全文获取类型
收费全文 | 2118篇 |
免费 | 110篇 |
国内免费 | 2篇 |
专业分类
2230篇 |
出版年
2023年 | 25篇 |
2022年 | 55篇 |
2021年 | 80篇 |
2020年 | 52篇 |
2019年 | 48篇 |
2018年 | 63篇 |
2017年 | 64篇 |
2016年 | 76篇 |
2015年 | 100篇 |
2014年 | 129篇 |
2013年 | 143篇 |
2012年 | 176篇 |
2011年 | 156篇 |
2010年 | 98篇 |
2009年 | 75篇 |
2008年 | 99篇 |
2007年 | 103篇 |
2006年 | 88篇 |
2005年 | 68篇 |
2004年 | 77篇 |
2003年 | 57篇 |
2002年 | 43篇 |
2001年 | 26篇 |
2000年 | 39篇 |
1999年 | 29篇 |
1998年 | 20篇 |
1997年 | 13篇 |
1996年 | 14篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 7篇 |
1992年 | 25篇 |
1991年 | 18篇 |
1990年 | 17篇 |
1989年 | 16篇 |
1988年 | 17篇 |
1987年 | 6篇 |
1986年 | 19篇 |
1985年 | 12篇 |
1984年 | 8篇 |
1983年 | 7篇 |
1982年 | 5篇 |
1981年 | 3篇 |
1980年 | 6篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1972年 | 4篇 |
1970年 | 3篇 |
1969年 | 3篇 |
排序方式: 共有2230条查询结果,搜索用时 15 毫秒
81.
Response surface methodology (RSM) and artificial neural network-real encoded genetic algorithm (ANN-REGA) were employed to develop a process for fermentative swainsonine production from Metarhizium anisopliae (ARSEF 1724). The effect of finally screened process variables viz. inoculum size, oatmeal extract, glucose, and CaCl2 were investigated through central composite design and were further utilized for training sets in ANN with training and test R values of 0.99 and 0.94, respectively. ANN-REGA was finally employed to simulate the predictive swainsonine production with best evolved media composition. ANN-REGA predicted a more precise fermentation model with 103 % (shake flask) increase in alkaloid production compared to 75.62 % (shake flask) obtained with RSM model upon validation. 相似文献
82.
Jatinder Kaur Atul Bhardwaj Sai Kiran Sharma Frank Wuest 《Bioorganic & medicinal chemistry》2013,21(14):4288-4295
A novel group of 1,4-diaryl-substituted triazoles was designed and synthesized by introducing the cyclooxygenase-2 (COX-2) pharmacophore SO2NH2 attached to one aryl ring and various substituents (H, F, Cl, CH3 or OCH3) attached to the other aryl ring. The effects of size and flexibility of the compounds upon COX-1/COX-2 inhibitory potency and selectivity was studied by increasing the size of an alkyl linker chain [(–CH2)n, where n = 0, 1, 2]. In vitro COX-1/COX-2 inhibition studies showed that all compounds (14–18, 21–25 and 28–32) are more potent inhibitors of COX-2 isozyme (IC50 = 0.17–28.0 μM range) compared to COX-1 isozyme (IC50 = 21.0 to >100 μM range). Within the group of 1,4 diaryl-substituted triazoles, 4-{2-[4-(4-chloro-phenyl)-[1,2,3]triazol-1-yl]-ethyl}-benzenesulfonamide (compound 30) displayed highest COX-2 inhibitory potency and selectivity (COX-1: IC50 = >100 μM, COX-2: IC50 = 0.17 μM, SI >588). Molecular docking studies using the catalytic site of COX-1 and COX-2, respectively, provided complementary theoretical support for the obtained experimental biological structure–activity relationship data. Results of molecular docking studies revealed that COX-2 pharmacophore SO2NH2 in compound 30 is positioned in the secondary pocket of COX-2 active site; with the nitrogen atom of the SO2NH2 group being hydrogen bonded to Q192 (N?OC = 2.85 Å), and one of the oxygen atoms of SO2NH2 group forming a hydrogen bond to H90 (SO?N = 2.38 Å). 相似文献
83.
Renu Deswal Ravi Gupta Vivek Dogra Raksha Singh Jasmeet Kaur Abat Abhijit Sarkar Yogesh Mishra Vandana Rai Yelam Sreenivasulu Ramesh Sundar Amalraj Manish Raorane Ram Prasad Chaudhary Ajay Kohli Ashok Prabhakar Giri Niranjan Chakraborty Sajad Majeed Zargar Vishwanath Prasad Agrawal Ganesh Kumar Agrawal Dominique Job Jenny Renaut Randeep Rakwal 《Physiology and Molecular Biology of Plants》2013,19(4):461-477
Plant proteomics has made tremendous contributions in understanding the complex processes of plant biology. Here, its current status in India and Nepal is discussed. Gel-based proteomics is predominantly utilized on crops and non-crops to analyze majorly abiotic (49 %) and biotic (18 %) stress, development (11 %) and post-translational modifications (7 %). Rice is the most explored system (36 %) with major focus on abiotic mainly dehydration (36 %) stress. In spite of expensive proteomics setup and scarcity of trained workforce, output in form of publications is encouraging. To boost plant proteomics in India and Nepal, researchers have discussed ground level issues among themselves and with the International Plant Proteomics Organization (INPPO) to act in priority on concerns like food security. Active collaboration may help in translating this knowledge to fruitful applications. 相似文献
84.
INTRODUCTION:
The relationship between chromosomal non-disjunction leading to aneuploidy and folate metabolism has drawn attention in the recent years. In this study, we examined the polymorphism in the gene encoding the folate metabolizing enzyme methylenetetrahydrofolate reductase (MTHFR), namely, 677 C-T in women having Down syndrome (DS) children.MATERIALS AND METHODS:
The prevalence of these variant genotypes (MTHFR 677 C-T polymorphism) in women having DS children (case mothers) (n = 110) was compared with controls (n = 111) from Punjab. Genotyping was done using the polymerase chain reaction method followed by restriction fragment length polymorphism.RESULTS:
In the present study, 1.8% of case mothers had TT genotype while none of the control mothers showed this genotype. T allele frequency among cases was 0.13 and 0.11 in controls. The Chi-square value showed a non-significant difference between cases and controls.CONCLUSION:
No association has been observed between 677 C-T polymorphism and risk of non-disjunction in case mothers. Detection of polymorphisms in more genes of folate pathway is required to find out the exact cause of non-disjunction. 相似文献85.
Navdeep Kaur Dhami M. Sudhakara Reddy Abhijit Mukherjee 《World journal of microbiology & biotechnology》2013,29(12):2397-2406
Microbially induced calcium carbonate precipitation is a biomineralization process that has various applications in remediation and restoration of range of building materials. In the present study, calcifying bacteria, Bacillus megaterium SS3 isolated from calcareous soil was applied as biosealant to enhance the durability of low energy, green building materials (soil–cement blocks). This bacterial isolate produced high amounts of urease, carbonic anhydrase, extra polymeric substances and biofilm. The calcium carbonate polymorphs produced by B. megaterium SS3 were analyzed by scanning electron microscopy, confocal laser scanning microscopy, X-ray diffraction and Fourier transmission infra red spectroscopy. These results suggested that calcite is the most predominant carbonate formed by this bacteria followed by vaterite. Application of B. megaterium SS3 as biogenic surface treatment led to 40 % decrease in water absorption, 31 % decrease in porosity and 18 % increase in compressive strength of low energy building materials. From the present investigation, it is clear that surface treatment of building materials by B. megaterium SS3 is very effective and eco friendly way of biodeposition of coherent carbonates that enhances the durability of building materials. 相似文献
86.
Ming-Hsu Chen Prabhjot Kaur Bruce Dien Frederick Below Michael L. Vincent Vijay Singh 《World journal of microbiology & biotechnology》2013,29(8):1509-1515
Tropical maize is an alternative energy crop being considered as a feedstock for bioethanol production in the North Central and Midwest United States. Tropical maize is advantageous because it produces large amounts of soluble sugars in its stalks, creates a large amount of biomass, and requires lower inputs (e.g. nitrogen) than grain corn. Soluble sugars, including sucrose, glucose and fructose were extracted by pressing the stalks at dough stage (R4). The initial extracted syrup fermented faster than the control culture grown on a yeast extract/phosphate/sucrose medium. The syrup was subsequently concentrated 1.25–2.25 times, supplemented with urea, and fermented using Saccharomyces cerevisiae for up to 96 h. The final ethanol concentrations obtained were 8.1 % (v/v) to 15.6 % (v/v), equivalent to 90.3–92.2 % of the theoretical yields. However, fermentation productivity decreased with sugar concentration, suggesting that the yeast might be osmotically stressed at the increased sugar concentrations. These results provide in-depth information for utilizing tropical maize syrup for bioethanol production that will help in tropical maize breeding and development for use as another feedstock for the biofuel industry. 相似文献
87.
Amanda J. Martinot Mareike Meythaler Lu-Ann Pozzi Karen Dalecki Boisvert Heather Knight Dennis Walsh Susan Westmoreland Daniel C. Anderson Amitinder Kaur Shawn P. O’Neil 《PloS one》2013,8(3)
Lymphoid tissue immunopathology is a characteristic feature of chronic HIV/SIV infection in AIDS-susceptible species, but is absent in SIV-infected natural hosts. To investigate factors contributing to this difference, we compared germinal center development and SIV RNA distribution in peripheral lymph nodes during primary SIV infection of the natural host sooty mangabey and the non-natural host pig-tailed macaque. Although SIV-infected cells were detected in the lymph node of both species at two weeks post infection, they were confined to the lymph node paracortex in immune-competent mangabeys but were seen in both the paracortex and the germinal center of SIV-infected macaques. By six weeks post infection, SIV-infected cells were no longer detected in the lymph node of sooty mangabeys. The difference in localization and rate of disappearance of SIV-infected cells between the two species was associated with trapping of cell-free virus on follicular dendritic cells and higher numbers of germinal center CD4+ T lymphocytes in macaques post SIV infection. Our data suggests that fundamental differences in the germinal center microenvironment prevent productive SIV infection within the lymph node germinal centers of natural hosts contributing to sustained immune competency. 相似文献
88.
D. P. Singh J. I. S. Khattar Mandeep Kaur Gurdeep Kaur Meenu Gupta Yadvinder Singh 《PloS one》2013,8(1)
This study deals with anilofos tolerance and its mineralization by the common rice field cyanobacterium Synechocystis sp. strain PUPCCC 64. The organism tolerated anilofos up to 25 mg L−1. The herbicide caused inhibitory effects on photosynthetic pigments of the test organism in a dose-dependent manner. The organism exhibited 60, 89, 96, 85 and 79% decrease in chlorophyll a, carotenoids, phycocyanin, allophycocyanin and phycoerythrin, respectively, in 20 mg L−1 anilofos on day six. Activities of superoxide dismutase, catalase and peroxidase increased by 1.04 to 1.80 times over control cultures in presence of 20 mg L−1 anilofos. Glutathione content decreased by 26% while proline content was unaffected by 20 mg L−1 anilofos. The test organism showed intracellular uptake and metabolized the herbicide. Uptake of herbicide by test organism was fast during initial six hours followed by slow uptake until 120 hours. The organism exhibited maximum anilofos removal at 100 mg protein L−1, pH 8.0 and 30°C. Its growth in phosphate deficient basal medium in the presence of anilofos (2.5 mg L−1) indicated that herbicide was used by the strain PUPCCC 64 as a source of phosphate. 相似文献
89.
90.
Sumon Kumar Das Mohammod Jobayer Chisti Sayeeda Huq Mohammad Abdul Malek Lana Vanderlee Guddu Kaur Mohammed Abdus Salam Tahmeed Ahmed Abu Syed Golam Faruque Abdullah Al Mamun 《PloS one》2013,8(8)