全文获取类型
收费全文 | 1443篇 |
免费 | 88篇 |
国内免费 | 1篇 |
出版年
2022年 | 7篇 |
2021年 | 13篇 |
2019年 | 15篇 |
2018年 | 21篇 |
2017年 | 14篇 |
2016年 | 24篇 |
2015年 | 32篇 |
2014年 | 42篇 |
2013年 | 55篇 |
2012年 | 80篇 |
2011年 | 75篇 |
2010年 | 44篇 |
2009年 | 46篇 |
2008年 | 51篇 |
2007年 | 82篇 |
2006年 | 73篇 |
2005年 | 63篇 |
2004年 | 54篇 |
2003年 | 51篇 |
2002年 | 67篇 |
2001年 | 63篇 |
2000年 | 55篇 |
1999年 | 56篇 |
1998年 | 23篇 |
1997年 | 18篇 |
1996年 | 19篇 |
1995年 | 17篇 |
1994年 | 12篇 |
1993年 | 16篇 |
1992年 | 31篇 |
1991年 | 15篇 |
1990年 | 25篇 |
1989年 | 32篇 |
1988年 | 27篇 |
1987年 | 17篇 |
1986年 | 22篇 |
1985年 | 11篇 |
1984年 | 11篇 |
1983年 | 10篇 |
1981年 | 15篇 |
1980年 | 12篇 |
1979年 | 12篇 |
1977年 | 11篇 |
1975年 | 9篇 |
1974年 | 9篇 |
1973年 | 11篇 |
1972年 | 11篇 |
1969年 | 9篇 |
1968年 | 8篇 |
1966年 | 6篇 |
排序方式: 共有1532条查询结果,搜索用时 15 毫秒
121.
Kawakami T Nagata T Muraguchi A Nishimura T 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2003,787(1):223-229
Apoptosis is an essential process for selection of T lymphocytes specific for foreign antigen in the process of mammalian thymus maturation. Proteomics, a comprehensive study of proteins expressed in a cell, will facilitate the systematic analysis of protein molecules related to such a complicated biological system. Protein expression profiles including information about protein signatures, localization and their quantitative changes with extracellular stimulations are extremely useful to construct intracellular pathway models resulting in the apoptotic cell death. 相似文献
122.
Excessive increase of serum interleukin 6 jeopardizes host defense against multi-bacterial infection 总被引:1,自引:0,他引:1
Serum interleukin 6 (IL-6) is elevated among patients who have undergone surgery, trauma, and thermal injury. It is well known that the greater the increase of serum IL-6, the higher the incidence of post-injury morbidity and mortality is. However, it has not been determined whether the physiological effects of IL-6 increase the rate of morbidity and mortality or if IL-6 is just a bystander that only indicates the severity of the injury. To elucidate this, we planned to investigate the effect of IL-6 on a multi-bacterial infection, one of the most frequent post-injury complications. CDF1 male mice were administered recombinant human IL-6 (hIL-6) continuously at a dose of 0, 1, or 10 microg/day. The mice then underwent cecal ligation without puncture that induced slow multi-bacterial infection. The survival rate of mice receiving 10 microg/day of hIL-6 was significantly lower (38.5%) than the rate of those receiving 0 (83.3%) or 1 (92.3%) microg/day of hIL-6. The result of this study showed that only excessive increases in serum IL-6, to levels that were observed among patients who underwent severe injury or extensive surgery with high incidence of post-injury infection, jeopardize the host's defense against bacterial infection. 相似文献
123.
NRSF regulates the fetal cardiac gene program and maintains normal cardiac structure and function 总被引:1,自引:0,他引:1
124.
Kajiya A Koyama Y Mita T Mega T Hase S Kawakami T Honda E Munakata H Isemura M 《Bioscience, biotechnology, and biochemistry》2003,67(9):2051-2054
To develop a convenient method to isolate lectins, we prepared an affinity gel by coupling plasma proteins with agarose beads under conditions where the pH did not exceed 7.5. The validity of the use of this affinity gel in combination with elution using a hapten saccharide was confirmed by isolation of concanavalin A from Jack bean meal. Successful application of the method was demonstrated by isolation of two novel vegetable lectins from udo (Aralia cordate) and wasabi (Wasabia japonica). The method would be useful to isolate new lectins from various sources including plant and animal tissues. 相似文献
125.
Kang SW Wahl MI Chu J Kitaura J Kawakami Y Kato RM Tabuchi R Tarakhovsky A Kawakami T Turck CW Witte ON Rawlings DJ 《The EMBO journal》2001,20(20):5692-5702
Mutations in Bruton's tyrosine kinase (Btk) result in X-linked agammaglobulinemia (XLA) in humans and X-linked immunodeficiency (xid) in mice. While targeted disruption of the protein kinase C-beta (PKCbeta) gene in mice results in an immunodeficiency similar to xid, the overall tyrosine phosphorylation of Btk is significantly enhanced in PKCbeta-deficient B cells. We provide direct evidence that PKCbeta acts as a feedback loop inhibitor of Btk activation. Inhibition of PKCbeta results in a dramatic increase in B-cell receptor (BCR)-mediated Ca2+ signaling. We identified a highly conserved PKCbeta serine phosphorylation site in a short linker within the Tec homology domain of Btk. Mutation of this phosphorylation site led to enhanced tyrosine phosphorylation and membrane association of Btk, and augmented BCR and FcepsilonRI-mediated signaling in B and mast cells, respectively. These findings provide a novel mechanism whereby reversible translocation of Btk/Tec kinases regulates the threshold for immunoreceptor signaling and thereby modulates lymphocyte activation. 相似文献
126.
Kawakami T Chiba T Suzuki T Iwai K Yamanaka K Minato N Suzuki H Shimbara N Hidaka Y Osaka F Omata M Tanaka K 《The EMBO journal》2001,20(15):4003-4012
NEDD8/Rub1 is a ubiquitin (Ub)-like post-translational modifier that is covalently linked to cullin (Cul)-family proteins in a manner analogous to ubiquitylation. NEDD8 is known to enhance the ubiquitylating activity of the SCF complex (composed of Skp1, Cul-1, ROC1 and F-box protein), but the mechanistic role is largely unknown. Using an in vitro reconstituted system, we report here that NEDD8 modification of Cul-1 enhances recruitment of Ub-conjugating enzyme Ubc4 (E2) to the SCF complex (E3). This recruitment requires thioester linkage of Ub to Ubc4. Our findings indicate that the NEDD8-modifying system accelerates the formation of the E2-E3 complex, which stimulates protein polyubiquitylation. 相似文献
127.
128.
Honda S Okeda J Iwanaga H Kawakami S Taga A Suzuki S Imai K 《Analytical biochemistry》2000,286(1):99-111
A method for ultramicroanalysis of carbohydrates using capillary electrophoresis with laser-induced fluorescence detection was developed, based on precapillary conversion to 7-nitro-2,1, 3-benzoxadiazole (NBD)-tagged N-methylglycamines. Although the derivatization involves two-step reactions, i.e., reductive N-methylamination followed by condensation with NBD-F, they can be carried out in a one-pot fashion and proceed quantitatively within ca. 50 min in total. Since the reaction conditions are mild, it does not cause desialylation. The derivatives can be well separated by capillary electrophoresis and sensitively detected by argon laser-induced fluorescence. It allowed detection of monosaccharides of down to nanomolar concentrations for analytical sample solution, which correspond to the attomole injected amounts, and good linearity was observed over a wide range. It was also successfully applied to analysis of N-glycans in a microgram quantity of a glycoprotein. Studies on the cleanup of derivatized product are also described in relation to N-glycan analysis. 相似文献
129.
K Kumagai T Abe W F Brechue T Ryushi S Takano M Mizuno 《Journal of applied physiology》2000,88(3):811-816
The purpose of this study was to investigate the relationship between muscle fascicle length and sprint running performance in 37 male 100-m sprinters. The sample was divided into two performance groups by the personal-best 100-m time: 10.00-10.90 s (S10; n = 22) and 11.00-11.70 s (S11; n = 15). Muscle thickness and fascicle pennation angle of the vastus lateralis and gastrocnemius medialis and lateralis muscles were measured by B-mode ultrasonography, and fascicle length was estimated. Standing height, body weight, and leg length were similar between groups. Muscle thickness was similar between groups for vastus lateralis and gastrocnemius medialis, but S10 had a significantly greater gastrocnemius lateralis muscle thickness. S10 also had a greater muscle thickness in the upper portion of the thigh, which, given similar limb lengths, demonstrates an altered "muscle shape." Pennation angle was always less in S10 than in S11. In all muscles, S10 had significantly greater fascicle length than did S11, which significantly correlated with 100-m best performance (r values from -0.40 to -0.57). It is concluded that longer fascicle length is associated with greater sprinting performance. 相似文献
130.