstein 《Human ecology: an interdisciplinary journal》2007,35(6):653-668
The World Health Organization (WHO) and other organizations report that the prevalence of human diseases during the past decade
is rapidly increasing. Population growth and the pollution of water, air, and soil are contributing to the increasing number
of human diseases worldwide. Currently an estimated 40% of world deaths are due to environmental degradation. The ecology
of increasing diseases has complex factors of environmental degradation, population growth, and the current malnutrition of
about 3.7 billion people in the world. 相似文献
92.
Classification and prediction of clinical Alzheimer's diagnosis based on plasma signaling proteins 总被引:10,自引:0,他引:10
Ray S Britschgi M Herbert C Takeda-Uchimura Y Boxer A Blennow K Friedman LF Galasko DR Jutel M Karydas A Kaye JA Leszek J Miller BL Minthon L Quinn JF Rabinovici GD Robinson WH Sabbagh MN So YT Sparks DL Tabaton M Tinklenberg J Yesavage JA Tibshirani R Wyss-Coray T 《Nature medicine》2007,13(11):1359-1362
A molecular test for Alzheimer's disease could lead to better treatment and therapies. We found 18 signaling proteins in blood plasma that can be used to classify blinded samples from Alzheimer's and control subjects with close to 90% accuracy and to identify patients who had mild cognitive impairment that progressed to Alzheimer's disease 2-6 years later. Biological analysis of the 18 proteins points to systemic dysregulation of hematopoiesis, immune responses, apoptosis and neuronal support in presymptomatic Alzheimer's disease. 相似文献
93.
Model-based, three-dimensional (3D) image reconstruction procedures require a starting model to initiate data analysis. We have designed an ab initio method, which we call the random model (RM) method, that automatically generates models to initiate structural analysis of icosahedral viruses imaged by cryo-electron microscopy. The robustness of the RM procedure was demonstrated on experimental sets of images for five representative viruses. The RM method also provides a straightforward way to generate unbiased starting models to derive independent 3D reconstructions and obtain a more reliable assessment of resolution. The fundamental scheme embodied in the RM method should be relatively easy to integrate into other icosahedral software packages. 相似文献
94.
95.
Jong-Min Lee Kyung-Hee Kim Aram Shin Michael?J. Chao Kawther Abu?Elneel Tammy Gillis Jayalakshmi?Srinidhi Mysore Julia?A. Kaye Hengameh Zahed Ian?H. Kratter Aaron?C. Daub Steven Finkbeiner Hong Li Jared?C. Roach Nathan Goodman Leroy Hood Richard?H. Myers Marcy?E. MacDonald James?F. Gusella 《American journal of human genetics》2015,97(3):435-444
Huntington disease (HD) reflects the dominant consequences of a CAG-repeat expansion in HTT. Analysis of common SNP-based haplotypes has revealed that most European HD subjects have distinguishable HTT haplotypes on their normal and disease chromosomes and that ∼50% of the latter share the same major HD haplotype. We reasoned that sequence-level investigation of this founder haplotype could provide significant insights into the history of HD and valuable information for gene-targeting approaches. Consequently, we performed whole-genome sequencing of HD and control subjects from four independent families in whom the major European HD haplotype segregates with the disease. Analysis of the full-sequence-based HTT haplotype indicated that these four families share a common ancestor sufficiently distant to have permitted the accumulation of family-specific variants. Confirmation of new CAG-expansion mutations on this haplotype suggests that unlike most founders of human disease, the common ancestor of HD-affected families with the major haplotype most likely did not have HD. Further, availability of the full sequence data validated the use of SNP imputation to predict the optimal variants for capturing heterozygosity in personalized allele-specific gene-silencing approaches. As few as ten SNPs are capable of revealing heterozygosity in more than 97% of European HD subjects. Extension of allele-specific silencing strategies to the few remaining homozygous individuals is likely to be achievable through additional known SNPs and discovery of private variants by complete sequencing of HTT. These data suggest that the current development of gene-based targeting for HD could be extended to personalized allele-specific approaches in essentially all HD individuals of European ancestry. 相似文献
96.
Shijun Li Min Tan Franceline Juillard Rajesh Ponnusamy Bruno Correia J. Pedro Simas Maria A. Carrondo Colin E. McVey Kenneth M. Kaye 《The Journal of biological chemistry》2015,290(47):28084-28096
Kaposi sarcoma-associated herpesvirus (KSHV) has a causative role in several human malignancies. KSHV latency-associated nuclear antigen (LANA) mediates persistence of viral episomes in latently infected cells. LANA mediates KSHV DNA replication and segregates episomes to progeny nuclei. The structure of the LANA DNA binding domain was recently solved, revealing a positive electrostatic patch opposite the DNA binding surface, which is the site of BET protein binding. Here we investigate the functional role of the positive patch in LANA-mediated episome persistence. As expected, LANA mutants with alanine or glutamate substitutions in the central, peripheral, or lateral portions of the positive patch maintained the ability to bind DNA by EMSA. However, all of the substitution mutants were deficient for LANA DNA replication and episome maintenance. Mutation of the peripheral region generated the largest deficiencies. Despite these deficiencies, all positive patch mutants concentrated to dots along mitotic chromosomes in cells containing episomes, similar to LANA. The central and peripheral mutants, but not the lateral mutants, were reduced for BET protein interaction as assessed by co-immunoprecipitation. However, defects in BET protein binding were independent of episome maintenance function. Overall, the reductions in episome maintenance closely correlated with DNA replication deficiencies, suggesting that the replication defects account for the reduced episome persistence. Therefore, the electrostatic patch exerts a key role in LANA-mediated DNA replication and episome persistence and may act through a host cell partner(s) other than a BET protein or by inducing specific structures or complexes. 相似文献
97.
An assessment of the microbiological safety of fresh whole‐leaf herbs from retail premises in the United Kingdom with a focus on Salmonella spp. 下载免费PDF全文
98.
Crone EE Menges ES Ellis MM Bell T Bierzychudek P Ehrlén J Kaye TN Knight TM Lesica P Morris WF Oostermeijer G Quintana-Ascencio PF Stanley A Ticktin T Valverde T Williams JL 《Ecology letters》2011,14(1):1-8
Matrix projection models are among the most widely used tools in plant ecology. However, the way in which plant ecologists use and interpret these models differs from the way in which they are presented in the broader academic literature. In contrast to calls from earlier reviews, most studies of plant populations are based on < 5 matrices and present simple metrics such as deterministic population growth rates. However, plant ecologists also cautioned against literal interpretation of model predictions. Although academic studies have emphasized testing quantitative model predictions, such forecasts are not the way in which plant ecologists find matrix models to be most useful. Improving forecasting ability would necessitate increased model complexity and longer studies. Therefore, in addition to longer term studies with better links to environmental drivers, priorities for research include critically evaluating relative/comparative uses of matrix models and asking how we can use many short-term studies to understand long-term population dynamics. 相似文献
99.
Sequences of the novel gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV) have been described in human prostate cancer tissue, although the amounts of DNA are low. Furthermore, XMRV sequences and polytropic (p) murine leukemia viruses (MLVs) have been reported in patients with chronic fatigue syndrome (CFS). In assessing the prevalence of XMRV in prostate cancer tissue samples we discovered that eluates from naïve DNA purification columns, when subjected to PCR with primers designed to detect genomic mouse DNA contamination, occasionally gave rise to amplification products. Further PCR analysis, using primers to detect XMRV, revealed sequences derived from XMRV and pMLVs from mouse and human DNA and DNA of unspecified origin. Thus, DNA purification columns can present problems when used to detect minute amounts of DNA targets by highly sensitive amplification techniques. 相似文献
100.
Perrin RJ Craig-Schapiro R Malone JP Shah AR Gilmore P Davis AE Roe CM Peskind ER Li G Galasko DR Clark CM Quinn JF Kaye JA Morris JC Holtzman DM Townsend RR Fagan AM 《PloS one》2011,6(1):e16032