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991.
Okazaki pieces grow opposite to the replication fork direction during simian virus 40 DNA replication. 总被引:15,自引:4,他引:11 下载免费PDF全文
Simian virus 40 replicating DNA was pulse labeled with alpha-32P-dATP using an acellular DNA replication system. Nascent DNA chains of less than 200 nucleotides (Okazaki pieces) were then isolated from the denatured replicating DNA by electrosieving through a polyacrylamide gel column. The purified Okazaki pieces were hybridized to separated strands of Bg1(1)+Hpa1 simian virus 40 DNA restriction fragments immobilized on nitrocellulose filters. Only strands with polarity of the DNA replication fork direction hybridized with Okazaki pieces. Hence, Okazaki pieces in simian virus 40 are synthesized against the DNA replication fork direction. 相似文献
992.
K J Kaufmann K M Petty P L Dutton P M Rentzepis 《Biochemical and biophysical research communications》1976,70(3):839-845
Following picosecond light activation, the bacteriochlorophyll and bacteriopheophytin complement of reaction centers depleted of ubiquinone behaves as though it has no primary electron acceptor; the excited intermediary state formed in <10 ps lasts >1 ns. Addition of ubiquinone-10 reconstitutes the very rapid electron transfer rates from the excited intermediary state to ubiquinone; the kinetics and rate are similar to that encountered in the untreated reaction centers. Interpretation of the data presented suggests that ubiquinone is the immediate electron acceptor from BPh?. This is consistent with the model for the primary reactions leading to [(BChl)2?BPh]Q?. 相似文献
993.
The technique of laser Doppler electrophoresis was applied for the study of the surface charge properties of (Na+,+)-ATPase containing microsomal vesicles derived from guinea-pig kidney. The influence of pH, the screening and binding of uni- and divalent cations and the binding of ATP show: (1) one net negative charge per protein unit with a ; (2) deviation from the Debye relation between surface potential and ionic strength for univalent cations, with no difference in the effect of Na+ and K+; (3) Mg2+ binds with an association constant of while ATP binds with an apparent for 1 mM Nacl, 0.2 mM KCI, 0.1 mM MgCl2, 0.1 mM Tris-HCI (pH 7.3). The binding is weaker at higher Mg2+ concentrations. There is no ATP binding in the absence of Mg2+. In addition, the average vesicle size derived from the linewidth of the quasi-elastic light scattering spectrum is . In the presence of ATP a reduction in size is observed. 相似文献
994.
Norbert Kaufmann Horst-Helwig Reidl Jochen R. Golecki Augusto F. Garcia Gerhart Drews 《Archives of microbiology》1982,131(4):313-322
Aerobically in the dark grown cultures of Rhodopseudomonas capsulata were shifted to low oxygen partial pressure for 30 min and afterwards to phototrophic conditions (anaerobic, light). During 210 min of adaptation to a phototrophic mode of life the bacteriochlorophyll (BChl) concentration increased 53-fold (doubling time 40 min) and the carotenoid content six fold. Growth was delayed. The light membrane fraction from chemotrophic and induced phototrophic cells contained low concentrations of small photosynthetic units (reaction center+light harvesting BChl B870), and low respiratory activities, especially of succinatecytochrome c oxidase. The heavy membrane fraction, i.e. the intracytoplasmic chromatophore fraction, increased during adaptation approximately 9-fold in surface area per cell, 42-fold in BChl content, 7-fold in reaction center content and 6-fold in the size of the photosynthetic unit.Phospholipid and fatty acid content and patterns changed slightly during adaptation.Non-standard Abbreviations BChl
bacteriochlorphyll
- R.
Rhodopseudomonas 相似文献
995.
Prof. Dr. Gerd Flajs Dipl.-Geol. Manfred Vigener Prof. Dr. Helmut Keupp Prof. Dr. Dieter Meischner Dipl.-Geol. Fritz Neuweiler PD Dr. Josef Paul Prof. Dr. Joachim Reitner Dr. Klaus Warnke PD Dr. Helmut Weller Dipl.-Geol. Patrick Dingle Dipl.-Geol. Christian Hensen Prof. Dr. Priska Schäfer Dr. Pascale Gautret Prof. Dr. Reinhold R. Leinfelder PD Dr. Hansmartin Hüssner Dipl.-Geol. Bernd Kaufmann 《Facies》1995,32(1):1-69
Summary This research report contains nine case studies (part II to X) dealing with Palaeozoic and Mesozoic mud mounds, microbial
reefs, and modern zones of active micrite production, and two parts (I and XI) summarizing the major questions and results.
The formation of different types ofin situ formed micrites (automicrites) in close association with siliceous sponges is documented in Devonian, Carboniferous, Triassic,
Jurassic and Cretaceous mounds and suggests a common origin with a modern facies found within reef caves. Processes involved
in the formation of autochthonous micrites comprise: (i) calcifying mucus enriched in Asp and Glu, this type presumably is
linked to the formation of stromatolites, thrombolites and massive fabrics; (ii) protein-rich substances within confined spaces
(e.g. microcavities) result in peloidal pockets, peloidal coatings and peloidal stromatolites, and (iii) decay of sponge soft
tissues, presumably enriched with symbiotic bacteria, lead to the micropeloidal preservation of parts of former sponge bodies.
As a consequence, there is strong evidence that the primary production of micrite in place represents the initial cause for
buildup development. The mode of precipitation corresponds to biologically-induced, matrix-mediated mineralization which results
in high-Mg-calcites, isotopically balanced with inorganic cements or equilibrium skeletal carbonates, respectively. If distinct
automicritic fabrics are absent, the source or origin of micrite remains questionable. However, the co-occurring identifiable
components are inadequate, by quantity and physiology, to explain the enhanced accumulation of fine-grained calcium carbonate.
The stromatolite reefs from the Permian Zechstein Basin are regarded as reminiscent of ancestral (Precambrian) reef facies,
considered the precursor of automicrite/sponge buildups. Automicrite/sponge buildups represent the basic Phanerozoic reef
type. Analogous facies are still present within modern cryptic reef habitats, where the biocalcifying carbonate factory is
restricted in space. 相似文献
996.
Identification and quantification of Bifidobacterium species isolated from food with genus-specific 16S rRNA-targeted probes by colony hybridization and PCR. 总被引:12,自引:0,他引:12 下载免费PDF全文
A Bifidobacterium genus-specific target sequence in the V9 variable region of the 16S rRNA has been elaborated and was used to develop a hybridization probe. The specificity of this probe, named lm3 (5'-CGGGTGCTI*CCCACTTTCATG-3'), was used to identify all known type strains and distinguish them from other bacteria. All of the 30 type strains of Bifidobacterium which are available at the German culture collection Deutsche Sammlung von Mikroorganismen und Zellkulturen, 6 commercially available production strains, and 34 closely related relevant strains (as negative controls) were tested. All tested bifidobacteria showed distinct positive signals by colony hybridization, whereas all negative controls showed no distinct dots except Gardnerella vaginalis DSM4944 and Propionibacterium freudenreichii subsp. shermanii DSM4902, which gave slight signals. Furthermore, we established a method for isolation and identification of bifidobacteria from food by using a PCR assay without prior isolation of DNA but breaking the cells with proteinase K. By this method, all Bifidobacterium strains lead to a DNA product of the expected size. We also established a quick assay to quantitatively measure Bifidobacterium counts in food and feces by dilution plating and colony hybridization. We were able to demonstrate that 2.1 x 10(6) to 2.3 x 10(7) colonies/g of sour milk containing bifidobacteria hybridized with the specific nucleotide probe. With these two methods, genus-specific colony hybridization and genus-specific PCR, it is now possible to readily and accurately detect any bifidobacteria in food and fecal samples and to discriminate between them and members of other genera. 相似文献
997.
Introduction of intersubunit disulfide bonds in the membrane-distal region of the influenza hemagglutinin abolishes membrane fusion activity. 总被引:12,自引:0,他引:12
L Godley J Pfeifer D Steinhauer B Ely G Shaw R Kaufmann E Suchanek C Pabo J J Skehel D C Wiley 《Cell》1992,68(4):635-645
Influenza virus hemagglutinin (HA) mediates viral entry into cells by a low pH-induced membrane fusion event in endosomes. A number of structural changes occur throughout the length of HA at the pH of fusion. To probe their significance and their necessity for fusion activity, we have prepared a site-directed mutant HA containing novel intersubunit disulfide bonds designed to cross-link covalently the membrane-distal domains of the trimer. These mutations inhibited the low pH-induced conformational changes and prevented HA-mediated membrane fusion; conditions that reduced the novel disulfide bonds restored membrane fusion activity. We conclude that structural rearrangements in the membrane distal region of the HA are required for membrane fusion activity. 相似文献
998.
Chronic low-frequency stimulation of rat fast-twitch muscle induces 3.7-fold elevations in cytochrome c oxidase activity, but remains without effect on carbonic anhydrase III (CAIII) mRNA and protein. This is in contrast with the situation in the rabbit where chronic stimulation elicits more than 10-fold elevations in CAIII activity and mRNA content which coincide with an enhanced expression of the slow myosin heavy chain (HCI). Since chronic stimulation of rat muscle does not enhance the expression of HCI, we conclude that CAIII is expressed in parallel with HCI and, therefore, is present only in type I and C fibers. 相似文献
999.
I Harbitz B Chowdhary P D Thomsen W Davies U Kaufmann S Kran I Gustavsson K Christensen J G Hauge 《Genomics》1990,8(2):243-248
Several studies point to the possibility that malignant hyperthermia (MH) in pigs is caused by a defect in the calcium release channel (CRC) of skeletal muscle sarcoplasmic reticulum. The locus for MH is closely linked to the glucosephosphate isomerase (GPI) locus, near the centromere of chromosome 6. We demonstrate synteny of the genes for CRC and GPI using somatic cell hybrid lines, and assign the CRC gene to chromosome 6p11----q21 by in situ hybridization. 相似文献
1000.
Synopsis The relationship between respiration and swimming speed of larvae and juveniles (2–100 mg fresh mass) of Danube bleak, Chalcalburnus chalcoides (Cyprinidae), was measured at 15° and 20° C under hypoxic (50% air saturation), normoxic, and hyperoxic (140% air saturation) conditions. In a flow-tunnel equipped with a flow-through respirometer the animals swam at speeds of up to 8 lengths · s-1; speeds were sustained for at least two minutes. The mass specific standard, routine, and active respiration rates declined with increasing body mass at both temperatures. Metabolic intensity increased with temperature, but also the critical swimming speed (at which oxygen uptake reached its maximum) was higher at 20° than at 15° C by about 30%. Nevertheless, the oxygen debt incurred by the fish at the highest speeds was about 40%, and the net cost of swimming about 32%, lower at 20° than at 15°C. The standard metabolic rate was more strongly dependent on temperature (Q10 around 2.5) than the maximum active rate (Q10 below 2). Whereas standard and routine respiration rates were well regulated over the pO2-range investigated (8.5–25.8 kPa), the active rates showed a conformer-like pattern, resulting in factorial scopes for activity between 2 and 4. Under hypoxia, the critical swimming speed was lower than under normoxia by about 1.51 · s-1, but the net cost of swimming was also lower by about 30%. On the other hand, hyperoxia neither increased the swimming performance nor did it lead to a further increase of the metabolic cost of swimming. The hypoxia experiments suggest that in response to lowered tensions of ambient oxygen maintenance functions of metabolism not directly related to swimming may be temporarily reduced, leading to increased apparent swimming efficiency under these conditions. The responses of the larvae of Danube bleak to low temperature and low ambient oxygen are discussed in terms of the metabolic strategies by which energy-limited animals meet the challenge of environmental deterioration. 相似文献