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991.
992.
I Hirao G Kawai K Kobayashi Y Nishimura K Miura K Watanabe Y Ishido 《Nucleic acids symposium series》1992,(27):127-128
The deoxyribonucleotide fragments, d(GCGAAAGC) and d(GCGAAGC) form extraordinarily stable mini-hairpin structures containing only two G-C pairs. In contrast, the corresponding ribonucleotide fragments, r(GCGAAAGC) and r(GCGAAGC) are not so stable, although they also form mini-hairpin structures. The stability difference between these DNA and RNA mini-hairpins was examined by NMR studies and the molecular mechanics calculations, and by comparing with the stability of several sequence variants. Their stability was deduced to vary delicately depending on helical patterns formed by DNA (B form) and RNA (A form) structures. 相似文献
993.
994.
995.
Activation of glycogenolysis in neonatal liver 总被引:2,自引:0,他引:2
996.
The complete amino acid sequence of bovine heart cytochrome C1 总被引:7,自引:0,他引:7
S Wakabayashi H Matsubara C H Kim K Kawai T E King 《Biochemical and biophysical research communications》1980,97(4):1548-1554
997.
M Kawai N Fukuta N Ito T Kagami Y Butsugan M Maruyama Y Kudo 《International journal of peptide and protein research》1990,35(5):452-459
Analogs of opioid pentapeptide [D-Ala2,Leu5]enkephalin were prepared using two kinds of N-methylation reactions, namely quaternization and amide-methylation. Quaternization reaction with CH3I-KHCO3 in methanol was applied to the deprotected N-terminal group of the pentapeptide derivatives affording trimethylammonium group-containing analogs. [Me3+Tyr1,D-Ala2,Leu5]enkephalin and its amide were found to show opioid activity on guinea pig ileium assay only slightly lower than the parent unmethylated peptides. Application of amide-methylation reaction using CH3I-Ag2O in DMF to the protected pentapeptide yielded a pentamethyl derivative in which all of the five N atoms were methylated. Deprotection of the derivative gave pentamethyl analogs of [D-Ala2,Leu5]enkephalin, which showed no significant activity on the guinea pig ileum assay and opiate-receptor binding assay. 相似文献
998.
Shuji Nakamura Shuji Ikegami Kazuo Dairiki Megumi Kochiya Takao Fujimori Norikazu Tamaoki Nobuhiko Tada 《Immunogenetics》1988,28(5):314-319
Mouse lymphocyte alloantigens Ly-19 and Ly-32 are controlled by the genes tightly linked to the Lyb-2 locus on chromosome 4. Despite the similarity in mouse strain distribution patterns, Ly-19 and Ly-32 antigens which have been detected on both B- and T-cell lineages are distinct from Lyb-2 antigen whose expression is restricted to the B cells. In this report, the close linkage of these three loci was confirmed by the typings of three sets of recombinant inbred mice including BXD, CXS, and OXA. Furthermore, the biochemical characterization of these Lyb-2-linked proteins, i. e., Ly-19, Ly-32, and Lyb-2, demonstrated their similarities on a molecular level. Two polypeptides of 45 000 and 95 000 were the components of these three alloantigens. Furthermore, sequential immunoprecipitation experiments indicated that the three alloantigenic determinants were located on the same molecular components. These findings may provide insight into the complexities and functional roles of Lyb-2 gene-cluster products. 相似文献
999.
The activities of serine protease in muscles from normal persons and from patients with progressive muscular and neuromuscular diseases have been determined. A significant increase in the level of serine protease was found in muscle of patients with Duchenne-type muscular dystrophy and with Becker-type muscular dystrophy, but the activity was not increased in muscle of a patient with amyotrophic lateral sclerosis. 相似文献
1000.